The interplay between mutations in cagA, 23S rRNA, gyrA and drug resistance in Helicobacter pylori

Abstract

In this study, we evaluated the mutations of Helicobacter pylori associated with resistance to clarithromycin and levofloxacin. Furthermore, based on the proposed interaction between antimicrobial resistance and pathogenicity, we correlated the mutation profiles of the strains with the presence of the pathogenicity gene cagA. We analyzed 80 gastric biopsy specimens from H. pylori-infected patients for point mutations in the 23S rRNA gene region and in the gyrA gene, which are related to clarithromycin and levofloxacin resistance, respectively, and investigated the presence of the cagA gene in these strains. We observed that in the assayed biopsies, 8.7% (7/80) had mutations in the 23S rRNA gene region at positions 2143 and 2142, while 22.5% (18/80) had mutations in gyrA at codons 87 and 91. Moreover, absence of the CagA-EPIYA pathogenicity factor was observed in 68% (17/25) of resistant samples. The knowledge of the local profile of antimicrobial resistance and the complex interplay involving resistance and pathogenicity can contribute to an appropriate clinical approach.

Clarithromycin-resistant H. pylori primary strains and virulence genotypes in the Northeastern region of Brazil

“…The low prevalence of primary resistance to clarithromycin in H. pylori cagA-positive strains coincides with studies reported from the Southeast region of Brazil 36 . On the contrary, other studies demonstrated that cagA positivity was not associated with resistance status 31,32,37 .…”

Consultório Farmacêutico: Uma Nova Perspectiva Para Promoção De Saúde

“…O mecanismo de resistência aos antibióticos está associado ao uso incorreto dos fármacos, levando a resistência das cepas. Além desses mecanismos desenvolvidos pela H.pylori alguns estudos mostram que a idade e o sexo dos pacientes e a presença ou ausência de patogenicidade das bactérias são fatores importantes no desenvolvimento da resistência bacteriana (VIANNA et al, 2018).…”

New recording genes and genetic mutations of H. pylori genome in gastroduodenal disease patients in Basrah province-Iraq

“…Suzuki et al, (2019) reported that from the 17 studied genes, 5 genes were identified with a mutation coding for outer membrane proteins which is potentially accelerate colonization and inflammation (16). Vianna et al, (2018) identified seven mutation in 23s rRNA region related to antibiotic resistance, whereas the results of Jabar & Alfaisal, (2017) documented no mutations were observed in the codons but the sequencing analysis detect point mutations in exons (17,18). In a study by Dai et al, (2022) which enrolled a total of 133 H. pylori positive patients they reported that no significant link was detected between VacA genotypes patients and antibiotic resistance (19).…”