Biological characterization of a myotoxin phosphoplipase A2 homologue purified from the venom of the snake Bothrops moojeni

Queiroz,; Mamede, CC; Fonseca, KC; Canabrava, Lcmn; Franca, L C; Silva, Carolina; Stanziola, Leonilda; Beletti, Marcelo Emı́lio; Canabrava, Han; Oliveira, Fábio de

doi:10.1590/s1678-91992011000100007

Cited by 9 publications

(5 citation statements)

References 28 publications

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“…Local tissue damage is a relevant problem caused by Bothrops snake envenomations, resulting from the combined action of several components of venom [ 6 , 9 , 12 , 15 , 41 , 42 ]. BmooMP α -II and B. moojeni crude venom induced myonecrosis when compared with the control group.…”

Section: Resultsmentioning

confidence: 99%

Rapid Purification of a New P-I Class Metalloproteinase fromBothrops moojeniVenom with Antiplatelet Activity

Mamede

Fonseca

et al. 2014

BioMed Research International

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The present study aimed to evaluate the proteolytic and biological activities of a new metalloproteinase from B. moojeni venom. The purification of BmooMPα-II was carried out through two chromatographic steps (ion-exchange and affinity). BmooMPα-II is a monomeric protein with an apparent molecular mass of 22.5 kDa on SDS-PAGE 14% under nonreducing conditions. The N-terminal sequence (FSPRYIELVVVADHGMFTKYKSNLN) revealed homology with other snake venom metalloproteinases, mainly among P-I class. BmooMPα-II cleaves Aα-chain of fibrinogen followed by Bβ-chain, and does not show any effect on the γ-chain. Its optimum temperature and pH for the fibrinogenolytic activity were 30–50°C and pH 8, respectively. The inhibitory effects of EDTA and 1,10-phenantroline on the fibrinogenolytic activity suggest that BmooMPα-II is a metalloproteinase. This proteinase was devoid of haemorrhagic, coagulant, or anticoagulant activities. BmooMPα-II caused morphological alterations in liver, lung, kidney, and muscle of Swiss mice. The enzymatically active protein yet inhibited collagen, ADP, and ristocetin-induced platelet aggregation in a concentration-dependent manner. Our results suggest that BmooMPα-II contributes to the toxic effect of the envenomation and that more investigations to elucidate the mechanisms of inhibition of platelet aggregation may contribute to the studies of snake venom on thrombotic disorders.

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Section: Resultsmentioning

confidence: 99%

Rapid Purification of a New P-I Class Metalloproteinase fromBothrops moojeniVenom with Antiplatelet Activity

Mamede

Fonseca

et al. 2014

BioMed Research International

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“…They are small, secreted proteins of 14-18 kDa that usually contain 6 to 8 disulfide bonds. Queiroz et al (2011) compared their results with other studies (Soares et al 1998;Soares et al, 2000;Borja-Oliveira et al, 2007;Calgarotto, et al, 2008;Santos-Filho et al, 2008), which reported isoforms of PLA 2 myotoxin of B. moojeni with molecular weight ranging between 13,400 Da to 16,500 Da. Thus, we were able to infer from the amino acids residues obtained in our analysis that Bmoo-SII has a molecular weight within this range.…”

Section: Acta Scientiarum Biological Sciencesmentioning

confidence: 84%

<b>Antibacterial activity of different types of snake venom from the Viperidae family against <i>Staphylococcus aureus

Canhas¹,

Heneine²,

Fraga³

et al. 2017

Acta Sci. Biol. Sci.

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ABSTRACT. Toxins and venoms produced by living organisms have exhibited a variety of biological activities against microorganisms. In this study, we tested seven snake venoms from the family Viperidae for antibacterial activity and the activities of reversal of antibiotic resistance and inhibition of biofilm formation against 22 clinical isolates of Staphylococcus aureus. Bothrops moojeni venom exhibited anti staphylococcal activity with the lowest mean value of minimum inhibitory concentration (MIC). Moreover, reversal of antibiotic resistance was observed for combinations of B. moojeni venom (½ x MIC) and norfloxacin or ampicillin (both ½ x MIC) for 86.4% and 50% of the isolates, respectively. B. moojeni venom alone at ½ MIC inhibited 90% of biofilm formation, whereas in combination with ciprofloxacin, both at ½ MIC, a reduction on the NorA efflux pump activity was observed. The detection of in vitro mutants colonies of S. aureus resistant to B. moojeni venom was low and they did not survive. A phospholipase A2 was purified from the venom of B. moojeni and displayed anti-staphylococcal activity when tested alone or in combination with ciprofloxacin. The results presented here will contribute to the search for new antimicrobial agents against resistant S. aureus.

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“…It is found in diverse ecosystems, even in flooded regions, and is responsible for the majority of snakebites in Brazil [1,2]. Bothrops moojeni is a species that has been attracting increased medical attention because its venom is composed of a complex mixture of proteins with biological activity, and it is used as a model to evaluate new drugs [3].…”

Section: Introductionmentioning

confidence: 99%

Cassia occidentalis: effect on healing skin wounds induced by Bothrops moojeni in mice

Delmut¹,

Parente²,

Paula³

et al. 2013

J Pharm Technol Drug Res

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Objective:To evaluate the healing activity of an extract from the leaves of Cassia occidentalis on skin wounds induced by the venom of Bothrops moojeni in mice. Methods:A total of 24 Swiss albino mice were divided into 4 groups: Group 1 animals were treated for 7 days with lanette cream; Group 2 animals were treated for 7 days with 10% leaf extract of Cassia occidentalis in lanette cream; Group 3 animals were treated for 14 days with lanette cream; and Group 4 animals were treated for 14 days with 10% leaf extract of Cassia occidentalis in lanette cream. To induce wounds, the animals were anesthetised intramuscularly after the dorsum cervical region was shaved and inoculated intradermally with 4 μg Bothrops moojeni venom. The gross and histological evaluations were assessed 7 and 14 days after inoculation of the poison. Results:Extract from the leaves of Cassia occidentalis decreased inflammation and epidermal hyperplasia and increased the vascular proliferation and reepithelialisation of wounds induced by Bothrops moojeni venom. Conclusions:These data suggest that the leaves of Cassia occidentalis stimulated the healing of wounds induced by the dermal venom of Bothrops moojeni in mice, and they can be considered an alternative product to treat wounds caused by this snakebites.

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Biological characterization of a myotoxin phosphoplipase A2 homologue purified from the venom of the snake Bothrops moojeni

Cited by 9 publications

References 28 publications

Rapid Purification of a New P-I Class Metalloproteinase fromBothrops moojeniVenom with Antiplatelet Activity

Rapid Purification of a New P-I Class Metalloproteinase fromBothrops moojeniVenom with Antiplatelet Activity

<b>Antibacterial activity of different types of snake venom from the Viperidae family against <i>Staphylococcus aureus

Cassia occidentalis: effect on healing skin wounds induced by Bothrops moojeni in mice

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