Biocontrole de doenças de parte aérea do tomateiro por Nocardioides thermolilacinus

Filho, Renato Carrer; Romeiro, R. S.; Garcia, Flávio Augusto O.

doi:10.1590/s1982-56762008000600010

Cited by 13 publications

(4 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Additionally, a higher number of genera were induced from week 6 onwards. Some of these genera contain species previously reported to be involved in the N-cycle, e.g., Nitratireductor, Devosia, Nitrospira , and Taibaiella (Rivas et al, 2002; Hoque et al, 2011; Penton et al, 2013; Zhang et al, 2013; Kox and Jetten, 2015) and some contain species that can act as biocontrol agent, e.g., Streptomyces and Nocardioides (Carrer et al, 2008; Saharan and Nehra, 2011; Viaene et al, 2016). These so-called plant-growth promoting rhizobacteria (PGPR) have been extensively studied because of their beneficial effects on plant growth and health (Berendsen et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Dynamics in the Strawberry Rhizosphere Microbiome in Response to Biochar and Botrytis cinerea Leaf Infection

Tender

Haegeman²,

Vandecasteele³

et al. 2016

Front. Microbiol.

View full text Add to dashboard Cite

Adding biochar, the solid coproduct of biofuel production, to peat can enhance strawberry growth, and disease resistance against the airborne fungal pathogen Botrytis cinerea. Additionally, biochar can induce shifts in the strawberry rhizosphere microbiome. However, the moment that this biochar-mediated shift occurs in the rhizosphere is not known. Further, the effect of an above-ground infection on the strawberry rhizosphere microbiome is unknown. In the present study we established two experiments in which strawberry transplants (cv. Elsanta) were planted either in peat or in peat amended with 3% biochar. First, we established a time course experiment to measure the effect of biochar on the rhizosphere bacterial and fungal communities over time. In a second experiment, we inoculated the strawberry leaves with B. cinerea, and studied the impact of the infection on the rhizosphere bacterial community. The fungal rhizosphere community was stabilized after 1 week, except for the upcoming Auriculariales, whereas the bacterial community shifted till 6 weeks. An effect of the addition of biochar to the peat on the rhizosphere microbiome was solely measured for the bacterial community from week 6 of plant growth onwards. When scoring the plant development, biochar addition was associated with enhanced root formation, fruit production, and postharvest resistance of the fruits against B. cinerea. We hypothesize that the bacterial rhizosphere microbiome, but also biochar-mediated changes in chemical substrate composition could be involved in these events. Infection of the strawberry leaves with B. cinerea induced shifts in the bacterial rhizosphere community, with an increased bacterial richness. This disease-induced effect was not observed in the rhizospheres of the B. cinerea-infected plants grown in the biochar-amended peat. The results show that an above-ground infection has its effect on the strawberry rhizosphere microbiome, changing the bacterial interactions in the root-substrate interface. This infection effect on the bacterial rhizosphere microbiome seems to be comparable to, but less pronounced than the effect of biochar-addition to the peat. The biological meaning of these observations needs further research, but this study indicates that biochar and an above-ground pathogen attack help the plant to recruit rhizosphere microbes that may aid them in their plant growth and health.

show abstract

Section: Discussionmentioning

confidence: 99%

Dynamics in the Strawberry Rhizosphere Microbiome in Response to Biochar and Botrytis cinerea Leaf Infection

Tender

Haegeman²,

Vandecasteele³

et al. 2016

Front. Microbiol.

View full text Add to dashboard Cite

show abstract

“…While Nocardioides are primarily known for their ability to degrade organic compounds, some strains have recently shown to have antifungal and plant growth-promoting activity ( 85 , 86 ). For example, N. thermolilacinus strain SON-17 can reduce disease severity by inhibiting the germination of spores of pathogenic fungi ( 87 ), while actinomycin produced by N. luteus has shown promising activity against some pathogenic fungi and bacteria ( 88 ). Even though the single Nocardioides sp.…”

Section: Discussionmentioning

confidence: 99%

Phages enhance both phytopathogen density control and rhizosphere microbiome suppressiveness

Wang,

Huang

et al. 2024

mBio

View full text Add to dashboard Cite

Bacteriophages, viruses that specifically target plant pathogenic bacteria, have emerged as a promising alternative to traditional agrochemicals. However, it remains unclear how phages should be applied to achieve efficient pathogen biocontrol and to what extent their efficacy is shaped by indirect interactions with the resident microbiota. Here, we tested if the phage biocontrol efficacy of Ralstonia solanacearum phytopathogenic bacterium can be improved by increasing the phage cocktail application frequency and if the phage efficacy is affected by pathogen-suppressing bacteria already present in the rhizosphere. We find that increasing phage application frequency improves R. solanacearum density control, leading to a clear reduction in bacterial wilt disease in both greenhouse and field experiments with tomato. The high phage application frequency also increased the diversity of resident rhizosphere microbiota and enriched several bacterial taxa that were associated with the reduction in pathogen densities. Interestingly, these taxa often belonged to Actinobacteria known for antibiotics production and soil suppressiveness. To test if they could have had secondary effects on R. solanacearum biocontrol, we isolated Actinobacteria from Nocardia and Streptomyces genera and tested their suppressiveness to the pathogen in vitro and in planta . We found that these taxa could clearly inhibit R. solanacearum growth and constrain bacterial wilt disease, especially when combined with the phage cocktail. Together, our findings unravel an undiscovered benefit of phage therapy, where phages trigger a second line of defense by the pathogen-suppressing bacteria that already exist in resident microbial communities. IMPORTANCE Ralstonia solanacearum is a highly destructive plant-pathogenic bacterium with the ability to cause bacterial wilt in several crucial crop plants. Given the limitations of conventional chemical control methods, the use of bacterial viruses (phages) has been explored as an alternative biological control strategy. In this study, we show that increasing the phage application frequency can improve the density control of R. solanacearum , leading to a significant reduction in bacterial wilt disease. Furthermore, we found that repeated phage application increased the diversity of rhizosphere microbiota and specifically enriched Actinobacterial taxa that showed synergistic pathogen suppression when combined with phages due to resource and interference competition. Together, our study unravels an undiscovered benefit of phages, where phages trigger a second line of defense by the pathogen-suppressing bacteria present in resident microbial communities. Phage therapies could, hence, potentially be tailored according to host microbiota composition to unlock the pre-existing benefits provided by resident microbiota.

show abstract

“…tomato and Xanthomonas campestris pv. vesicatoria, in both laboratory and field trials (Filho et al 2008). Methylorubrum extorquens benefits host development and stress tolerance by altering plant hormone signaling pathways, downregulating senescence and cell death-associated genes, and inducing ononitol biosynthesis in pine trees (Koskimäki et al 2022).…”

Section: Methylobacteriamentioning

confidence: 99%

Tar Spot Disease Severity Influences Phyllosphere-Associated Bacterial and Fungal Microbiomes

Singh,

Crane,

Shim

et al. 2024

Preprint

View full text Add to dashboard Cite

Tar spot, caused by the obligate fungal pathogenPhyllachora maydis, is a foliar disease of corn that has become a major economic concern in the United States. To test the hypothesis thatP. maydiscan interact with other foliar microorganisms, we investigated phyllosphere microbiomes in relation to corn inbreds with differential tar spot symptoms under natural infestation in the field. Leaf samples from sixteen inbred lines were assessed for tar spot symptoms, and bacterial and fungal microbiomes were characterized by paired-end sequencing on the Illumina MiSeq platform. Comparison of the phyllosphere microbiomes revealed distinct bacterial and fungal communities between resistant and susceptible lines. Bacterial and fungal species richness was significantly higher in resistant compared to susceptible inbred lines in a sample-specific manner. In contrast, there were no clear differences in diversity when including evenness of bacterial communities between the resistant and susceptible lines. Diversity of fungal communities differed significantly, particularly between twelve of the fourteen susceptible lines versus resistant lines. Plant-beneficial bacterial genera such asMethylorubrumandQuadrisphaerawere associated with resistant lines, whilePantoea, DeinococcusandPseudomonaswere the least abundant. The second-most commonly detected fungus likely was aConiothyrium, but whether it is the same species that was identified as a possible mycoparasite ofP. maydisin Central and South America is not known. Fungal generaCladosporium, Papiliotrema, Cryptococcus, TilletiopsisandAlternariawere associated with resistant lines whileSphaerellopsiswas the least-abundant genus. In contrast,Puccinia, SphaerellopsisandPhyllachorawere the dominant fungal genera in susceptible lines. Our findings imply thatP. maydisinfection may result in a distinct microbiota with lower diversity. Further analyses of these distinct microbiota between resistant and susceptible lines could lead to a better understanding of the potential role of foliar microbiomes in causing or resistingP. maydisinfection.

show abstract

Biocontrole de doenças de parte aérea do tomateiro por Nocardioides thermolilacinus

Cited by 13 publications

References 6 publications

Dynamics in the Strawberry Rhizosphere Microbiome in Response to Biochar and Botrytis cinerea Leaf Infection

Dynamics in the Strawberry Rhizosphere Microbiome in Response to Biochar and Botrytis cinerea Leaf Infection

Phages enhance both phytopathogen density control and rhizosphere microbiome suppressiveness

Tar Spot Disease Severity Influences Phyllosphere-Associated Bacterial and Fungal Microbiomes

Contact Info

Product

Resources

About