Estudo epidemiológico do HPV na mucosa oral por meio de PCR

Tristão, Willys; Ribeiro, Rodrigo Metzker Pereira; Oliveira, Camila Andréa de; Betiol, Julio Cesar; Bettini, Jussara de Sousa Ribeiro

doi:10.1590/s1808-86942012000400013

Cited by 26 publications

(14 citation statements)

References 10 publications

(12 reference statements)

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“…Entretanto não há a configuração de uma faixa de período de destaque de ocorrência da infecção, também não foi encontrado na literatura dados que associem a infecção do HPV com sazonalidade. A transmissão estaria associada a fatores como: vida sexual precoce, a falta de uso de preservativos, número elevado de parceiros sexuais, gestações, uso de anticoncepcionais, tabagismo e imunodepressão (RAMA et al, 2008;AYRES & SILVA, 2010;TRISTÃO et al, 2012;GIRIANELLI et al, 2014). CONCLUSÃO No estudo foram analisados 10.984 prontuários, onde 2,4% das mulheres possuíam infecção pelo Papilomavírus humano, sendo destacado principalmente em mulheres de 38 a 49 anos, apesar de essa incidência ser mais comum em mulheres mais jovens.…”

Section: Resultsunclassified

“…A taxa de contaminação pelo HPV é maior nos primeiros anos de atividade sexual, entre 18 a 28 anos, e a principal porta de entrada deste vírus é decorrente do não uso de preservativos, número elevado de parceiros sexuais e gestações, uso de anticoncepcionais, tabagismo, vida sexual precoce e imunodepressão (RAMA et al, 2008;AYRES & SILVA, 2010;TRISTÃO et al, 2012).…”

Section: Introductionunclassified

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LEVANTAMENTO DA OCORRENCIA DE HPV (Papilomavírus Humano) EM EXAMES COLPOCITOLÓGICO NO CAIS MULHER – ANÁPOLIS – GO

Sousa¹,

Oliveira²,

Gomes³

et al. 2015

Enciclopédia Biosfera

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RESUMOO papilomavírus humano (HPV) é considerado uma doença sexualmente transmitida com maior prevalência e incidência do mundo. O estudo teve por objetivo investigar a infecção pelo HPV em mulheres que realizaram colpocitológico no CAIS mulher de Anápolis/GO no período de julho de 2012 à julho de 2014. Utilizou-se como recurso metodológico pesquisa documental, descritiva e quantitativa. Dos 10.984 prontuários analisados, 2.4% das mulheres possuíam infecção pelo HPV, destacando principalmente as mulheres de 38 a 49 anos (35%). Não existem dados na literatura que associem a infecção do HPV com sazonalidade. A transmissão estaria associada a fatores como: vida sexual precoce, a falta de uso de preservativos, número elevado de parceiros sexuais, gestações, uso de anticoncepcionais, tabagismo e imunodeprimidos. PALAVRAS-CHAVE: Colpocitológico; infecção; mulheres; papilomavírus humano (HPV); vida sexual SURVEY OF THE OF HPV (Human Papillomavirus) OCCURRENCE WITH PAP SMEAR IN CAIS MULHER -ANÁPOLIS -GOABSTRACT Human papillomavirus (HPV) is considered one of the sexually transmitted diseases with the highest prevalence and incidence in the world. The study aimed to investigate HPV infection in women who had Pap smear in CAIS Mulher from Anapolis/GO from July 2012 to July 2014. It was used as a methodological feature documentary, descriptive and quantitative research. Of the 10.984 records analyzed, 2.4% of women had HPV infection, especially highlighting women 38-49 years (35%). There are no data in the literature linking HPV infection with seasonality. The transmission would be associated with factors such as early sexual life, lack of condom use, high numbers of sexual partners, pregnancies, contraceptive use, smoking and immunocompromised.

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Section: Resultsunclassified

Section: Introductionunclassified

LEVANTAMENTO DA OCORRENCIA DE HPV (Papilomavírus Humano) EM EXAMES COLPOCITOLÓGICO NO CAIS MULHER – ANÁPOLIS – GO

Sousa¹,

Oliveira²,

Gomes³

et al. 2015

Enciclopédia Biosfera

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“…41 Positive results were more clearly seen in samples obtained from heart valves than those from the oral cavity (Fig. 1).…”

Section: Electrophoresismentioning

confidence: 92%

“…The pair of primers taken from the publication of Tristao W. et al were used, with a sequence as follow: GH20 (GAAGAGCCAA GGACAGGTAC) and PC04 (CAACTTCATC CACGTTCACC). 41 The concentration of primers was 10 pmol/μL (Syngen Biotech Poland.) Specific 16S rRNA-based polymerase chain reaction primers were used to detect P. gingivalis, as described by Slots et al with the forward primer 5′AGGCAGCTTG CCATACTGCG, and the reverse primer 5′ACTGTTTAGCA ACTACCGATGT.…”

Section: Dna Extraction Protocolmentioning

confidence: 99%

Porphyromonas gingivalisin periodontal pockets and heart valves

et al. 2014

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“…Th e age of subjects in this study, however, was between 20-31 years and all were university students, which suggests that a higher socioeconomic status may play a role in these fi ndings. In contradiction with these results, another study reported as much as 23.2% of HPVpositive samples from normal oral mucosa (15). A very large study in USA detected HPV DNA in oral rinses in 7.3% of 4846 subjects included (16).…”

mentioning

confidence: 89%

The prevalence of human papillomavirus in patients with oral lichen planus and normal oral mucosa

Radochová¹,

L²,

Slezák³

2015

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Oral lichen planus (OLP) is a chronic infl ammatory disorder that aff ects the oral mucous membrane. Th e prevalence of OLP in the population is considered to be 1%-2% (1). Th e etiology of OLP is not known. Th e immune system very probably plays the primary role in the development of this disease. During the last decades, several studies have suggested that human papillomaviruses (HPVs) are involved in the development of premalignant and malignant lesions (2). Th e causal role of HPV has been reported for OLP and oral squamous cell carcinoma, but there are wide variations in disease prevalence with regard to diff erent geographic populations (3). Th e aim of this study was to determine the prevalence of HPV on the mucosa in patients with OLP and compare it with healthy mucosa.Study protocol has been approved by the local ethical committee. Written informed consent was obtained from all subjects entering the study. Patients with OLP were included based upon following criteria: clinically diagnosed OLP according to objective examination (single physician) and histologically confi rmed OLP. OLP was classifi ed according to criteria previously published by World health Organization and modifi ed by van der Meij et al. (4). Smokers and patients with alcohol abuse history were excluded from the study. Sex-and age-matched controls were chosen from individuals undergoing various dental procedures with no known mucosal disease. Native tissue samples were sent for HPV genome detection. HPV DNA was extracted from native tissue. All samples were screened for the presence of HPV DNA by PCR amplifi cation with primers GP5+/ GP6+ located within the HPV L1 gene. Th e sequences of the forward and reverse primers used were FW (GP5+): 5'-TTTGTTACTGTGGTAGATACTAC-3' and REV (GP6+): 5'-AAAAATAAACTGTAAATCATATT-3'. Th is allows the detection of 37 HPV types, specifi cally 6,11,16, 18, 26, 31, 33, 35, 39, 40, 42, 45, 51, 52, 53, 54, 55, 56, 58, 59, 61, 62, 64, 66, 67, 68, 69, 70, 71, 72, 73 (MM9), 81, 82 (MM4), 83 (MM7), 84 (MM8), IS39, and CP6108. HPV typing was performed, however, due to low amounts of DNA it was not possible to analyze the results. Chi-square and Student t-test were used for comparisons. P value <0.05 was considered statistically signifi cant. A total of 45 patients with confi rmed diagnosis of OLP were examined for HPV, of whom, 33 (73.3%) were woman and 12 (26.7%) were men. Th e mean age of the patients was 56 years (±14.96) with an overall range of 33-77 years. Th e control group consisted of 24 individuals, 19 (79.2%) were women and 5 (20.8%) were men. Th e mean age of the individuals in control group was 56.1 years (±11.41) with an overall range of 29-81 years. Th ere was no statistical signifi cance according to age (p = 0.96) and gender (p = 0.59) between OLP patients and controls. Th ere were 24 (53.3%) HPV-positive patients with OLP and 21 (46.7%) patients were HPV-negative. In the control group there were 12 sub-

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Estudo epidemiológico do HPV na mucosa oral por meio de PCR

Cited by 26 publications

References 10 publications

LEVANTAMENTO DA OCORRENCIA DE HPV (Papilomavírus Humano) EM EXAMES COLPOCITOLÓGICO NO CAIS MULHER – ANÁPOLIS – GO

LEVANTAMENTO DA OCORRENCIA DE HPV (Papilomavírus Humano) EM EXAMES COLPOCITOLÓGICO NO CAIS MULHER – ANÁPOLIS – GO

Porphyromonas gingivalisin periodontal pockets and heart valves

The prevalence of human papillomavirus in patients with oral lichen planus and normal oral mucosa

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