Evaluation of caries-affected dentin with optical coherence tomography

Azevedo, Cynthia Soares de; Trung, Luciana Cardoso Espejo; Simionato, Maria Regina Lorenzetti; Freitas, Anderson Zanardi de; Matos, Adriana Bona

doi:10.1590/s1806-83242011000500006

Cited by 33 publications

(33 citation statements)

References 22 publications

(27 reference statements)

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“…To estimate the lesion depth on enamel fragments, optical coherence tomography (OCT) was used as previously described [Azevedo et al, 2011]. Human molar impacted teeth were removed, cleaned with pumice paste and water and stored in distilled water at 4 ° C until use.…”

Section: In Vitro Enamel Demineralization Induced By Sugars and Sugarmentioning

confidence: 99%

Sucrose Substitutes Affect the Cariogenic Potential of Streptococcus mutans Biofilms

et al. 2014

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Streptococcus mutans is considered the primary etiologic agent of dental caries and contributes significantly to the virulence of dental plaque, especially in the presence of sucrose. To avoid the role of sucrose on the virulence factors of S. mutans, sugar substitutes are commonly consumed because they lead to lower or no production of acids and interfere with biofilm formation. This study aimed to investigate the contribution of sugar substitutes in the cariogenic potential of S. mutans biofilms. Thus, in the presence of sucrose, glucose, sucralose and sorbitol, the biofilm mass was quantified up to 96 h, the pH of the spent culture media was measured, the expression of biofilm-related genes was determined, and demineralization challenge experiments were conduct in enamel fragments. The presence of sugars or sugar substitutes profoundly affected the expression of spaP, gtfB, gtfC, gbpB, ftf, vicR and vicX in either biofilm or planktonic cells. The substitution of sucrose induced a down-regulation of most genes involved in sucrose-dependent colonization in biofilm cells. When the ratio between the expression of biofilm and planktonic cells was considered, most of those genes were down-regulated in biofilm cells in the presence of sugars and up-regulated in the presence of sugar substitutes. However, sucralose but not sorbitol fulfilled the purpose of reducing the cariogenic potential of the diet since it induced the biofilm formation with the lowest biomass, did not change the pH of the medium and led to the lowest lesion depth in the cariogenic challenge.

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Section: In Vitro Enamel Demineralization Induced By Sugars and Sugarmentioning

confidence: 99%

Sucrose Substitutes Affect the Cariogenic Potential of Streptococcus mutans Biofilms

et al. 2014

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“…It is a high-resolution analysis that enables the visualization beyond the surface, without entering into contact with the tissue of interest. Several studies have used this technology in the dental field to judge on the internal adaptation of fissure sealants, class I cavities, defects inside the mass of composite restorations and degree of demineralization of caries-affected dentin [18][19][20][21][22]. However, no study has assessed the effect of marginal gaps on gap depth, justifying why the present investigation can add interesting information to the existing literature.…”

Section: Introductionmentioning

confidence: 91%

Failure analysis of adhesive restorations with SEM and OCT: from marginal gaps to restoration loss

et al. 2015

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Objective The objective of this study was to analyse the failure mode of adhesive interfaces by comparing OCT and scanning electron microscope (SEM) analysis of class V restoration margins located on enamel and dentin. Materials and methods Three groups were tested that differed in the application of a 3-step etch-and-rinse adhesive system (OptiBond FL) prior to cavity filling with restorative composite resin (Clearfil AP-X). After tooth restoration and polishing, the samples were loaded in a fatigue machine, and adhesive interfaces were evaluated with OCT and SEM. Results Important and complementary information could be obtained with OCT analysis in respect to how marginal defects can propagate inside the cavity, compromising the restoration's long-term performance. A self-etching effect was observed with OptiBond FL due to the presence of an acidic primer (GPDM) within its composition. Our results could show that areas of bonding and gaps coexisted within the same restoration. Conclusions When marginal imperfections, or noncontinuous margins, were detected by SEM, also imperfections beneath the surface could be observed at the toothrestoration interface with OCT. Restoration loss occurred above the borderline of 50 % of marginal gaps on enamel and dentin. Clinical relevance Marginal discrepancies of adhesive restorations can propagate inside the cavity and lead to restoration loss.

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“…Each dentin disc was covered with acid-resistant nail varnish (Colorama, CEIL Coml Exp Ind Ltd., São Paulo, SP, Brazil), except for a 4 × 5 mm area in which caries-affected lesions were produced according to a method described in previously published studies [18,19]. Specimens were sterilized with gamma ray irradiation (25 kGy).…”

Section: Dentin Substratementioning

confidence: 99%

Long-term effect of Er:YAG laser on adhesion to caries-affected dentin

et al. 2018

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Purpose This study investigated the long-term effect of Er:YAG laser pretreatment on the adhesion of a self-etch adhesive system to caries-affected dentin (CAD). Methods Ninety human dentin specimens were submitted to Streptococcus mutans biofilm cariogenic challenge (7 days) to obtain artificial CAD lesions (4 × 5 mm). Groups were defined by the surface pretreatment [no pretreatment; 80 mJ, 2 Hz, 50 μs, Results Three-way ANOVA and Tukey's multiple comparison tests (α = 0.05) showed that CAD had lower μSBS values than SD after 1 year despite surface pretreatments (p = 0.00). AL nanohardness test revealed that specimens' hardness decreased after 1 year (p = 0.00). The HL produced had lower nanohardness in CAD than in SD, which decreased over time for all surface pretreatments (p = 0.00). SEM analysis revealed that all adhesive interfaces, especially in CAD, degraded after 1 year. Conclusions Caries-affected dentin did not seem to be an adequate substrate for bonding. Er:YAG laser irradiation did not influence adhesion to CAD and SD.

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Evaluation of caries-affected dentin with optical coherence tomography

Cited by 33 publications

References 22 publications

Sucrose Substitutes Affect the Cariogenic Potential of <b><i>Streptococcus mutans</i></b> Biofilms

Sucrose Substitutes Affect the Cariogenic Potential of <b><i>Streptococcus mutans</i></b> Biofilms

Failure analysis of adhesive restorations with SEM and OCT: from marginal gaps to restoration loss

Long-term effect of Er:YAG laser on adhesion to caries-affected dentin

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