Viral coinfection in the oral cavity of HIV-infected children: relation among HIV viral load, CD4+T lymphocyte count and detection of EBV, CMV and HSV

Grando, Liliane Janete; Spitzer, Silvia; Nachman, Sharon; Ferguson, Fred; Berentsen, Barbara J.; Yurgel, Liliane Soares

doi:10.1590/s1806-83242005000300013

Cited by 14 publications

(25 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…One of the Brazilian public health priorities is to make HIV medications available free of charge to all HIV-seropositive patients 33 . The consequences of universal access to HAART include a reduction in the number of deaths by AIDS and a reduction of the incidence of opportunistic diseases 34 . Therefore, the present data, in conjunction with other reports, corroborate the hypothesis that HAART has little effect on HHV shedding in saliva, because all HHVs tested in this study were frequently detected in HIV-infected patients.…”

Section: Ethicalmentioning

confidence: 99%

PCR detection of multiple human herpesvirus DNA in saliva from HIV-infected individuals in Teresina, State of Piauí, Brazil

Carvalho

Silvestre

Maciel

et al. 2010

Rev. Soc. Bras. Med. Trop.

View full text Add to dashboard Cite

Introduction: Human herpesviruses are frequently associated with orofacial diseases in humans (HSV-1, EBV, CMV and HHV-8), some can also cause systemic disease . The transmission of these viruses occurs by contact with infected secretions, especially saliva. Human immunodeficiency virus infection is associated with an increased risk of HHVs and related diseases. Methods: This work aimed to detect HSV-1, EBV, CMV and HHV-8 DNA in saliva of HIV-infected patients from Teresina, northeast Brazil, by PCR and compare these findings with age and sex matched HIV-seronegative individuals. Results: No difference in prevalence was verified between HHV detection in the saliva of HIV-seropositive individuals and controls. The individual frequencies of these viruses in these two populations were different. HIV seropositivity correlated positively with the presence of CMV (OR: 18.2, p= 0.00032) and EBV (OR: 3.44, p= 0.0081). No association between CD4 counts and the prevalence of HHVs in the saliva was observed; however, a strong association was determined between seropositivity and the presence of multiple HHV DNAs in saliva (OR: 4.83, p = 0.0028). Conclusions: These findings suggest the asymptomatic salivary shedding of HHVs is a common event between HIV-seropositive and seronegative individuals from Teresina, Piauí, Brazil, and, especially for HIV-seropositive patients, saliva is a risk factor for the acquisition/transmission of multiple HHVs. Key-words:Human herpesvirus. Saliva. PCR. HIV seropositive. RESUMOIntrodução: Alguns herpesvírus humanos são frequentemente associados a doenças orofaciais em humanos. A transmissão destes vírus ocorre através do contato com secreções contaminadas, especialmente a saliva. A infecção pelo vírus da imunodeficiência humana é considerada um fator de risco para a aquisição de HHVs e doenças correlatas. Métodos: Este trabalho teve como objetivo detectar por PCR o DNA de HSV-1, EBV, CMV e HHV-8 na saliva de pacientes infectados com HIV em Teresina, nordeste do Brasil, e comparar os dados obtidos com o grupo controle (indivíduos HIV negativos). Resultados: Não há diferença na prevalência de detecção de HHVs na saliva de indivíduos HIV soropositivos e soronegativos. No entanto, as frequências individuais de detecção dos diferentes HHVs são diferentes entre estas duas populações. A soropositividade para HIV apresentou correlação positiva com a presença de CMV (OR: 18,2, p = 0,00032) e EBV (OR: 3,44, p = 0,0081). Não foi verificada nenhuma associação entre a contagem de CD4 e prevalência de HHVs na saliva, no entanto existe uma forte associação entre a soropositividade e a detecção do DNA de vários HHVs na saliva (OR: 4,83, p = 0,0028). Conclusões: Estes resultados sugerem que a transmissão salivar de HHVs é um evento comum entre os indivíduos HIV soropositivos e soronegativos de Teresina, Piauí, Brasil, e, especialmente para os pacientes soropositivos, a saliva é um fator de risco para a aquisição/transmissão de múltiplos HHVs.Palavras-chaves: Herpesvirus humanos. Saliva. PCR. HIV sorop...

show abstract

Section: Ethicalmentioning

confidence: 99%

PCR detection of multiple human herpesvirus DNA in saliva from HIV-infected individuals in Teresina, State of Piauí, Brazil

Carvalho

Silvestre

Maciel

et al. 2010

Rev. Soc. Bras. Med. Trop.

View full text Add to dashboard Cite

show abstract

“…Infection by CMV was found in 105 (58.3%) children, EBV infection in 177 (98.3%) children and HSV in 56 (31.1%). HIV-infected children with associated viruses had favorable CD4+ T lymphocyte counts and unfavorable viral loads 2 . Compared with our study we found less prevalence of coinfection by HSV, EBV and CMV and a higher prevalence of HHV-7 and HHV-6.…”

Section: Discussionmentioning

confidence: 99%

“…Our results were in accordance with the literature 20,[22][23][24][25][26][27] . However, Grando et al described the EBV prevalence as being 56.43% in Brazilian children and 27.1% in the US children 2 . In a study of Brazilian children, clinical and subclinical OHL was identified in 1.7% and 16.7% of the cohort, respectively.…”

Section: Discussionmentioning

confidence: 99%

“…Human immunodeficiency virus -infected children and adults are prone to opportunistic viral infections in the oral mucosa, mainly from the Herpesviridae family members such as herpes simplex virus (HSV), the cytomegalovirus (CMV) and Epstein-Barr virus (EBV), all of which are important etiologic agents of morbidity [2][3][4] . This family of infectious agents is composed of three groups of viruses and has a distinct characteristic of being able to persist in latency and they depend on some stimulus for their reactivation, as occurs in cases of immunosuppression promoted by HIV infection.…”

Section: Introductionmentioning

confidence: 99%

“…The human herpesvirus 3 (Varicella Zoster Virus -VZV) causes primary varicella, mainly in children and reinfection may be the cause of the herpes zoster (HZ). The human herpesvirus 4 (EBV) is associated with infectious mononucleosis, Burkitt's lymphoma and nasopharyngeal carcinoma [1][2][3][4][5][6] . The human herpes virus 5 (CMV) can cause pneumonia, rhinitis and severe hepatitis, particularly in individuals who have blood transfusions.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Current status of herpesvirus identification in the oral cavity of HIV-infected children

Pinheiro

Ferreira

Costa

et al. 2013

Rev. Soc. Bras. Med. Trop.

View full text Add to dashboard Cite

Introduction: Some viruses of the Herpesviridae family are frequently the etiologic agents of oral lesions associated with HIV. The aim of this study was to identify the presence of herpes simplex virus types 1 and 2 (HSV-1, HSV-2), Varicella Zoster virus (VZV), Epstein-Barr virus (EBV), human cytomegalovirus (HCMV), human herpesvirus type 6, type 7 and type 8 (HHV-6, HHV-7 and HHV-8) in the oral cavity of HIV-infected children/adolescents and verify the association between viral subtypes and clinical factors. Methods: The cells of oral mucosa were collected from 50 HIV infected children/adolescents, 3-13 years old (mean age 8.66). The majority (66%) of selected were girls, and they were all outpatients at the pediatric AIDS clinic of a public hospital in Rio de Janeiro. Nested-PCR was used to identify the viral types. Results: Absence of immunosuppression was observed in 66% of the children. Highly active antiretroviral therapy (HAART) was used by 72.1% of selected and moderate viral load was observed in 56% of the children/adolescents. Viral types were found in 86% of the children and the subtypes were: HSV-1 (4%), HSV-2 (2%), VZV (4%), EBV (0%), HCMV (24%), HHV6 (18%), HHV-7 (68%), HHV8 (0%). Conclusions: The use of HAART has helped to reduce oral lesions, especially with herpes virus infections. The health professionals who work with these patients should be aware of such lesions because of their predictive value and the herpes virus can be found circulating in the oral cavity without causing lesions.

show abstract

A correlation analysis of HHV infection and its predictive factors in an HIV‐seropositive population in Yunnan, China

Ren

Wang

Miao

et al. 2019

Journal of Medical Virology

View full text Add to dashboard Cite

Human herpesviruses (HHVs) have a particularly high prevalence in certain high-risk populations and cause increased morbidity and mortality in patients with acquired immunodeficiency syndrome (AIDS). Screening and treating subclinical HHV infections reduce human immunodeficiency virus (HIV) infection incidence, disease progression, and transmission. However, there are few studies on HHVs, HIV coinfection rates, and their related risk factors. We aimed to clarify the prevalence of all eight HHVs in peripheral blood samples collected from HIV-positive patients, and explore the association of HHV infection in HIV-positive patients in an HIVseropositive population in Yunnan. We recruited 121 HIV-positive patients with highly active antiretroviral therapy (HAART) and 45 healthy individuals. All the eight HHVs were detected using polymerase chain reaction and their epidemiological information and clinical data were collected and statistically analyzed. A high prevalence of HHVs (89.3%) was observed in individuals with HIV infections and with herpes simplex virus (HSV)-2 (65.3%), and HSV-1 (59.5%) being the most common.Coinfection with more than two different HHVs was more common in patients with HIV infections receiving HAART (72.7%) than in healthy controls. Older age, being married, higher HIV-1 plasma viral loads, and use of antiviral protease inhibitors were independently correlated with an increased frequency of HHVs, but we found no association with CD4 count, WHO HIV clinical stage, and HIV infection duration. Our findings are of great significance for the prevention of HHV opportunistic infection in patients with AIDS and their clinical treatment.

show abstract

Viral coinfection in the oral cavity of HIV-infected children: relation among HIV viral load, CD4+T lymphocyte count and detection of EBV, CMV and HSV

Cited by 14 publications

References 18 publications

PCR detection of multiple human herpesvirus DNA in saliva from HIV-infected individuals in Teresina, State of Piauí, Brazil

PCR detection of multiple human herpesvirus DNA in saliva from HIV-infected individuals in Teresina, State of Piauí, Brazil

Current status of herpesvirus identification in the oral cavity of HIV-infected children

A correlation analysis of HHV infection and its predictive factors in an HIV‐seropositive population in Yunnan, China

Contact Info

Product

Resources

About