Comparison of the hemolytic activity between C. albicans and non-albicans Candida species

Rossoni, Rodnei Dennis; Barbosa, Júnia Oliveira; Vilela, Simone Furgeri Godinho; Jorge, Antônio Olavo Cardoso; Junqueira, Juliana Campos

doi:10.1590/s1806-83242013000600007

Cited by 50 publications

(38 citation statements)

References 27 publications

(34 reference statements)

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“…All the isolates analyzed in this study presented a degree of hemolytic activity, and some strains had an HI close to the values obtained for the clinical isolates, corroborating the results reported elsewhere for clinical isolates of this species (Luo et al, 2001; Rossoni et al, 2013; Riceto et al, 2015). On the other hand, most of them had lower hemolysin production than the clinical isolates.…”

Section: Discussionsupporting

confidence: 91%

Evaluation of Virulence Factors In vitro, Resistance to Osmotic Stress and Antifungal Susceptibility of Candida tropicalis Isolated from the Coastal Environment of Northeast Brazil

et al. 2016

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Several studies have been developed regarding human health risks associated with the recreational use of beaches contaminated with domestic sewage. These wastes contain various micro-organisms, including Candida tropicalis. In this context, the objective of this study was to characterize C. tropicalis isolates from the sandy beach of Ponta Negra, Natal, Rio Grande do Norte, Brazil, regarding the expression of in vitro virulence factors, adaptation to osmotic stress and susceptibility to antifungal drugs. We analyzed 62 environmental isolates and observed a great variation among them for the various virulence factors evaluated. In general, environmental isolates were more adherent to human buccal epithelial cells (HBEC) than C. tropicalis ATCC13803 reference strain, and they also showed increased biofilm production. Most of the isolates presented wrinkled phenotypes on Spider medium (34 isolates, 54.8%). The majority of the isolates also showed higher proteinase production than control strains, but low phospholipase activity. In addition, 35 isolates (56.4%) had high hemolytic activity (hemolysis index > 0.55). With regard to C. tropicalis resistance to osmotic stress, 85.4% of the isolates were able to grow in a liquid medium containing 15% sodium chloride. The strains were highly resistant to the azoles tested (fluconazole, voriconazole and itraconazole). Fifteen strains were resistant to the three azoles tested (24.2%). Some strains were also resistant to amphotericin B (14 isolates; 22.6%), while all of them were susceptible for the echinocandins tested, except for a single strain of intermediate susceptibility to micafungin. Our results demonstrate that C. tropicalis isolated from the sand can fully express virulence attributes and showed a high persistence capacity on the coastal environment; in addition of showing high minimal inhibitory concentrations to several antifungal drugs used in current clinical practice, demonstrating that environmental isolates may have pathogenic potential.

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Section: Discussionsupporting

confidence: 91%

Evaluation of Virulence Factors In vitro, Resistance to Osmotic Stress and Antifungal Susceptibility of Candida tropicalis Isolated from the Coastal Environment of Northeast Brazil

et al. 2016

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“…In addition, Rossoni et al . () evaluated the haemolytic activities of C. albi cans and other Candida species, finding that 50 strains of C. albicans and 86% of other Candida strains produced haemolysin. Shuster et al .…”

Section: Discussionmentioning

confidence: 99%

“…A previous study reported the complement-mediated haemolytic activity of C. albicans (Manns et al 1994), andLuo et al (2001) further reported that four Candida species, C. famata, C. guilliermondii, C. rugosa, and C. utilis, showed ahaemolysis. In addition, Rossoni et al (2013) evaluated the haemolytic activities of C. albicans and other Candida species, finding that 50 strains of C. albicans and 86% of other Candida strains produced haemolysin. Shuster et al (2004) determined that not only Candia species but also Saccharomyces species exhibited haemolytic ability under alcohol-mediated conditions.…”

Section: Discussionmentioning

confidence: 99%

Biochemical characteristics, virulence traits and antifungal resistance of two major yeast species isolated from kefir: Kluyveromyces marxianus and Saccharomyces unisporus

Lim

Kim

Jeong

et al. 2019

Int J of Dairy Tech

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Kefir yeasts were investigated for their possible virulence using an in vitro system. Unweighted pair group method with arithmetic mean (UPGMA) cluster analysis of 46 biochemical traits showed that all kefir isolates formed clusters distinct from Candida albicans. No isolate showed N‐acetylglucosaminidase activity, which is involved in resistance against host immune systems. All Kluyveromyces marxianus isolates formed pseudohyphae and grew at 42 °C, whereas Saccharomyces unisporus isolates did not. None of the kefir yeasts had proteolytic or haemolytic activities. All K. marxianus isolates were susceptible to fluconazole, whereas S. unisporus isolates were classified as resistant.

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“…Hemolysin activity enables C. auris assimilate iron from the hemoglobin-heme group [28]. C. albicans, C. dubliniensis, C. glabrata and C. tropicalis also display hemolysin activity [28,29,30,31]. Hemolysin production can be seen as an important virulence factor because it is higher in strains isolated from hospital infections compared to those from environmental sources [1].…”

Section: Production Of Hydrolytic Enzymesmentioning

confidence: 99%

Newly Emerging Candida Specie: Should Clinicians and Mycologist be Concerned?

Ashcroft

Kumurya

Mohammed

et al. 2019

AJRID

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The emergence of C. auris as a global nosocomial pathogen associated with multidrug resistance and high mortality rates has been recently discovered. This emerging pathogen appears to be far more able to induce systemic infection and mortality than other potential multi drug resistance (MDR) yeast pathogens even though it is found to have reduced virulence factors compared to C. albicans. There are issues with regard to the identification of C. auris using both phenotypic and molecular techniques; this has raised concerns about detecting the true scale of the problem. This mini- review elucidates on the literature available on C. auris and highlights the mechanism of pathogenesis and antifungal resistance, which will give further direction to extensive research in this field.

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Comparison of the hemolytic activity between C. albicans and non-albicans Candida species

Cited by 50 publications

References 27 publications

Evaluation of Virulence Factors In vitro, Resistance to Osmotic Stress and Antifungal Susceptibility of Candida tropicalis Isolated from the Coastal Environment of Northeast Brazil

Evaluation of Virulence Factors In vitro, Resistance to Osmotic Stress and Antifungal Susceptibility of Candida tropicalis Isolated from the Coastal Environment of Northeast Brazil

Biochemical characteristics, virulence traits and antifungal resistance of two major yeast species isolated from kefir: Kluyveromyces marxianus and Saccharomyces unisporus

Newly Emerging Candida Specie: Should Clinicians and Mycologist be Concerned?

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