Evaluation of the concentration of malondialdehyde and nitrite in patients with sickle cell anemia treated or not with hydroxyurea

Elias, Darcielle Bruna Dias; Freitas, Rivelilson Mendes de; Gonçalves, Raquel; Magalhães, Hemerson Yuri Ferreira; Sousa, Jacqueline Holanda de; Magalhães, Sílvia Maria Meira

doi:10.1590/s1679-45082010ao1731

Cited by 9 publications

(12 citation statements)

References 20 publications

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“…It was also argued that the observed discrepancy regarding antioxidant effects of HU therapy might be due to the short treatment period. 25 In the current study, the mean duration of patients on HU therapy was 3.1 years as opposed to 6 months 25 ; thus, the effect is unlikely to be time dependent. Our data indicated that in β-thalassemia subjects, HU therapy does not offer any protective role against oxidative damage.…”

Section: Discussionmentioning

confidence: 60%

“…10 HU is suggested to produce direct oxidant and indirect antioxidant effects 30 ; however, there have been contradictory reports regarding the protective role of HU therapy in SCD patients. [23][24][25][26] To examine the possible proactive role of HU treatment on oxidative imbalance in β-thalassemia, we studied oxidative stress biomarkers.…”

Section: Discussionmentioning

confidence: 99%

“…Although the underlying mechanisms for the development of oxidative stress in SCD and β-thalassemia are different, 31 the oxidant/antioxidant ratio seems to be more affected in the latter. 14 On the basis of thiobarbituric acid reactive substances (TBARS) and GSH analysis, a few studies reported an antioxidant role of HU in SCD subjects; 23,24 however, investigations on MDA, 25,26 PON1, 26 and vitamin E 26 have suggested that oxidative damage among SCD patients was independent of HU therapy. It was also argued that the observed discrepancy regarding antioxidant effects of HU therapy might be due to the short treatment period.…”

Section: Discussionmentioning

confidence: 99%

“…23,24 However, there have been contradicting reports regarding the protective role of HU therapy on oxidative imbalance among SCD patients. 25,26 This potential effect of HU on oxidative stress in β-thalassemia subjects has not been investigated to date. The current study, therefore, was designed to explore a possible protective role of HU, if any, on oxidative imbalance by estimating serum concentrations of ROS, PON1 paraoxonase, and arylesterase activities and MDA concentrations in β-thalassemia patients receiving HU therapy.…”

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confidence: 99%

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Serum Paraoxonase Activity and Malondialdehyde Serum Concentrations Remain Unaffected in Response to Hydroxyurea Therapy inβ-Thalassemia Patients

Zohaib

Ansari

Hashim

et al. 2015

The Journal of Clinical Pharmacology

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β-Thalassemia is the most common hereditary disorder characterized by reduced production of β-globin chains of hemoglobin A (HbA). In recent years, hydroxyurea (HU) has shown promising therapeutic benefits in patients with β-thalassemia by fetal hemoglobin augmentation. We have analyzed effects of hydroxyurea treatment on oxidative stress in β-thalassemia patients by assessing activities of paraoxonase (PON) and arylesterase along with malondialdehyde (MDA) and total reactive oxygen species (ROS) concentrations. Blood samples from 159 individuals including 56 HU-treated and 58 untreated β-thalassemia patients and 45 healthy controls were analyzed. PON activity was found to be highest in healthy individuals (177.76 ± 4.44 U/mL) as compared to treated (52.67 ± 3.65 U/mL) and untreated (55.11 ± 3.26 U/mL) patients. A similar trend was observed in the case of arylesterase activity in normal, β-thalassemia-treated, and untreated (210.0 ± 11.25 U/mL, 163.03 ± 9.04 U/mL, 139.77 ± 10.10 U/mL) subjects. Serum MDA concentrations (2.59 ± 0.09 nmol/mL, 2.45 ± 0.08 nmol/mL, and 1.15 ± 0.05 nmol/mL) and total ROS concentrations (3.73 ± 0.20 nmol/mL, 3.54 ± 0.23 nmol/mL, and 2.45 ± 0.14 nmol/mL) were significantly elevated in both groups (untreated and treated) as compared to healthy individuals (P < .01). Oxidative stress was found to be markedly elevated in β-thalassemia patients as compared to healthy controls. Insignificant differences were, however, observed in mean concentrations of PON1 paraoxonase and arylesterase activities, serum MDA concentration and total ROS concentrations between HU-treated and untreated patients. We propose that HU therapy alone seems to be ineffective in managing oxidative stress and is likely to offer a better clinical outcome when supplemented with efficient iron chelation therapy and antioxidants.

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Section: Discussionmentioning

confidence: 60%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Serum Paraoxonase Activity and Malondialdehyde Serum Concentrations Remain Unaffected in Response to Hydroxyurea Therapy inβ-Thalassemia Patients

Zohaib

Ansari

Hashim

et al. 2015

The Journal of Clinical Pharmacology

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“…Several studies have demonstrated a significant increase in oxidative stress biomarkers in SCA patients compared with healthy subjects (32-35) . In SCA, oxidative stress occurs by mechanisms intrinsic to the pathophysiology of the disease, such as the increased rate of auto-oxidation associated to Hb S and reduced bioavailability of nitric oxide (NO) (36,37) .…”

Section: Discussionmentioning

confidence: 99%

Impact of iron overload on interleukin-10 levels, biochemical parameters and oxidative stress in patients with sickle cell anemia

Barbosa¹,

Santos²,

Souza³

et al. 2013

Revista Brasileira De Hematologia E Hemoterapia

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ObjectiveThe aim of this study was to evaluate the impact of iron overload on the profile of interleukin-10 levels, biochemical parameters and oxidative stress in sickle cell anemia patients. MethodsA cross-sectional study was performed of 30 patients with molecular diagnosis of sickle cell anemia. Patients were stratified into two groups, according to the presence of iron overload: Iron overload (n = 15) and Non-iron overload (n = 15). Biochemical analyses were performed utilizing the Wiener CM 200 automatic analyzer. The interleukin-10 level was measured by capture ELISA using the BD OptEIAT commercial kit. Oxidative stress parameters were determined by spectrophotometry. Statistical analysis was performed using GraphPad Prism software (version 5.0) and statistical significance was established for p-values < 0.05 in all analyses. ResultsBiochemical analysis revealed significant elevations in the levels of uric acid, triglycerides, very low-density lipoprotein (VLDL), alanine aminotransferase (ALT), lactate dehydrogenase (LDH), urea and creatinine in the Iron overload Group compared to the Non-iron overload Group and significant decreases in the high-density lipoprotein (HDL) and low-density lipoprotein (LDL). Ferritin levels correlated positively with uric acid concentrations (p-value < 0.05). The Iron overload Group showed lower interleukin-10 levels and catalase activity and higher nitrite and malondialdehyde levels compared with the Non-iron overload Group. ConclusionThe results of this study are important to develop further consistent studies that evaluate the effect of iron overload on the inflammatory profile and oxidative stress of patients with sickle cell anemia.

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Monocyte chemoatractant protein-1: A potential biomarker of renal lesion and its relation with oxidative status in sickle cell disease

Santos¹,

Gonçalves²,

Barbosa³

et al. 2015

Blood Cells, Molecules, and Diseases

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Evaluation of the concentration of malondialdehyde and nitrite in patients with sickle cell anemia treated or not with hydroxyurea

Cited by 9 publications

References 20 publications

Serum Paraoxonase Activity and Malondialdehyde Serum Concentrations Remain Unaffected in Response to Hydroxyurea Therapy inβ-Thalassemia Patients

Serum Paraoxonase Activity and Malondialdehyde Serum Concentrations Remain Unaffected in Response to Hydroxyurea Therapy inβ-Thalassemia Patients

Impact of iron overload on interleukin-10 levels, biochemical parameters and oxidative stress in patients with sickle cell anemia

Monocyte chemoatractant protein-1: A potential biomarker of renal lesion and its relation with oxidative status in sickle cell disease

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