Comparative study of the results from conventional cervico-vaginal oncotic cytology and liquid-based cytology

Stabile, Sueli Aparecida Batista; Evangelista, Dilson Henrique Ramos; Talamonte, Valdely Helena; Lippi, Umberto Gazi; Lopes, Reginaldo Guedes Coelho

doi:10.1590/s1679-45082012000400013

Cited by 16 publications

(17 citation statements)

References 22 publications

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“…A number of non-FDA-approved methods have been developed for use outside of the USA. These include LiquiPrep [4], Turbitec [5], PapSpin [6], TACAS [7,8], Siriraj-LBC [9], DNA-Citoliq (Digene) [10], GluCyte [11], and some other devices [12,13]. Specimen materials collected in the preservation medium are routinely used for cell blocks, which can provide additional diagnostic information, including architectural pattern, immunocytochemistry (ICC) and special stains.…”

Section: Introductionmentioning

confidence: 99%

Overview of Nongynecological Samples Prepared with Liquid-Based Cytology Medium

Ren

Solomides²,

Draganova‐Tacheva³

et al. 2014

Acta Cytologica

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Objective: Liquid-based cytology of nongynecological specimens is commonly used in cytology laboratories throughout the world and various processing methods, such as ThinPrep and SurePath, have been reported. The cytological features and performance of liquid-based cytology for various cytology specimens, including body cavity fluids, urine, brushing specimens and fine-needle aspiration of various lesions, were reviewed and compared with the experience of our laboratory and the literature published in PubMed. Study Design: The parameters for the evaluation of liquid-based cytology and conventional smears were described in the various types of specimens. Criteria for the interpretation of nongynecological liquid-based cytology were highlighted to show differences in cell morphology, background and artifacts. Results: The interpretation requires familiarity with the appearance of liquid-based cytology in the various types of preparations to avoid misdiagnosis. Conclusions: Cell blocks can be prepared with specimens preserved in a liquid-based cytology medium and immunocytochemical stains and molecular testing can be successfully performed. These are important adjuncts in order to reach a definitive diagnosis.

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Section: Introductionmentioning

confidence: 99%

Overview of Nongynecological Samples Prepared with Liquid-Based Cytology Medium

Ren

Solomides²,

Draganova‐Tacheva³

et al. 2014

Acta Cytologica

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“…Several authors have demonstrated and confirmed the advantages of ThinPrep in relation to CP for cervical exams ( 8 , 22 - 25 ). ThinPrep has been referred to as the method of superior performance because it provides better cellular representation, with increased sensitivity for the detection of lesions, compared with conventional methodology ( 5 , 8 , 14 , 24 - 28 ). Several authors have shown that this greater diagnostic sensitivity applies to high-grade and glandular lesions ( 26 - 28 ).…”

Section: Discussionmentioning

confidence: 90%

“…Approved in 1996 by the United States Food and Drug Administration, ThinPrep was introduced as an alternative to using the conventional method, with the purpose of improving the screening of atypical cells, cervical cancer, or its precursor lesions [low-grade squamous intraepithelial lesions (LSIL) and high-grade squamous intraepithelial lesions (HSIL)]. The aim was to improve sensitivity, because it permits the use of a monolayer of cells to facilitate the diagnosis by the cytopathologist, with better cellular preservation and the possibility to carry out molecular biology testing, making possible, for example, HPV and Chlamydia trachomatis DNA detection ( 4 , 8 - 12 ). This technique has been widely adopted and is gradually replacing the CP in control programs of cervical cancer in some countries ( 13 , 14 ).…”

Section: Introductionmentioning

confidence: 99%

Comparison of conventional Papanicolaou cytology samples with liquid-based cervical cytology samples from women in Pernambuco, Brazil

Costa

Heráclio

Coelho

et al. 2015

Braz J Med Biol Res

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In the present study, we compared the performance of a ThinPrep cytological method with the conventional Papanicolaou test for diagnosis of cytopathological changes, with regard to unsatisfactory results achieved at the Central Public Health Laboratory of the State of Pernambuco. A population-based, cross-sectional study was performed with women aged 18 to 65 years, who spontaneously sought gynecological services in Public Health Units in the State of Pernambuco, Northeast Brazil, between April and November 2011. All patients in the study were given a standardized questionnaire on sociodemographics, sexual characteristics, reproductive practices, and habits. A total of 525 patients were assessed by the two methods (11.05% were under the age of 25 years, 30.86% were single, 4.4% had had more than 5 sexual partners, 44% were not using contraception, 38.85% were users of alcohol, 24.38% were smokers, 3.24% had consumed drugs previously, 42.01% had gynecological complaints, and 12.19% had an early history of sexually transmitted diseases). The two methods showed poor correlation (k=0.19; 95%CI=0.11–0.26; P<0.001). The ThinPrep method reduced the rate of unsatisfactory results from 4.38% to 1.71% (χ2=5.28; P=0.02), and the number of cytopathological changes diagnosed increased from 2.47% to 3.04%. This study confirmed that adopting the ThinPrep method for diagnosis of cervical cytological samples was an improvement over the conventional method. Furthermore, this method may reduce possible losses from cytological resampling and reduce obstacles to patient follow-up, improving the quality of the public health system in the State of Pernambuco, Northeast Brazil.

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“…The majority of subjects had healthy cervix and vagina. Totally, 35 (95%) women had postmenopausal changes on per speculum examination, like cervix flushed with vagina (19), narrowing of vagina (12), and senile vaginitis (10). It was observed that Pap smear reported 14.3% inadequate smears in women with postmenopausal changes and 5% inadequate smears in women without postmenopausal changes.…”

Section: Resultsmentioning

confidence: 99%

Liquid-based Cytology vs Conventional Cytology as a Screening Tool for Cervical Cancer in Postmenopausal Women

Singh¹,

Singh²,

Qureshi³

et al. 2017

Journal of South Asian Federation of Obstetrics and Gynaecology

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Aim: To evaluate and compare the efficacy of liquid-based cytology (LBC) with conventional cytology (CS) as a screening tool for cervical cancer in postmenopausal women. Materials and methods:This was an observational crosssectional study conducted over one year. Subjects were recruited from patients attending the outpatient department of the Department of Obstetrics and Gynecology, King George's Medical University, Lucknow, Uttar Pradesh, India. Pap smear and LBC were taken in all subjects. In cases with suspected lesions, the discrepancy between Pap smear and LBC and colposcopy was found.Results: Pap smear reported 14.3% inadequate smears in women with postmenopausal changes and 5% inadequate smears in women without postmenopausal changes. In contrast, LBC reported no inadequate smear. This difference was statistically significant (p = 0.011). Cervical neoplasia was ruled out in 53.6% cases by Pap smear and 75.8% cases by LBC. The difference was statistically significant (p = 0.002). Conclusion:Liquid-based cytology offers better results in detecting cervical pathology when compared with Pap smear. Liquid-based cytology could be better as a screening method, especially in postmenopausal women in which the screening is challenging due to menopause-induced anatomical and hormonal changes.Clinical significance: Cervical cancer is the most common genital cancer among Indian women. Postmenopausal women are at higher risk for developing cervical cancer. Low estrogenic state poses a challenge for detection of cervical cancer by CS in postmenopausal women. We investigated LBC as a better cervical cancer screening tool in this age group.

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Comparative study of the results from conventional cervico-vaginal oncotic cytology and liquid-based cytology

Abstract: Liquid-based cytology had a better performance to diagnose atypical cells and the cytohistological concordance was higher than in the conventional cytology.

Cited by 16 publications

References 22 publications

Overview of Nongynecological Samples Prepared with Liquid-Based Cytology Medium

Overview of Nongynecological Samples Prepared with Liquid-Based Cytology Medium

Comparison of conventional Papanicolaou cytology samples with liquid-based cervical cytology samples from women in Pernambuco, Brazil

Liquid-based Cytology vs Conventional Cytology as a Screening Tool for Cervical Cancer in Postmenopausal Women

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