Future directions in breeding for disease resistance in aquaculture species

Houston, Ross D.

doi:10.1590/s1806-92902017000600010

Cited by 100 publications

(69 citation statements)

References 61 publications

(66 reference statements)

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“…) within and across populations, decreasing the need to obtain phenotypes for each generation and population (i.e. year‐classes) (Houston ). Combining different sources of information, including GWAs, transcriptomic [e.g.…”

Section: Future Challenges and Opportunitiesmentioning

confidence: 99%

Advances in genetic improvement for salmon and trout aquaculture: the Chilean situation and prospects

Lhorente

Araneda

Neira

et al. 2019

Reviews in Aquaculture

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Genetic improvement is key to the development of a more efficient aquaculture industry. By 2010, there were 104 breeding programmes for aquaculture species in the world, most of them for fish species. Usually, the breeding goals include traits such as growth rate, disease resistance, maturation and carcass quality. Resistance to specific pathogens has been one of the main objectives of research and development in the genetic improvement programmes of salmonids in Chile and worldwide. In Chile, trait selection has been conducted through the application of selection indexes. New technologies like next generation sequencing and genotyping have helped disentangle the genetic basis and enhance genetic evaluation methods of economically important traits. This work aims to review the advances in genetic improvement for salmon and trout aquaculture with emphasis on the Chilean experience. We focus on the implementation of breeding programmes in the country, definition of breeding objectives, results on genetic parameters and response to selection and incorporation of genomics. Future challenges and opportunities regarding genotype‐by‐environment interactions, climate change and research and development, plus the demonstration of the economic benefit of breeding programmes are also addressed.

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Section: Future Challenges and Opportunitiesmentioning

confidence: 99%

Advances in genetic improvement for salmon and trout aquaculture: the Chilean situation and prospects

Lhorente

Araneda

Neira

et al. 2019

Reviews in Aquaculture

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show abstract

“…Genomic selection has also been applied to enhance the rate of genetic gain for disease resistance 53 traits in breeding programmes (Houston 2017;Zenger et al 2019), and genome editing approaches 54 may offer further step-improvements in the future (Gratacap et al, 2019b). However, research into 55 the functional mechanisms underlying host response to salmonid pathogens, and host genetic 56 variation in resistance is important to support development of these potential solutions.…”

Section: Introductionmentioning

confidence: 99%

Efficient genome editing in multiple salmonid cell lines using ribonucleoprotein complexes

Gratacap

Jin

Mantsopoulou

et al. 2020

Preprint

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Infectious and parasitic diseases have major negative economic and animal welfare impacts on aquaculture of salmonid species. Improved knowledge of the functional basis of host response and genetic resistance to these diseases is key to developing preventative and treatment options. Cell lines provide a valuable model to study infectious diseases in salmonids, and genome editing using CRISPR provides an exciting avenue to evaluate the function of specific genes in those systems. While CRISPR/Cas9 has been successfully performed in a Chinook salmon cell line (CHSE-214), there are no reports to date of editing of cell lines derived from the most commercially relevant salmonid species Atlantic salmon and rainbow trout, which are difficult to transduce and therefore edit using lentivirus-mediated methods. In the current study, a method of genome editing of salmonid cell lines using ribonucleoprotein (RNP) complexes was optimised and tested in the most commonly-used salmonid fish cell lines; Atlantic salmon (SHK-1 and ASK cell lines), rainbow trout (RTG-2) and Chinook salmon (CHSE-214). Electroporation of RNP based on either Cas9 or Cas12a was efficient at targeted editing of all the tested lines (typically > 90 % cells edited), and the choice of enzyme expands the number of potential target sites for editing within the genomes of these species. These optimised protocols will facilitate functional genetic studies in salmonid cell lines, which are widely used as model systems for infectious diseases in aquaculture.

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“…However, farmed production of finfish, shellfish, and crustacean species all suffer from infectious diseases that can have negative impacts on animal welfare, the environment, and on commercial viability, constraining future expansion. Selective breeding for improved disease resistance is a promising avenue to tackle these diseases, and has been widely practiced for Atlantic salmon ( Salmo salar ) and other salmonid species (Houston, 2017; Yáñez et al, 2014). Family-based selection based on siblings trait recording is now typically augmented with the use of molecular genetic markers, either via marker-assisted selection (based on markers linked to quantitative trait loci, QTL) or genomic selection using genome-wide markers to predict breeding values (Zenger et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Efficient CRISPR/Cas9 genome editing in a salmonid fish cell line using a lentivirus delivery system

Gratacap

Regan

Dehler

et al. 2019

Preprint

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171 words 14 Main body: 3703 words 15 1Abstract 16 Genome editing is transforming bioscience research, but its application to non-model organisms, such 17 as farmed animal species, requires optimisation. Salmonids are the most important aquaculture 18 species by value, and improving genetic resistance to infectious disease is a major goal. However, 19 use of genome editing to evaluate putative disease resistance genes in cell lines, and the use of 20 genome-wide CRISPR screens is currently limited by a lack of available tools and techniques. In the 21 Lentivirus CRISPR in salmonid cell line 2 current study, an optimised protocol using lentivirus transduction for efficient integration of constructs 22 into the genome of a Chinook salmon (Oncorhynchus tshwaytcha) cell line (CHSE-214) was

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Future directions in breeding for disease resistance in aquaculture species

Cited by 100 publications

References 61 publications

Advances in genetic improvement for salmon and trout aquaculture: the Chilean situation and prospects

Advances in genetic improvement for salmon and trout aquaculture: the Chilean situation and prospects

Efficient genome editing in multiple salmonid cell lines using ribonucleoprotein complexes

Efficient CRISPR/Cas9 genome editing in a salmonid fish cell line using a lentivirus delivery system

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