In vitro cytotoxic effects of Brazilian plant extracts on squamous cell carcinoma of the oral cavity

Abstract

In vitro cytotoxic effects of Brazilian plant extracts on squamous cell carcinoma of the oral cavity Abstract: Squamous cell carcinoma (SCC) is the most prevalent cancer of the oral cavity and the fifth most prevalent of all malignancies in males. Many researchers have attempted to develop new treatments that will improve the prognosis of SCC patients. Over 20% of the world's biodiversity is located within the Brazilian forests, but little is known about the chemical and/or pharmacological potential of these plants. Certain extracts obtained from Amazon and Atlantic Forest plants have previously been shown to have cytotoxic activity against various cancers. The aim of this study was to screen these extracts for cytotoxic activity against oral SCC cells. The extracts were analyzed for activity against the KB-ADL#12 cell line at various concentrations up to a maximum dose of 100 µg/mL. Comparisons with a control group were performed using one-way ANOVA. Significant cytotoxicity was induced by the extracts obtained from the aerial parts of Picrolemma sprucei (Simaroubaceae), from the leaves and stems of Laetia suaveolens (Salicaceae), from the aerial parts of Abarema auriculata (Fabaceae-Mimosoideae) and from the stem of A. auriculata.

Analysis of Brazilian Plant Extracts as Potential Source of Antioxidant Natural Products Using Bench-Top Assays

“…The evaluation of the free radical scavenging analyses were made by the establishment of scores as strong (++++), very good (+++), good (++), weak (+) and absence of free radical scavenge activity (0). 16 The following reagents were used in the chemical analysis: 11 (C) Dragendorff 's reagent, (D) 5% KOH diluted in ethanol for checking the presence of anthraquinones; (E) Kedde's reagent.…”

“…7 Our group aimed at the chemical screening of 895 organic and aqueous extracts obtained from plants found in the Amazon rain forest that have been preciously screened for their biological, [8][9][10] pharmacological and toxicological activities. [11][12][13][14][15][16] The extracts were tested for their free radical scavenging and antioxidant activities, as well as screened for the presence of alkaloids, anthraquinones and cardenolides, some of the most active classes of phytochemicals. Table 1; all vouchers are deposited at the Herbarium UNIP.…”

Anti-Streptococcal activity of Brazilian Amazon Rain Forest plant extracts presents potential for preventive strategies against dental caries

“…Much research has been conducted so far, and screening programs have been introduced in many countries, including the United States, Madagascar, Cameroon, Indonesia, China, India, and many others. In Brazil, screening programs have been well established 1,19,27,30 since the Environment Ministry promulgated MP 2.186 in August 26 th , 2001, introducing guidelines to bioprospect the Brazilian biodiversity, which is considered to be the richest in the world and one of the least studied in terms of its pharmacological and chemical potential.…”

In vitro methodological standardization of MTT colorimetric assay for evaluation of formazan activity and drug dosage

“…Aiming to develop essential chemotherapeutic drugs to control various types of cancer, one of the primary tests is the tetrazolium reduction method. This test can determine the minimum inhibitory concentration required to obtain 50% of viable or unviable cells by the cytotoxicity promoted by the tested agent, the time to drug exposure and the efficacy of drug administration [2,3].…”