In vitro evaluation of the effect of natural orange juices on dentin morphology

Abstract

The patient's diet has been considered an important etiological factor of dentin hypersensitivity. The frequent ingestion of acidic substances can promote the loss of dental structure or remove the smear layer. The purpose of this study was to evaluate the degree of smear layer removal and dentinal tubules exposure by different natural orange juices. Extracted human teeth were submitted to manual scaling in order to develop the smear layer. Seventy dentin samples were obtained and distributed into the following groups: Control, lime orange, lime, valência orange, navel orange, mandarin, and tangerine. Each group included 2 methods of application: Topical and topical + friction. After preparation for SEM analysis, photomicrographs were assessed by a blind calibrated examiner using an index system. The Kruskal-Wallis test indicated a significant influence of the orange juices on smear layer removal. Significant difference was observed between navel orange, valência orange, mandarin and the control group (p < 0.05). These orange juices resulted in greater removal of the smear layer and greater opening of dentinal tubules. The comparison between the application methods for each group using the Mann-Whitney test showed that friction increased smear layer removal significantly only for lime orange and lime. The data suggest that certain natural orange juices are more effective in terms of smear layer removal and dentinal tubules exposure than others.

Effect of an acid diet allied to sonic toothbrushing on root dentin permeability: an in vitro study

“…These results were also found in other studies (6,13). The acid beverages that caused the highest increase in dentin permeability were lemon juice (187.83%), orange juice (148.33%), vinegar (147.16%), cola drink (139.83%) and white wine (184%).…”

“…The tooth is unique regarding the morphology of dentin tubules, and thus the use of the same teeth as control is necessary (4,11,13). In this study and in previous ones, after acid etching of the teeth, the gauging of the dentin permeability was performed and this value was considered 100%.…”

“…The fluid used was distilled water, which presents a good capacity to permeate dentin. This fluid has previously been used by our research group (13).…”

“…Orange juice (148.33% to 115%), vinegar (147.16% to 95.16%) and lemon juice (187.83% to 147.83%) did not diminish dentin permeability significantly. Zandim et al (13), after application of different orange juices, observed that navel orange, valencia orange and Mandarin orange were more effective in removal of smear layer and exposure of dentin tubules in comparison with lime orange and lime, and the authors showed that toothbrushing without dentifrice tends to increase smear layer removal after orange juice application. This difference probably happened due to the acid features of each beverage.…”

The smear layer created by scaling and root planing is physiologically eliminated in a biphasic process

“…The clinical crowns were separated from the roots with a flexible diamond disc (KG Sorensen), and the root fragments were prepared for microscopic analyses. The samples were dehydrated in increasing grades of ethanol (30%, 50%, 70%, 80%, 95%, and 100%) 20 and dried overnight in a dehydration jar (Corning Life Sciences, São Paulo, Brazil). Finally, the samples were mounted on metallic stubs and sputter-coated with a 25-nm layer of 99.99% pure gold (Balt-Tec SCD-050, Balt-Tec, Kettleshulme, UK).…”