2017
DOI: 10.1590/s1678-9946201759048
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Abstract: Visceral leishmaniasis, associated with HIV/AIDS coinfection, is becoming a more aggressive disease, complicating an accurate prognosis. A 21-year-old HIV-positive female presenting with clinical features of visceral leishmaniasis was enrolled in this study. Bone marrow cytology, Novy-MacNeal-Nicolle culture and kDNA PCR of peripheral blood were all positive. Typing methods, multilocus enzyme electrophoresis and ITS1-RFLP PCR of peripheral blood confirmed infection by Leishmania (L.) infantum chagasi . PCR has… Show more

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Cited by 6 publications
(7 citation statements)
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“…We obtained PCR with sensitivity of 0.1 pg for specific subgenus reactions, and 1 pg for the reactions that amplify L. amazonensis and L. infantum kDNA sequences. These sensitivities were similar to those obtained in other studies using ITS1 primers [35, 37].…”
Section: Discussionsupporting
confidence: 90%
See 1 more Smart Citation
“…We obtained PCR with sensitivity of 0.1 pg for specific subgenus reactions, and 1 pg for the reactions that amplify L. amazonensis and L. infantum kDNA sequences. These sensitivities were similar to those obtained in other studies using ITS1 primers [35, 37].…”
Section: Discussionsupporting
confidence: 90%
“…The use of PCR for detection of Leishmania DNA in sand flies is a useful technique for the identification of putative vectors in different geographical areas [33, 36-39]. Some targets used to analyze the infection in sand flies are ITS1 [33, 37, 38] and kDNA [36, 40, 41].…”
Section: Discussionmentioning
confidence: 99%
“…infantum kDNA sequences. These sensitivities were similar to those obtained in other studies using ITS1 primers [37, 39]. Species-specific PCR assays with higher sensitivity still need to be developed to detect infections with lower parasite load.…”
Section: Discussionsupporting
confidence: 85%
“…The use of PCR for detection of Leishmania DNA in sand flies is a useful technique for the identification of putative vectors in different geographical areas [35, 3841]. Some targets used to analyze the infection in sand flies are ITS1 [35, 39, 40] and kDNA [38, 42, 43].…”
Section: Discussionmentioning
confidence: 99%
“…Increasing knowledge of the Leishmania genome has allowed the development of high sensitive molecular methods with many advantages over microscopic and serological methods. PCR-based assays have been applied to several biological samples, such as bone marrow, 17 , 18 , 19 spleen, 17 , 19 lymph nodes, 17 blood, 18 , 19 , 20 and urine. 21 , 22 , 23 , 24 , 25 …”
Section: Introductionmentioning
confidence: 99%