Influence of IL-1 gene polymorphism on the periodontal microbiota of HIV-infected Brazilian individuals

Abstract

This study investigated the association of IL-1A (+4845) and IL-1B (+3954) gene polymorphism with the subgingival microbiota and periodontal status of HIV-infected Brazilian individuals on highly active antiretroviral therapy (HAART). One hundred and five subjects were included in the study, distributed into 2 HIV groups [29 chronic periodontitis (CP+) and 30 periodontally healthy (H+)]; and 2 non-HIV groups (29 CP- and 17 H- patients). IL-1A and B were genotyped by PCR and restriction enzyme digestion. Thirty-three bacterial species were detected by checkerboard. Overall, we observed a prevalence of the allele 2 in the IL1-A and IL-1B polymorphism at 30.5% and 25.7%, respectively. Only 11.4% of all patients were composite genotype-positive, and 75% of those were HIV-infected. No significant associations between polymorphism of the IL-1 gene and periodontitis or HIV infection were observed. Likewise, no significant differences in the frequency and counts of any bacterial species were found between individuals with and without allele 2 (IL-1A or IL-1B). The data indicated that the IL-1 gene polymorphism is neither associated with periodontal destruction nor with high levels of subgingival species, including putative periodontal pathogens in HIV Brazilian individuals on HAART.

Single nucleotide polymorphisms in interleukin-1gene cluster and subgingival colonization with Aggregatibacter actinomycetemcomitans in patients with aggressive periodontitis

“…In HIV-infected Brazilian subjects, no significant association between the composition of subgingival microbiota and genetic variation in IL-1␣ and IL-1␤ could be demonstrated [27]. In contrast, in another clinical study, it was supposed that the individual genetic constellation could influence the composition of subgingival microbiota [27,46]. Carriers of the rare genotypes were shown to be more frequently affected with certain species, primarily those of the red and orange complex.…”

“…The role of A actinomycetemcomitans in the manifestation and progression of aggressive periodontitis in particular is well established [45]. Furthermore, clinical studies were conducted to assessed the genetic impact of IL-1 genes on the subgingival microbiota [27,28,46]. In HIV-infected Brazilian subjects, no significant association between the composition of subgingival microbiota and genetic variation in IL-1␣ and IL-1␤ could be demonstrated [27].…”

“…Furthermore, clinical studies were conducted to assessed the genetic impact of IL-1 genes on the subgingival microbiota [27,28,46]. In HIV-infected Brazilian subjects, no significant association between the composition of subgingival microbiota and genetic variation in IL-1␣ and IL-1␤ could be demonstrated [27]. In contrast, in another clinical study, it was supposed that the individual genetic constellation could influence the composition of subgingival microbiota [27,46].…”

“…On one hand, in a clinical study investigating the impact of SNPs in IL-1␣ and IL-1␤ on the occurrence of oral bacteria, no significant association could be found in human immunodeficiency virus (HIV)-infected patients [27]. By contrast, another clinical study was able to describe characteristic differences in microbial pattern based on IL-1 gene status [28].…”

Interaction of IL1B and IL1RN polymorphisms, smoking habit, gender, and ethnicity with aggressive and chronic periodontitis susceptibility

“…[15] In addition, several studies involved only the IL1B +3954C>T polymorphism or this polymorphism in combination with other polymorphic loci not considered in our work, and in some of them, the control group was not in Hardy–Weinberg equilibrium. [161718]…”