Molecular characterization of a phytoplasma of group 16SrIX related to 'Ca. Phytoplasma phoenicium' in periwinkle in Brazil

Barbosa, J. C.; Eckstein, Bárbara; Filho, Armando Bergamin; Bedendo, Ivan Paulo; Kitajima, Elliot Watanabe

doi:10.1590/s1982-56762012000200005

Cited by 10 publications

(7 citation statements)

References 14 publications

(15 reference statements)

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“…In Puerto Rico, this plant is commonly used in private gardens as an ornamental plant. In tropical areas, periwinkle plants may serve as a natural reservoir for this pathogen, spreading the disease to other plant species of economic importance, such as citrus trees (Barbosa et al 2012), although we did not observe on symptomatic periwinkle plants leafhoppers that could be potential insect vectors. Circumstantial evidence suggests that PPWB phytoplasma in several plant species has emerged as a significant problem in South and Central America (Kenyon et al 1998;Teixeira et al 2008), and this microorganism can be spread to neighbouring countries.…”

Section: In Colombia For Coffee In W H I C H T H E S E S Y M P T O Mcontrasting

confidence: 60%

Detection and molecular characterisation of a group 16SrIX phytoplasma infecting citrus (Citrus sinensis and C. limon), coffee (Coffea arabica), periwinkle (Catharanthus roseus), and tabebuia (Tabebuia heterophylla) in Puerto Rico

Caicedo

Rivera-Vargas

Segarra

et al. 2015

Australasian Plant Dis. Notes

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Detection and molecular characterisation of a group 16SrIX phytoplasma infecting citrus (Citrus sinensis and C. limon), coffee (Coffea arabica), periwinkle (Catharanthus roseus), and tabebuia (Tabebuia heterophylla) in Puerto Rico Abstract Few studies have determined the presence of phytoplasmas in important crops in Puerto Rico. Disease symptoms resembling those caused by phytoplasmas were observed in pigeon pea (Cajanus cajan), periwinkle (Catharanthus roseus), tabebuia (Tabebuia heterophylla), Spanish lime (Melicoccus bijugatus), ixora (Ixora coccinea), mango (Mangifera indica), cactus (Opuntia spp.), citrus trees (Citrus spp.), and coffee (Coffea arabica). Sixty-two samples from these species were tested using conventional PCR to amplify the 16S rRNA and ribosomal protein genes (rpIVrpsC). Fifty-one percent of the tested samples (corresponding to periwinkle, pigeon pea, citrus, coffee and tabebuia) were positive for phytoplasmas, with amplicons of 0.8 (16S rRNA gene) and 1.2 kb (rpIV-rpsC genes), depending upon primers used in PCRs. For both genetic loci, DNA sequences showed 99 % identity with pigeon pea witches' broom phytoplasma (PPWB). Due to the lack of studies of potential insect vectors, common Auchenorrhyncha species were sweep-collected from pigeon pea and citrus and tested for phytoplasma. Of nine insect genera collected, Empoasca kraemeri (Cicadellidae), Melormenis antillarum (Flatidae), and Colpoptera maculifrons (Issidae) were positive for PPWB based on results from conventional PCR and DNA sequence analysis. The findings indicate that these insects fed upon the aforementioned plant species, ingesting contents of phloem, and may act as potential phytoplasma vectors in the field. These are first reports of PPWB phytoplasma infections in citrus species (C. sinensis and C. limon), coffee, periwinkle and tabebuia, and in insects (E. kraemeri, M. antillarum and C. maculifrons) for Puerto Rico.

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Section: In Colombia For Coffee In W H I C H T H E S E S Y M P T O Mcontrasting

confidence: 60%

Detection and molecular characterisation of a group 16SrIX phytoplasma infecting citrus (Citrus sinensis and C. limon), coffee (Coffea arabica), periwinkle (Catharanthus roseus), and tabebuia (Tabebuia heterophylla) in Puerto Rico

Caicedo

Rivera-Vargas

Segarra

et al. 2015

Australasian Plant Dis. Notes

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“…The symptoms in both geographic locations are consistent with those previously reported for the host and subgroup 16SrIX-A, such as virescence and yellowing. Natural infection of this plant species by group 16SrIX-A was reported previously in Colombia (Duduk et al, 2008), Brazil (Barbosa et al, 2012) and Puerto Rico (Caicedo et al, 2015).…”

Section: Discussionsupporting

confidence: 71%

“…Catharanthus roseus has been reported as a natural host for phytoplasmas groups 16SrI, 16SrIII, 16SrIX, 16SrXV, 16SrXIII, in North and South America, and the Caribbean islands, but not from the Central American region (Lee et al, 1998b;Lee, Davis, & Gundersen-Rindall, 2000;Montano, Dally, Davis, Pimentel, & Brioso, 2001b;Torres, Galdeano, Docampo, & Conci, 2004;Duduk, Mejia, Calari, & Bertaccini, 2008;Barbosa, Eckstein, Bergamin Filho, Bedendo, & Kitajima, 2012;Galdeano, Guzmán, Fernández, & Conci, 2013;Dumonceaux, Green, Hammond, Pérez, & Olivier, 2014;Caicedo, Rivera-Vargas, Segarra, & Davis, 2015;Davis, Harrison, Zhao, Wei, & Dally, 2016;Pérez-López, Luna-Rodríguez, Olivier, & Dumonceaux, 2016a). According to Montano et al (2001b), C. roseus is a well-known experimental host for phytoplasmas, however few incidences of natural infections have been reported and its role related to natural dissemination and disease spread is uncertain.…”

Section: Discussionmentioning

confidence: 99%

Catharanthus roseus (Apocynaceae) naturally infected with diverse phytoplasmas in Costa Rica

Villalobos

Bottner-Parker

Lee

et al. 2019

RBT

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Phytoplasmas (class Mollicutes) are causal agents of plant diseases with an economic impact on crops or threatening local biodiversity. A survey was conducted from 2012 to 2016 on infected Catharanthus roseus plants that exhibited symptoms reminiscent of phytoplasma infection throughout Costa Rica. A total of 73 plants were collected exhibiting symptoms such as virescence, phyllody, axillary proliferation, little leaf, leaf malformation, chlorosis, or yellowing. All samples were tested by nested PCR using phytoplasma universal and specific primer pairs. Phytoplasma infection was detected in 52 (71.2 %) of the plants collected. Phytoplasmas of six subgroups belonging to 16Sr groups I, III, IX, XIII and XV were identified based on sequencing and in silico RFLP analyses. ´Candidatus Phytoplasma asteris´ (16SrI) was the predominant group among the positive samples (n = 30) showing variety of symptoms and wide distribution from sea level to ca. 1 400 masl in six of the seven Costa Rican provinces. Group 16SrIII was the second most abundant (14 samples); and the remaining three groups were seldom found in C. roseus (8 samples). Moreover, group 16SrXIII phytoplasma was detected for the first time in the country. To the best of our knowledge, this is the first report of natural infection of C. roseus with phytoplasma subgroups 16SrI-B, 16SrI-P, 16SrIII-F, 16SrIX-F, 16SrXIII-A, and 16SrXV-B in Costa Rica and Central America.

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“…In Mexico, the species B. odorata was reported as a probable alternative host of the 16SrIX phytoplasma, as well as the green manure crop Cajanus cajan (Martínez-Bustamante et al 2018). Catharanthus roseus is also a host for 16SrIX phytoplasma in Brazil (Barbosa et al 2012). The low titer found in the weeds in our case is about 10 times less than reported in sweet oranges and in both cases, much lower than the titer reached in sunn hemp (Wulff et al 2015).…”

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confidence: 43%

Survey of 16SrIX phytoplasmas associated with HLB-symptoms in weeds and leafhoppers at citrus orchards

Lopes-da-Silva

Martins

Wulff

et al. 2020

Trop. plant pathol.

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Citrus plants presenting the typical huanglongbing (HLB) symptoms have been associated to 16SrIX phytoplasma in the States of São Paulo (SP), Minas Gerais (MG), Bahia (BA) and, more recently, Distrito Federal (DF), Brazil. Although in SP and MG states 'Candidatus Liberibacter asiaticus' is more commonly associated with HLB, single infection by phytoplasmas also occurs. To date, only 16SrIX phytoplasma induces HLB symptoms in BA and DF, a region free of 'Ca. Liberibacter spp.'. The objective of this work was to survey for alternative hosts and potential vectors of the 16SrIX phytoplasma in citrus orchards from DF using quantitative real-time PCR (qPCR) detection. Fifty-three specimens encompassing fifteen species of weeds were collected from three Tahiti lime (Citrus latifolia Tanaka) and Ponkan mandarin (Citrus reticulata Blanco) orchards. Only five plants, one Euphorbia sp., one Sida sp., and three Bidens pilosa were positive for the presence of the 16SrIX phytoplasma. Regarding the leafhoppers, 16SrIX phytoplasma was detected in 11/ 38 specimens of Scaphytopius sp. and not detected in 32 specimens of Planicephalus flavicosta. These results suggest that spontaneous vegetation and the leafhoppers Scaphytopius may harbor 16SrIX phytoplasma that can further infect citrus orchards. The capacity of Scaphytopius insects to vector the phytoplasma to citrus plant should be better investigated.

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Molecular characterization of a phytoplasma of group 16SrIX related to 'Ca. Phytoplasma phoenicium' in periwinkle in Brazil

Cited by 10 publications

References 14 publications

Detection and molecular characterisation of a group 16SrIX phytoplasma infecting citrus (Citrus sinensis and C. limon), coffee (Coffea arabica), periwinkle (Catharanthus roseus), and tabebuia (Tabebuia heterophylla) in Puerto Rico

Detection and molecular characterisation of a group 16SrIX phytoplasma infecting citrus (Citrus sinensis and C. limon), coffee (Coffea arabica), periwinkle (Catharanthus roseus), and tabebuia (Tabebuia heterophylla) in Puerto Rico

Catharanthus roseus (Apocynaceae) naturally infected with diverse phytoplasmas in Costa Rica

Survey of 16SrIX phytoplasmas associated with HLB-symptoms in weeds and leafhoppers at citrus orchards

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