Detection and molecular characterization of cactus witches'-broom disease associated with a group 16SrII phytoplasma in northern areas of China

Li, Zhengnan; Zhang, Lei; Liu, Ping; Bai, Yao-Bo; Yang, Xiaofei; Wu, Yunfeng

doi:10.1590/s1982-56762012000300008

Cited by 9 publications

(4 citation statements)

References 10 publications

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“…The reason of the unspecific digestion was kept unknown. It is known that phytoplasmas of 16SrI are the main group occurred in China (Li, Zhang, Liu, et al, 2012), and various subgroups and novel subgroups were discovered (Li, Zhang, Liu, et al, 2012; Li et al., 2014; Luan et al., 2018; Tseng et al., 2016; Zhang et al., 2019). In this work, we extended the queue of 16SrI subgroups with another two novel subgroups of 16SrI‐AK and ‐AL.…”

Section: Discussionmentioning

confidence: 99%

Campsisgrandiflora as a new host species harbouring two novel 16SrI subgroups of phytoplasmas

Sun

Zhang

2020

Forest Pathology

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In September 2019, two diseased plants of Campsis grandiflora showing the main symptom of witches' ‐broom (CgWB) were found in a nursery garden in Yangling, Shaanxi province, China. Partial 16S ribosomal RNA (F2nR2 region) and ribosomal protein (rp) genes of phytoplasmas were generated from the symptomatic plants by PCR amplification, and phytoplasma bodies were observed in the sieve tube elements of the CgWB samples under a transmission electron microscope, indicating phytoplasma infection in the two CgWB plants. Restriction fragment length polymorphism analysis of the F2nR2 region and similarity coefficient results suggested that the two associated phytoplasmas belong to two novel subgroups of 16SrI (aster yellows) group, designated as AK and AL. On the reconstructed phylogenetic trees based on F2nR2 regions and rp genes of phytoplasmas, respectively, the CgWB‐associated phytoplasmas clustered together with members of 16SrI subgroups. This was the first record of phytoplasmas infecting C. grandiflora worldwide.

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Section: Discussionmentioning

confidence: 99%

Campsisgrandiflora as a new host species harbouring two novel 16SrI subgroups of phytoplasmas

Sun

Zhang

2020

Forest Pathology

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“…Groups 16SrI and ‐V are important in northern areas of China, and group 16SrII is important in southern areas of China (Li et al. ). This, however, is the first record of group 16SrVI phytoplasma in China, which, to our knowledge, has great significance for plant pathology research in China.…”

Section: Resultsmentioning

confidence: 99%

Detection and Identification of Group 16SrVI Phytoplasma in Willows in China

Zhang

Du³

et al. 2012

Journal of Phytopathology

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In 2010 and 2011, willow proliferation disease was observed in Erdos, Inner Mongolia, China. The phytoplasma-specific 16S rRNA gene fragment of 1.2 kb was amplified by a nested PCR with universal primer pair P1/P7 followed by R16F2n/R2. Phylogenetic and virtual RFLP analyses revealed that the phytoplasma associated with willow proliferation was a member of subgroup 16SrVI-A. The field survey indicated that the incidence of willow proliferation in Erdos was approximately 36.84%. To our knowledge, this is the first record of group 16SrVI phytoplasma infecting willow in China. *The first two authors contributed equally to this research.

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“…In 2009, locust trees witches' -broom disease was found in Taian, Shandong (Yu et al, 2012). In July 2010, the cactus witches' -broom disease caused by the 16SrII-C subgroup was first reported in Yangling, Shanxi (Li et al, 2012). Tomato yellowing disease caused by a 16SrII-A phytoplasma was first reported in Yunnan Province in 2011 (Dong et al, 2013).…”

Section: Historical Backgroundmentioning

confidence: 99%

The Diversity, Distribution and Status of Phytoplasma Diseases in China

Wang¹,

Zhang²,

Li³

et al. 2022

Front. Sustain. Food Syst.

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Phytoplasmas are important prokaryotic pathogenic bacteria without cell walls, which were formerly known as mycoplasma-like organisms, and belong to the Mollicutes class, Candidatus Phytoplasma genus. They are widely distributed in plants and insects, and can cause serious diseases in important food crops, vegetables, fruit trees, ornamental plants and trees, resulting in huge economic losses. To date, more than 100 phytoplasma diseases have been reported in China, which are distributed throughout the country. Jujube witches'-broom, paulownia witches'-broom, wheat blue dwarf, banana bunchy top, sugarcane white leaf, rice orange leaf and mulberry dwarf represent the phytoplasma diseases causing the most serious damage in China. New phytoplasma diseases and their strains are being reported continuously, indicating that phytoplasmas are more diverse than previously thought. Phytoplasmas are mainly transmitted by insect vectors, such as leafhopper and planthopper, and can also be spread by grafting or Cuscuta australis (known as dodder). Mixed infections of phytoplasmas and viruses, bacteria, and spiroplasmas have also become a serious problem in several crops and are responsible for more synergistic losses. With the continuous development and improvement of technology, molecular biological detection has become the main technique for phytoplasma detection and identification. Currently, research on phytoplasma diseases in China mainly focuses on pathogen identification and classification, and insect vector and host diversity; however, there is less focus on pathogenicity, comparative genomics, and effect factors. More research attention has been paid to wheat blue dwarf phytoplasma, paulownia witches'-broom phytoplasma, jujube witches'-broom phytoplasma, and sugarcane white leaf phytoplasma. Other phytoplasma diseases have been reported; however, there have been no in-depth studies. In this paper, the history and present situation of phytoplasma research, and the status, distribution, and diversity of phytoplasma diseases are summarized, and some possible research directions of phytoplasma in the future in China are proposed.

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Detection and molecular characterization of cactus witches'-broom disease associated with a group 16SrII phytoplasma in northern areas of China

Cited by 9 publications

References 10 publications

Campsisgrandiflora as a new host species harbouring two novel 16SrI subgroups of phytoplasmas

Campsisgrandiflora as a new host species harbouring two novel 16SrI subgroups of phytoplasmas

Detection and Identification of Group 16SrVI Phytoplasma in Willows in China

The Diversity, Distribution and Status of Phytoplasma Diseases in China

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