Padronização de modelo experimental adequado a estudos do efeito do exercício na artrite

Abstract

Objective:To standardize an experimental model of chronic monoarthritis induced by complete Freund's adjuvant appropriate for the analysis of the effect of walking on nociception and on joint edema.Methods:The following factors were evaluated as to monoarthritis induction: route and site of administration, number and interval of inoculations, Mycobacterium species, and animal gender. Wistar male and female rats (200 to 250g) received two injections of complete Freund's adjuvant containing Mycobacterium tuberculosis (1.0mg/mL; 50μL) or Mycobacterium butyricum (0.5mg/mL; 50μL) intra-articularly in the tibiotarsal or tibiofemoral joints, or an injection of complete Freund's adjuvant (Mycobacterium butyricum or tuberculosis) intradermally at the base of the tail and another intra-articularly (tibiotarsal or tibiofemoral). The animals were submitted to evaluations of articular disability and edema. Articular disability was assessed by paw elevation time (in seconds) during the one-minute walk test. Edema of the tibiofemoral joint was assessed by variation of joint diameter (cm). Tibiotarsal joint edema was measured by the volume of the paw (mL).Results:Administration of complete Freund's adjuvant containing Mycobacterium butyricum increased paw elevation time and edema in both joints.Conclusion:These data allow standardization of an animal model of chronic monoarthritis adequate for analysis of the effects of exercise on treatment of rheumatoid arthritis.

Anti-inflammatory and Anti-arthritic Activities of Glycosylated Flavonoids from Syzygium jambos in Edematogenic Agent-Induced Paw Edema in Mice

“…In the first phase, edema rate of the injected footpad increased and reached a peak during the first 3 days. Thereafter, the swelling slowly subsided until the seventh day when the paw began to swell again and peaked in the second week (second phase); this effect of increasing edema (increase in inflammatory mediators) after 7 days of CFA injection has already been reported by other researchers (Gomes et al 2013). The administration of the aqueous fraction (5 mg/kg) inhibited the development of joint swelling induced by CFA (51.39%) when compared to the 5 mg/kg IND-positive control (63.19%) on the eighth day after CFA injection (Fig.…”

In vivo anti-arthritic and anti-nociceptive effects of ethanol extract of Moringa oleifera leaves on complete Freund's adjuvant (CFA)-induced arthritis in rats

“… 2 , 3 RA may possibly progress to severe disability with direct negative impacts on life style and increase in mortality rate. 4 , 5 The overall prevalence of clinically diagnosed RA was 0.5–2% of the population with higher prevalence in developed countries. 6 All ages are susceptible to develop RA, but the incidence increased significantly in people aged over 40 years, especially women who are two to three times more susceptible to RA than men.…”

Resistance exercise and low‐level laser therapy improves grip strength and morphological aspects in the ankle joint of Wistar rats with experimental arthritis

“…of motion, and flexibility, decrease pain, improve gait (Kapale et al, 2017;Shih et al, 2006), increase muscle mass, reduce adiposity, and improve muscle strength, physical fitness, fatigue, psychological well-being, and overall life quality (Mcinnes & Schett, 2011). Gomes et al (2013) reported that physical exercise could reduce edema and leukocyte migration in animals with CFA-induced arthritis. Evidence suggests that activity can be performed as soon as the RA diagnosis with no joint damage is confirmed.…”