<i>Campsis</i><i>grandiflora</i> as a new host species harbouring two novel 16SrI subgroups of phytoplasmas

Li, Zheng‐Nan; Sun, Pu; Zhang, Lei

doi:10.1111/efp.12619

Cited by 5 publications

(3 citation statements)

References 34 publications

(38 reference statements)

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“…No virus-like particles were detected. However, similar to phytoplasmas previously reported in some studies (Zhang et al 2013;Rao 2018;Li et al 2020), some thalli,were observed in phloem sieve cells (Figure 4). All thalli in the sieve tube cells of the leaf were subglobose, and ranged from 200 to 800 nm in diameter (mean 350 nm).…”

Section: Symptoms Of Spld In Chinasupporting

confidence: 88%

Detection and molecular identification of a 16SrI group phytoplasma associated with sisal purple leafroll disease

Wang¹,

Wu²,

Xi³

et al. 2023

Plant Prot. Sci.

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Section: Symptoms Of Spld In Chinasupporting

confidence: 88%

Detection and molecular identification of a 16SrI group phytoplasma associated with sisal purple leafroll disease

Wang¹,

Wu²,

Xi³

et al. 2023

Plant Prot. Sci.

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“…Similarity coefficients derived from virtual RFLP analysis of 16S rRNA genes of strain PLH102-1 and other 16SrI subgroups were less than or equal to 0.97, the threshold for a new subgroup delineation [ 26 ]. Therefore, strain PLH102-1 was designated as a new subgroup 16SrI-AO ( Table 2 [ 10 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 ], some of previously reported 16SrI subgroups were reassigned due to duplication).…”

Section: Resultsmentioning

confidence: 99%

Identification of Phytoplasmas Representing Multiple New Genetic Lineages from Phloem-Feeding Leafhoppers Highlights the Diversity of Phytoplasmas and Their Potential Vectors

et al. 2021

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Phytoplasmas are obligate transkingdom bacterial parasites that infect a variety of plant species and replicate in phloem-feeding insects in the order Hemiptera, mainly leafhoppers (Cicadellidae). The insect capacity in acquisition, transmission, survival, and host range directly determines the epidemiology of phytoplasmas. However, due to the difficulty of insect sampling and the lack of follow-up transmission trials, the confirmed phytoplasma insect hosts are still limited compared with the identified plant hosts. Recently, quantitative polymerase chain reaction (qPCR)-based quick screening of 227 leafhoppers collected in natural habitats unveiled the presence of previously unknown phytoplasmas in six samples. In the present study, 76 leafhoppers, including the six prescreened positive samples, were further examined to identify and characterize the phytoplasma strains by semi-nested PCR. A total of ten phytoplasma strains were identified in leafhoppers from four countries including South Africa, Kyrgyzstan, Australia, and China. Based on virtual restriction fragment length polymorphism (RFLP) analysis, these ten phytoplasma strains were classified into four distinct ribosomal (16Sr) groups (16SrI, 16SrIII, 16SrXIV, and 16SrXV), representing five new subgroups (16SrI-AO, 16SrXIV-D, 16SrXIV-E, 16SrXIV-F, and 16SrXV-C). The results strongly suggest that the newly identified phytoplasma strains not only represent new genetic subgroup lineages, but also extend previously undiscovered geographical distributions. In addition, ten phytoplasma-harboring leafhoppers belonged to seven known leafhopper species, none of which were previously reported insect vectors of phytoplasmas. The findings from this study provide fresh insight into genetic diversity, geographical distribution, and insect host range of phytoplasmas. Further transmission trials and screening of new potential host plants and weed reservoirs in areas adjacent to collection sites of phytoplasma harboring leafhoppers will contribute to a better understanding of phytoplasma transmission and epidemiology.

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“…Approximately 36 subgroups were described for ribosomal group I (Li, Sun, & Zhang, 2020; Wei et al, 2021), and 16SrI‐B was the most prevalent, occurring in America, Europe and Asia (Marcone et al, 2000; Pérez‐López et al, 2016). The phytoplasmas identified in the present study were closely related with the 16SrI‐B ribosomal subgroup and were found to be associated with a diverse range of crops exhibiting various symptoms: leaf discoloration in field pea and lupine; deformed flowers in rape and cauliflower; fasciation in sugar beet and mustard; bushy and stunted growth in maize, marigold and juniper.…”

Section: Discussionmentioning

confidence: 99%

Intra and extragenomic variation between 16S rRNA genes found in 16SrI‐B‐related phytopathogenic phytoplasma strains

Zwolińska

Borodynko-Filas

2021

Annals of Applied Biology

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Phytoplasmas are bacterial pathogens that pose a threat to cereal and vegetable production worldwide. However, there is limited information regarding the occurrences of phytoplasmas in Central Europe. In this study we examined samples taken from crop and ornamental plants showing symptoms indicative of phytoplasma infection. Phytoplasmas related to 'Candidatus Phytoplasma asteris' were detected in eight plant species (carrot, lupine, pea, cauliflower, mustard, maize, juniper and marigold). Based on the restriction fragment length polymorphism analysis of the 16S rDNA, the phytoplasmas were classified as subgroup 16SrI-B, 16SrI-L or a complex strain, which harboured the I-B and I-L-type 16S rRNA genes. Individual 16S rDNA sequences were analysed from those heterogeneous strains and as many as five genetic variants were distinguished. By operon-specific amplification of the 16S rRNA genes with primers complementary to RP166 phytoplasma genome, we detected 16SrI-L/B phytoplasma strains in the multivariant samples. The I-L-type sequences were amplified from rrnA and the I-B-type sequences were amplified from rrnB.Molecular phylogeny based on the 16S rRNA showed that all the detected phytoplasma variants were clustered with a 'Ca. Phytoplasma asteris' subclade and were closely related to the 16SrI-B subgroup. Despite the variability of 16S rDNA, the strains from all the eight crops shared identical tuf gene sequences. We report, for the first time, 16SrI-B phytoplasma infecting lupine, field pea, cauliflower and mustard, 16SrI-L phytoplasma infecting maize and field pea and 16SrI-L/B phytoplasma infecting carrot, lupine and marigold. We further demonstrate variability in the 16S rRNA genes found in the 16SrI-B-related phytoplasma genomes worldwide. For clearer and more unambiguous nomenclature, we propose that in the future, the more intuitive subgroup 16SrI-L/B be used for heterogenic phytoplasma strains instead of 16SrI-(B/L)L.

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Campsisgrandiflora as a new host species harbouring two novel 16SrI subgroups of phytoplasmas

Cited by 5 publications

References 34 publications

Detection and molecular identification of a 16SrI group phytoplasma associated with sisal purple leafroll disease

Detection and molecular identification of a 16SrI group phytoplasma associated with sisal purple leafroll disease

Identification of Phytoplasmas Representing Multiple New Genetic Lineages from Phloem-Feeding Leafhoppers Highlights the Diversity of Phytoplasmas and Their Potential Vectors

Intra and extragenomic variation between 16S rRNA genes found in 16SrI‐B‐related phytopathogenic phytoplasma strains

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