Microscopic qualitative evaluation of fixation time and decalcification media in rat maxillary periodontium

Abstract

The rat model is widely used in periodontal research and the quality of histological sections is essential. The purpose of this study was to evaluate the histological characteristics of periodontal tissues in Wistar rat maxillae, with different times of fixation and decalcified by nitric acid or formic acid (Anna Morse Solution). Fifteen rats were used. Fixation was performed for 24, 48 and 72 hours. The maxillae were hemi-sectioned and each part was decalcified either in nitric acid for 7 days or in Anna Morse solution for 35 days. Two trained and blinded examiners performed the evaluation. Fourty eight hours of fixation and decalcification with Anna Morse solution showed more clear characteristics of the epithelium-connective tissue interface and of the periodontal structures. Mean measurements between the cementum-enamel junction and the bone crest varied in the different experimental times from 176.5 (+/- 60.45) to 210.94 (+/- 39.33) pixels on the buccal aspect, and from 199.69 (+/- 38.33) to 298.55 (+/-70.81) pixels on the palatal aspect, with no statistically significant differences (ANOVA, p > 0.05). In the same fixation period, decalcification with nitric acid or Anna Morse solution did not display any statistically significant differences. It may be concluded that for a qualitative histological analysis, fixation should preferably be for 48 hours and the demineralization should be made by Anna Morse solution. For a histomorphometric analysis, the decalcification solution does not interfere in the results.

Comparison of Different Decalcification Methods Using Rat Mandibles as a Model

“…If not carried out adequately, poor infiltration caused by insufficient tissue processing cycles can impair impregnation of the specimen with paraffin, thus introducing histological artifacts and loss of architectural structure. 9,12 Moreover, structural framework, size, thickness, hardness, and density of tissue can greatly affect rates of decalcification and subsequent procedures. 9,13 To discuss the IHC results, several key tooth development transcription factors must be explained.…”

“…9,12 Moreover, structural framework, size, thickness, hardness, and density of tissue can greatly affect rates of decalcification and subsequent procedures. 9,13 To discuss the IHC results, several key tooth development transcription factors must be explained. Tooth development is an intricate process that is regulated by specific transcription factors.…”

Tibiotarsus bone characteristics and tibial dyschondroplasia incidence of broilers fed diets supplemented with leucine and valine

“…Subsequently, bone concentrations of calcium, phosphorus and ash were determined according to the procedure described by Silva and Queiróz (). The right tibiotarsus was decalcified (Fernandes, Gaio, Rosing, Opperman, & Rado, ) for epiphyseal disc zone observation and characterization of tibial dyschondroplasia lesions according to the procedure proposed by Oviedo‐Rondón, Murakami, Furlan, Moreira, and Macari (). After decalcification, the bone was subjected to histological routine for inclusion in paraffin.…”

Efektivitas Larutan Dekalsifikasi pada Os tibia Domba Garut (Ovis aries) (THE EFFECTIVENESS OF DECALCIFYING SOLUTIONS ON THE TIBIAL OF GARUT SHEEP (OVIS ARIES))

“…Berbagai larutan kimia telah digunakan untuk proses dekalsifikasi, antara lain larutan asam (asam kuat dan asam lemah), agen dekalsifikasi seperti etylene diamenotetraacetic acid (EDTA), dan larutan modifikasi seperti TBD-1 ® yang mengandung EDTA dan asam klorida (HCL) (Fernandes et al, 2007;Lee et al, 2008;Savi et al, 2017). Asam kuat yang umum digunakan untuk dekalsifikasi tulang adalah asam klorida (HCL) dan asam nitrat (HNO 3 ) dengan konsentrasi 5-10%.…”