Bartonella henselae AS A PUTATIVE CAUSE OF CONGENITAL CHOLESTASIS

Velho, Paulo Eduardo Neves Ferreira; Bellomo-Brandão, Maria Ângela; Drummond, Marina Rovani; Magalhães, Renata Ferreira; Hessel, Gabriel; Barjas‐Castro, Maria de Lourdes; Escanhoela, C.A.F.; Negro, G. Del; Okay, Thelma Suely

doi:10.1590/s1678-9946201658056

Cited by 2 publications

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“…In contrast to those studies, no congenital infections were recognized in bank voles Myodes glareolus in the UK [ 12 ]. Several cases of human congenital bartonellosis have been reported and attributed to different species of Bartonella [ 30 , 31 ], including the case of a 22-day-old boy from Peru [ 32 ]. All of the above findings indicate that further studies on vertical transmission of these vector-borne bacteria are still needed to enable a comprehensive epidemiological assessment of the risk of infection by this route.…”

Section: Introductionmentioning

confidence: 99%

Bartonella infections in three species of Microtus: prevalence and genetic diversity, vertical transmission and the effect of concurrent Babesia microti infection on its success

et al. 2018

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BackgroundBartonella spp. cause persistent bacterial infections in mammals. Although these bacteria are transmitted by blood-feeding arthropods, there is also evidence for vertical transmission in their mammalian hosts. We aimed to determine: (i) the prevalence and diversity of Bartonella spp. in a Microtus spp. community; (ii) whether vertical transmission occurs from infected female voles to their offspring; (iii) the effect of concurrent Babesia microti infection on the success of vertical transmission of Bartonella; and (iv) the impact of congenital infection on pup survival.ResultsWe sampled 124 Microtus arvalis, 76 Microtus oeconomus and 17 Microtus agrestis. In total, 115 embryos were isolated from 21 pregnant females. In the following year 11 pregnant females were kept until they had given birth and weaned their pups (n = 62). Blood smears and PCR targeting the Bartonella-specific rpoB gene fragment (333bp) were used for the detection of Bartonella. Bartonella DNA was detected in 66.8% (145/217) of the wild-caught voles. Bartonella infection was detected in 81.8% (36/44) of pregnant female voles. Bartonella-positive individuals were identified among the embryos (47.1%; 40/85) and in 54.8% (34/62) of pups. Congenitally acquired Bartonella infections and co-infection with B. microti had no impact on the survival of pups over a 3-week period post partum. Among 113 Bartonella sequences, four species were detected: Bartonella taylorii, Bartonella grahamii, Bartonella doshiae and a Bartonella rochalimae-like genotype. Bartonella taylorii clade B was the dominant species in wild-caught voles (49%), pregnant females (47%), their embryos (85%), dams (75%) and pups (95%).ConclusionsHigh prevalence of Bartonella spp. infection maintained in Microtus spp. community is followed by a high rate of vertical transmission of several rodent species of Bartonella in three species of naturally infected voles, M. arvalis, M. oeconomus and M. agrestis. Congenitally acquired Bartonella infection does not affect the survival of pups. Co-infection with B. microti does not affect the effectiveness of the vertical transmission of Bartonella in voles. Bartonella taylorii clade B was found to be the dominant species in wild-caught voles, including pregnant females and dams, and in their offspring, and was also found to be the most successful in vertical transmission.Electronic supplementary materialThe online version of this article (10.1186/s13071-018-3047-6) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

Bartonella infections in three species of Microtus: prevalence and genetic diversity, vertical transmission and the effect of concurrent Babesia microti infection on its success

et al. 2018

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“…Several case reports [ 20 , 34 , 88 – 90 ] and a previous study with blood samples from cats [ 36 ] show that a combination of PCR and different samples increases the chances of detecting the pathogen. The results of this study agree with the literature and reinforce the need to combine several diagnostic tests to avoid false negatives.…”

Section: Discussionmentioning

confidence: 99%

Comparison of molecular methods for Bartonella henselae detection in blood donors

et al. 2023

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The Bartonella genus consists of neglected pathogens associated with potentially transfusional-transmitted and fatal human diseases. We aimed to evaluate Bartonella sp. prevalence in 500 blood donors and compare the results with the data already published about these samples. We used molecular diagnostic methods to detect Bartonella sp.-DNA from blood and liquid culture samples: (A) conventional PCR for two gene regions, the ITS targeting the genus Bartonella and the specific gltA Bartonella henselae; (B) nested PCR for the ftsZ gene and (C) qualitative real-time PCR for the gltA gene, both B. henselae specific. We obtained 30/500 (6%) DNA detections from the blood samples; 77/500 (15.4%) DNA detections from liquid culture samples and five (1%) samples had DNA detection from both. In total, we detected B. henselae DNA from 102/500 (20.4%) donors. The samples used in this study had already been submitted for Bartonella sp.-DNA detection using only a conventional PCR in liquid culture. Sixteen samples (3.2%) were positive previously, and from these 16 samples, 13 were negative in the new investigation. We concluded that the use of liquid culture combined with different molecular tests increases the possibility of detecting Bartonella sp.-DNA, but the tests do not avoid false-negative results. More than a fifth of blood donors had at least one PCR that detected Bartonella sp.-DNA among the eight molecular reactions performed now (four reactions in whole blood and four in liquid culture). Seven percent had B. henselae-DNA detection for two or more distinct regions. Considering the results obtained previously, the DNA of Bartonella spp. was detected or the agent isolated in 23% of analyzed blood donors. The results establish that the low bacteremia and the fastidious characteristics of the bacterium are challenges to laboratory diagnosis and can make it difficult to confirm the infection in patients with bartonelloses.

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Bartonella henselae AS A PUTATIVE CAUSE OF CONGENITAL CHOLESTASIS

Cited by 2 publications

References 17 publications

Bartonella infections in three species of Microtus: prevalence and genetic diversity, vertical transmission and the effect of concurrent Babesia microti infection on its success

Bartonella infections in three species of Microtus: prevalence and genetic diversity, vertical transmission and the effect of concurrent Babesia microti infection on its success

Comparison of molecular methods for Bartonella henselae detection in blood donors

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