Influence of bisphosphonates on alveolar bone density: a histomorphometric analysis

Abstract

This study is a histomorphometrical analysis of the influence of the bisphosphonate alendronate on alveolar bone density. Eighteen male Wistar rats were randomly assigned to a control group (n = 9) that received no medication and an experimental group (n = 9) that received oral alendronate (1 mg/kg) from birth until euthanization at 3 months of age. Semi-serial 4-µm-thick transverse sections were obtained from the region between the roots of the left maxillary first molar, stained with hematoxylin and eosin, and examined with a Zeiss Axioskop II optical microscope for histomorphometric analysis. The images were captured with a digital camera coupled with the microscope and connected to a computer, and were analyzed using Image J 1.34s image-analysis software. A 1,200-point grid was positioned onto each digitized image. The number of intersection points of grid lines in the bone tissue was counted. The ratio between the number of points in the bone tissue and the total number of points of the grid (1,200) was used to determine the bone density of the analyzed tissue. Data from the control and experimental groups were compared and analyzed statistically by the Student's t-test (p = 0.05). There was no statistically significant difference (p = 0.3754) in the alveolar bone density between the control and alendronate-treated animals. It may be concluded that the bisphosphonate alendronate did not alter the morphology of the alveolar bone, maintaining its structural tissue characteristics in healthy animals.

Intravenous bisphosphonate therapy does not thicken cementum or change periodontal ligaments of cancer patients

“…5 The real incidence of BRONJ is currently unknown, but was estimated at 4.5-12.8% in patients with multiple myeloma and 1.2-12% patients with metastatic solid carcinomas. 13,[18][19][20][21] In our study, the only patient who developed BRONJ received ZA for prostate cancer metastasis. The present study was unable to assemble a well-standardized sample in terms of time of exposure to BPs, and this limitation was due to the fact that tooth extraction can predispose patients to BRONJ; therefore, it is quite uncommon to have access to a large number of tooth specimens from this patient population.…”

Análisis Histomorfométrico de la Asociación de Aceite de Oliva y Bisfosfonatos en la Remodelación Ósea Periimplantaria

“…La molécula de bifosfonato se deposita entre los cristales de hidroxiapatita, los cuáles serán disueltos por el osteoclasto, procediendo con ello a la endocitosis del bifosfonato. Una vez dentro, la molécula de bifosfonato se deposita en el citosol del osteoclasto, procediendo a su inactivación definitiva (Puel et al, 2006;Santamaria et al, 2010;Veena & Prasad, 2010).…”

A systematic review of microsurgical reconstruction of the jaws using vascularized fibula flap technique in patients with bisphosphonate-related osteonecrosis

“…Specifically, bisphosphonates repress osteoclast-mediated bone remodeling, through disruption of intracellular pathways. As remodeling is a vital role process in tissue renewal and bone healing, bisphosphonate-induced remodeling suppression causes relevant effects on various tissue-level properties 5,7,27,42 .…”