Biofilm biomass disruption by natural substances with potential for endodontic use

Alves, Flávio R.F.; Silva, Marlei Gomes; Rôças, Isabela N.; Siqueira, José F.

doi:10.1590/s1806-83242013000100004

Cited by 28 publications

(16 citation statements)

References 29 publications

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“…The broad‐spectrum antimicrobial activity of LF has been explored in detail to assess its effect on other bacterial species. Alves, Silva, Rôças, and Siqueira () found that application of 0.2% tt‐farnesol, 5% xylitol, and 0.1% LF significantly reduced the biofilm mass formed by Enterococcus faecalis and Staphylococcus epidermidis (bacteria commonly reside in the apical root canal system of teeth with primary or post‐treatment apical periodontitis). This agent has also been used in endodontics as a root canal irrigant to combat pathological biofilms (Alves et al., ).…”

Section: Effect Of Lf On Periodontal Pathogensmentioning

confidence: 99%

Role of lactoferrin and lactoferrin‐derived peptides in oral and maxillofacial diseases

et al. 2018

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The oral cavity harbors different taxonomic groups, the evolutionary coexistence of which develops the oral ecosystem. These resident microorganisms can alter the balance between the physiologic and pathologic conditions that affect the host, both locally and systemically. This highly sophisticated nature of the oral cavity poses a significant therapeutic challenge. Numerous human and animal studies have been conducted to potentiate the efficacy and competence of current treatments of pathologic conditions as well as to develop novel therapeutic modalities. One of these studies is the use of the potent antimicrobial agent lactoferrin (LF), which was originally derived from the host immune system. LF is an 80-kDa glycoprotein that has a free iron sequestration mechanism with evident antimicrobial, anti-tumor, and immunomodulatory properties. A wide range of active peptides have been isolated from the N-terminal region of LF, which possess antimicrobial activities. In this review, we discuss the role of LF and LF-derived peptides under a heterogeneous group of oral and maxillofacial conditions, including bacterial, fungal, viral infections; head and neck cancers; xerostomia; and implantology-bone-related manifestations.

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Section: Effect Of Lf On Periodontal Pathogensmentioning

confidence: 99%

Role of lactoferrin and lactoferrin‐derived peptides in oral and maxillofacial diseases

et al. 2018

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“…Biofilm biomass was visualized and quantified by a crystal violet binding assay as previously described (17,18 Plates were overturned and air-dried, and the dye bound to the adherent cells was solubilized with 150 µL of 95% ethanol for 5 min. For quantification of biofilm biomass remaining after exposure to the test solutions, absorbance of the crystal violet solution was measured using an ELISA reader model 680 (Bio-Rad Laboratories, Hercules, CA, USA) at a wavelength of 590 nm.…”

Section: Crystal Violet Assaymentioning

confidence: 99%

Antibiofilm and Antibacterial Activities of Farnesol and Xylitol as Potential Endodontic Irrigants

et al. 2013

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This study investigated the antibiofilm and antibacterial effects of farnesol and xylitol in a series of experiments in order to evaluate their potential use as root canal irrigants. The following substances were tested: 0.2% farnesol; 5% and 20% xylitol; 0.2% farnesol plus 20% xylitol; and saline (control). For comparison with an established endodontic irrigant, 2.5% NaOCl was included in each test. Three experiments were conducted: the crystal violet assay, to evaluate the effects on the biofilm biomass; the dentin disinfection test, to evaluate the effects on bacterial viability in biofilms; and the root canal disinfection test, to simulate the use in the root canal environment. Farnesol was the most effective substance in reducing the biofilm biomass, followed by 20% xylitol. All substances affected bacterial viability in biofilms; farnesol showed the best results followed by the farnesol/ xylitol combination. Irrigation with all substances significantly reduced the bacterial load (p<0.001), but only the farnesol/xylitol combination was significantly more effective than saline (p=0.02). NaOCl was more effective than any other substance tested in the three experiments (p<0.001). The findings demonstrated that farnesol affected both the biofilm biomass and the viability of cells in the biofilm, while 20% xylitol affected only the biofilm biomass. Although not more effective than NaOCl, the combination of these two antibiofilm substances has potential to be used in endodontics in certain situations.

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“…Although, the poor correlation between the biofilm biomass reduction, metabolic activities and cellular viability remains a major limitation of this method because of the non-selective staining of the biofilm matrix including viable and dead cells as well [53]. Therefore, results obtained from the crystal violet assay must be combined with other multi-parametric techniques such as intracellular ATP, ATP/protein ratio, live/dead cell discrimination and selective visualization of the biomass matrix [54][55][56].…”

Section: Discussionmentioning

confidence: 99%

Cytotoxic Action of Artemisinin and Scopoletin on Planktonic Forms and on Biofilms of Candida Species

et al. 2020

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We investigated the antifungal activities of purified plant metabolites artemisinin (Ar) and scopoletin (Sc) including inhibition, effects on metabolic activities, viability, and oxidative stress on planktonic forms and on preformed biofilms of seven Candida species. The characteristic minimum inhibitory concentration (MIC90) of Ar and Sc against Candida species ranged from 21.83–142.1 µg/mL and 67.22–119.4 µg/mL, respectively. Drug concentrations causing ≈10% CFU decrease within 60 min of treatments were also determined (minimum effective concentration, MEC10) using 100-fold higher CFUs than in the case of MIC90 studies. Cytotoxic effects on planktonic and on mature biofilms of Candida species at MEC10 concentrations were further evaluated with fluorescent live/dead discrimination techniques. Candida glabrata, Candida guilliermondii, and Candida parapsilosis were the species most sensitive to Ar and Sc. Ar and Sc were also found to promote the accumulation of intracellular reactive oxygen species (ROS) by increasing oxidative stress at their respective MEC10 concentrations against the tested planktonic Candida species. Ar and Sc possess dose-dependent antifungal action but the underlying mechanism type (fungistatic and fungicidal) is not clear yet. Our data suggest that Ar and Sc found in herbal plants might have potential usage in the fight against Candida biofilms.

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Biofilm biomass disruption by natural substances with potential for endodontic use

Cited by 28 publications

References 29 publications

Role of lactoferrin and lactoferrin‐derived peptides in oral and maxillofacial diseases

Role of lactoferrin and lactoferrin‐derived peptides in oral and maxillofacial diseases

Antibiofilm and Antibacterial Activities of Farnesol and Xylitol as Potential Endodontic Irrigants

Cytotoxic Action of Artemisinin and Scopoletin on Planktonic Forms and on Biofilms of Candida Species

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