Diagnóstico imunológico da tuberculose: problemas e estratégias para o sucesso

Teixeira, Henrique Couto; Abramo, Clarice; Munk, Martin E.

doi:10.1590/s1806-37132007000300015

Cited by 44 publications

(22 citation statements)

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“…[43]. No statistical difference, however, was found between this result and TST sensitivity (70–75%) in adults living in endemic countries such as Brazil [18]. However, the specificity of this test was quite high, around 90%, thereby corroborating the results obtained by a number of authors [15, 26, 39, 40, 43, 44] and suggesting that this method may be useful for the diagnosis of TB disease in children.…”

Section: Discussionsupporting

confidence: 77%

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Immunological Diagnosis of Tuberculosis Based on Recombinant Antigens ESAT-6 and CFP-10 in Children from an Endemic Area in Northeast Brazil

Van-Lume¹,

Souza²,

Cabral

et al. 2010

Scandinavian Journal of Immunology

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Diagnostic tests for tuberculosis (TB) using interferon gamma (IFN‐γ) responses produced by T lymphocytes after stimulation by early secretory antigen target 6 (ESAT‐6), culture filtrate protein 10 (CFP‐10) or purified protein derivate (PPD) were carried out using ELISA (enzyme‐linked immunosorbent assay) in whole blood culture supernatants from children with suspected TB disease (n = 21), latent TB infection (LTBI; n = 17) and negative controls (NC; n = 21) from Recife, Pernambuco, Brazil. The results were analysed using the ROC (receiver operating characteristic) curves and the areas under the curve (AUC) generated varied from 0.5 to 1.0 with higher values indicating increased discriminatory ability. Comparisons of AUCs were made using non‐parametric assumptions, and the differences were considered significant if P < 0.05. The ROC curve showed a statistical difference (P = 0.015) between the LTBI and NC groups with an AUC of 0.731, TB disease and NC (AUC = 0.780; P = 0.002) and a group with TB (latent infection + disease, n = 38) and NC (AUC = 0.758; P = 0.001) when the antigen used was ESAT‐6. No statistical difference was found between the groups when CFP‐10 or PPD was used. In conclusion, the ESAT‐6 test may be the most appropriate for diagnosis of childhood TB, both LTBI and TB disease, when associated with epidemiological and clinical data, especially in endemic areas such as Brazil.

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Section: Discussionsupporting

confidence: 77%

“…New strategies for the specific diagnosis of LTBI and TB disease in children are thus urgently needed to overcome the limitations of PPD [15,17,18].…”

Section: Introductionmentioning

confidence: 99%

Immunological Diagnosis of Tuberculosis Based on Recombinant Antigens ESAT-6 and CFP-10 in Children from an Endemic Area in Northeast Brazil

Van-Lume¹,

Souza²,

Cabral

et al. 2010

Scandinavian Journal of Immunology

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“…Around 30-50% of TB patients are negative in the microscopy examination, and culture requires a long time for the growth of M. tuberculosis , which maybe lead to delay diagnosis [4,5]. The tuberculin skin test has long been used for the diagnosis of TB.…”

Section: Introductionmentioning

confidence: 99%

IgG, IgM and IgA antibodies against the novel polyprotein in active tuberculosis

Xia¹,

Yang²,

Xiu³

et al. 2014

BMC Infect Dis

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BackgroundThe present study was aimed to evaluate whether IgG, IgM and IgA antibodies levels detected against a novel Mycobacterium tuberculosis polyprotein 38 F-64 F (with 38 F being the abbreviation for 38kD-ESAT6-CFP10 and 64 F for Mtb8.4-MPT64-TB16.3-Mtb8) are suitable for diagnosing active tuberculosis, and for monitoring the efficacy of chemotherapy on TB patients.MethodsIn this study, a total of 371 active TB patients without treatment were selected and categorized into S+/C+ group (n = 143), S-/C+ group (n = 106) or S-/C- group (n = 122). A series of serum samples were collected from 82 active TB patients who had undergone anti-TB chemotherapy for 0–6 months at one month interval. Humoral responses (IgG, IgM and IgA) were determined for the novel Mycobacterium tuberculosis polyprotein using indirect ELISA methods in all of serum samples.ResultsFor S+/C+, S-/C+ and S-/C- active tuberculosis patients before anti-TB chemotherapy, the sensitivities of tests based on IgG were 65.7%, 46.2% and 52.5% respectively; the sensitivities based on IgM were 21.7%, 24.5% and 18.9%; and the sensitivities based on IgA were 25.2%, 17.9% and 23.8%. By combination of three isotypes, for all active tuberculosis patients, the test sensitivity increased to 70.4% with the specificity being 91.5%. After anti-TB chemotherapy, there were no significant differences between groups with different courses of anti-TB chemotherapy.ConclusionsThe novel Mycobacterium tuberculosis polyprotein 38 F-64 F represents potential antigen suitable for measuring IgG, IgM and IgA antibodies. However, the serodiagnostic test based on the 38 F-64 F polyprotein appears unsuitable for monitoring the efficacy of chemotherapy.

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“…Many attempts to develop an improved serological assay for TB have used this antigen [30, 76]. Early studies reported 89% sensitivity and 100% specificity in TBA patients [31].…”

Section: Human Tuberculosismentioning

confidence: 99%

Opportunities for Improved Serodiagnosis of Human Tuberculosis, Bovine Tuberculosis, and Paratuberculosis

Wadhwa

Hickling

Eda

2012

Veterinary Medicine International

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Mycobacterial infections—tuberculosis (TB), bovine tuberculosis (bTB), and Johne's disease (JD)—are major infectious diseases of both human and animals. Methods presently in use for diagnosis of mycobacterial infections include bacterial culture, nucleic acid amplification, tuberculin skin test, interferon-γ assay, and serology. Serological tests have several advantages over other methods, including short turn-around time, relatively simple procedures, and low cost. However, current serodiagnostic methods for TB, bTB and JD exhibit low sensitivity and/or specificity. Recent studies that have aimed to develop improved serodiagnostic tests have mostly focused on identifying useful species-specific protein antigens. A review of recent attempts to improve diagnostic test performance indicates that the use of multiple antigens can improve the accuracy of serodiagnosis of these mycobacterial diseases. Mycobacteria also produce a variety of species-specific nonprotein molecules; however, only a few such molecules (e.g., cord factor and lipoarabinomannan) have so far been evaluated for their effectiveness as diagnostic antigens. For TB and bTB, there has been recent progress in developing laboratory-free diagnostic methods. New technologies such as microfluidics and “Lab-on-Chip” are examples of promising new technologies that can underpin development of laboratory-free diagnostic devices for these mycobacterial infections.

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Diagnóstico imunológico da tuberculose: problemas e estratégias para o sucesso

Cited by 44 publications

References 50 publications

Immunological Diagnosis of Tuberculosis Based on Recombinant Antigens ESAT-6 and CFP-10 in Children from an Endemic Area in Northeast Brazil

Immunological Diagnosis of Tuberculosis Based on Recombinant Antigens ESAT-6 and CFP-10 in Children from an Endemic Area in Northeast Brazil

IgG, IgM and IgA antibodies against the novel polyprotein in active tuberculosis

Opportunities for Improved Serodiagnosis of Human Tuberculosis, Bovine Tuberculosis, and Paratuberculosis

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