Protective Effects of Ascorbic Acid Pretreatment in a Rat Model of Intestinal Ischemia-Reperfusion Injury: A Histomorphometric Study

Higa, Oscar Haruo; Parra, Edwin Roger; Ab’Saber, Alexandre Muxfeldt; Farhat, Cecília; Higa, Rita; Capelozzi, Vera Luíza

doi:10.1590/s1807-59322007000300017

Cited by 29 publications

(23 citation statements)

References 27 publications

(29 reference statements)

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“…A study using an experimental model in rabbits with vitamin C, mannitol and N-acetylcysteine has been shown that the superior mesenteric artery flow was improved by the three drugs, but only vitamin C and mannitol had direct protective action on gut mucosa at the histopathological exam of the small intestine, with significant reduction of ischemia and reperfusion injury 10 . These results were confirmed by another article that showed morphological improvement of the lesion after administration of ascorbic acid 11 . Another study evaluated the role of non-enzymatic antioxidants using the measurement of levels of malondialdehyde (MDA) and glutathione in the intestinal mucosa, as well as a qualitative histopathologic score.…”

Section: Discussion Discussion Discussion Discussionsupporting

confidence: 61%

Antioxidantes enterais em lesões de isquemia e reperfusão em ratos

Köhler

Delucca

Neto

2011

Rev. Col. Bras. Cir.

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Enteral antioxidants in ischemia/reperfusion injuries in rats Enteral antioxidants in ischemia/reperfusion injuries in rats Enteral antioxidants in ischemia/reperfusion injuries in rats Enteral antioxidants in ischemia/reperfusion injuries in rats Enteral antioxidants in ischemia/reperfusion injuries in rats Antioxidantes enterais em lesões de isquemia e reperfusão em ratos Antioxidantes enterais em lesões de isquemia e reperfusão em ratos Antioxidantes enterais em lesões de isquemia e reperfusão em ratos Antioxidantes enterais em lesões de isquemia e reperfusão em ratos Antioxidantes enterais em lesões de isquemia e reperfusão em ratos HUGO Methods Methods Methods Methods: Ninety adult male Wistar rats were used. An intestinal segment was isolated based on its vascular pedicle. A control biopsy was performed and the pedicle was sectioned and sutured again, ensuring a time of 60 minutes of ischemia followed by reperfusion. Sequential biopsies were performed at the end of the ischemic period and every 15 minutes during reperfusion. The treatment consisted of saline, vitamin C, vitamin E or a combination of the latter two. Quantitative and qualitative assessments of the biopsies were performed. Results Results ResultsResults Results: The groups treated with vitamin E alone or vitamin E combined with vitamin C showed a statistically significant attenuation of ischemia-reperfusion, with reduced loss of height of the villi and lower neutrophilic infiltration at the end of the study when compared to the control and vitamin C- INTRODUCTION INTRODUCTION INTRODUCTION INTRODUCTION INTRODUCTIONT he restoration of blood flow to an ischemic tissue can lead to greater harm than that originally caused by ischemia. This event is called ischemia and reperfusion (I/R) injury. The small intestine is particularly susceptible to injury from I/R 1 and its occurrence is associated with high morbidity and mortality 2 . Although the mechanisms involved are not fully elucidated, it is believed that oxidative stress mediators, such as reactive oxygen species (ROS), polymorphonuclear neutrophils (PMN) and nitric oxide (NO) play an important role 3 . Due to the involvement of ROS in I/R injury, various antioxidants have been tested, among them vitamin C (ascorbic acid) and vitamin E. Ascorbic acid is an water-soluble antioxidant with chelating and reducing properties and it is used to prevent I/R injury 4 . Vitamin E (á-tocopherol) is a nonenzymatic, lipid soluble antioxidant that acts against oxidative stress by stabilizing membrane unsaturated fatty acids. Thus, tissues treated with vitamin E have an increased capacity to reduce ROS and be protected against membrane lipid peroxidation 5 . Vitamins C and E can act synergistically because ascorbic acid is unable to reduce the peroxyl radical, but it can regenerate á-tocopherol from the tocoferoxil radical, recycling the á-tocopherol 6 . I/R can occur in a variety of clinical situations, one being the microsurgical transfer of jejunal segments. The jejunal flap was first described in 1957...

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Section: Discussion Discussion Discussion Discussionsupporting

confidence: 61%

Antioxidantes enterais em lesões de isquemia e reperfusão em ratos

Köhler

Delucca

Neto

2011

Rev. Col. Bras. Cir.

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“…The role of ROS in I/R injury is supported by previous studies which have shown increased PMNLs around the I/R injured areas which release ROS (Yamaguchi et al 1997) and reduced I/R injury secondary to the use of antioxidants such as melatonin, quercetin, ascorbic acid, L-carnitine, n-asetyl cystein, L-arginine and α -tocopherol (Ustundag et al 2000;Mojzis et al 2001;Giakoustidis et al 2006;Chen et al 2007;Dokmeci et al 2007;Higa et al 2007;Sayan et al 2008).…”

mentioning

confidence: 78%

Resveratrol, a Natural Antioxidant, Attenuates Intestinal Ischemia/Reperfusion Injury in Rats

Özkan

Yüzbaşıoğlu

Çıralık

et al. 2009

Tohoku J. Exp. Med.

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“…The occlusion of both mesenteric artery and vein can cause significant injury in the intestine and remote organs and may develop into multiple organ dysfunction syndrome (MODS) 13 .…”

Section: Discussionmentioning

confidence: 99%

Pentoxifylline associated to hypertonic saline solution attenuates inflammatory process and apoptosis after intestinal ischemia/reperfusion in rats

Marques

Rasslan²,

Belon³

et al. 2014

Acta Cir. Bras.

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PURPOSE:To evaluate intestinal inflammatory and apoptotic processes after intestinal ischemia/reperfusion injury, modulated by pentoxifylline and hypertonic saline. METHODS:It was allocated into four groups (n=6), 24 male Wistar rats (200 to 250g) and submitted to intestinal ischemia for 40 min and reperfusion for 80 min: IR (did not receive any treatment); HS group (Hypertonic Saline, 4ml/kg-IV); PTX group (Pentoxifylline, 30mg/ kg-IV); HS+PTX group (Hypertonic Saline and Pentoxifylline). All animals were heparinized (100U/kg). At the end of reperfusion, ileal fragments were removed and stained on hematoxylin-eosin and histochemical studies for COX-2, Bcl-2 and cleaved caspase-3. RESULTS:The values of sO 2 were higher on treated groups at 40 minutes of reperfusion (p=0.0081) and 80 minutes of reperfusion (p=0.0072). Serum lactate values were lower on treated groups after 40 minutes of reperfusion (p=0.0003) and 80 minutes of reperfusion (p=0.0098). Morphologic tissue injuries showed higher grades on IR group versus other groups: HS (p=0.0006), PTX (p=0.0433) and HS+PTX (p=0.0040). The histochemical study showed lesser expression of COX-2 (p=0.0015) and Bcl-2 (p=0.0012) on HS+PTX group. A lower expression of cleaved caspase-3 was demonstrated in PTX (p=0.0090; PTXvsIR). CONCLUSION:The combined use of pentoxifylline and hypertonic saline offers best results on inflammatory and apoptotic inhibitory aspects after intestinal ischemia/reperfusion.

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Protective Effects of Ascorbic Acid Pretreatment in a Rat Model of Intestinal Ischemia-Reperfusion Injury: A Histomorphometric Study

Cited by 29 publications

References 27 publications

Antioxidantes enterais em lesões de isquemia e reperfusão em ratos

Antioxidantes enterais em lesões de isquemia e reperfusão em ratos

Resveratrol, a Natural Antioxidant, Attenuates Intestinal Ischemia/Reperfusion Injury in Rats

Pentoxifylline associated to hypertonic saline solution attenuates inflammatory process and apoptosis after intestinal ischemia/reperfusion in rats

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