Crotalus durissus terrificus venom as a source of antitumoral agents

Soares, Marc A.; Pujatti, PB; Fortes-Dias, CL; Antonelli, Lis Ribeiro do Valle; Santos, RG

doi:10.1590/s1678-91992010000300015

Cited by 10 publications

(9 citation statements)

References 29 publications

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“…Descriptive studies concerning ultrasound monitoring and hormonal evaluation provide information regarding reproduction and the events during estrous cycle and pregnancy, being essential to captive snake handling. The captive reproduction of Crotalus durissus terrificus is an important practice once their venom is a natural source of bioactive substances with therapeutic potential, such as antitumoral properties, as well as for antiophidic serum production (Cura et al, 2002;Soares et al, 2010;Calvete et al, 2011;Kumar et al, 2014;Nudel et al, 2012). The search for new drugs has indicated toxins from Crotalus durissus terrificus venom as inhibitors of cell adhesion, cell migration, epidermal tumor growth factor, metastases induced in experimental mice models, and use of some specific proteins for production of drugs (Cura et al, 2002;Cunha et al, 2015;Neves et al, 2015;Reeks et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Hormonal and ultrasonographic characterization of the seasonal reproductive cycle of male and female Crotalus durissus terrificus

Matayoshi¹,

Souza²,

Gasparotto³

et al. 2018

Anim Reprod

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Research concerning to characterize seasonal reproductive cycle in males and females of Crotalus durissus terrificus by ultrasound and hormonal measurement. Reproductive aspects (follicular and testicular cycles, and pregnancy) from 28 adult snakes (14 males and 14 females) during different months of the years were studied. Snakes housed individually in cages in an environment with controlled luminosity and humidity, and fed monthly. Females were examined by ultrasound during quiescence and active follicular phase, and pregnancy for follicular and embryo/fetal development. Males were evaluated to testicular echotexture and measurements during reproductive and non-reproductive season. The blood samples were collected from males and females for serum testosterone and progesterone determination, respectively. In 77% males the testes were identified by ultrasound and found increased size during summer, decreased serum testosterone in winter, and positive correlation between serum testosterone and testes size. There was no change in testicular echotexture in according to season. Testosterone concentration was decreased during winter and it was positively correlated with testes size. In 71% females, were observed follicular development (vitellogenesis) and gestation since winter to spring by ultrasonography. Parturition occurred mainly in summer. Pregnancy length was 123.0 ± 11.4 days, with mean 6.9 ± 1.5 newborns/female, and there was gradual increase of serum progesterone during this period. There was no variation in progesterone concentration in nongravid females. Males and females Tropical Rattlesnake show seasonal variation of reproductive cycle and was clear a biennial cycle in female. The ultrasonography can be considered an essential tool to accomplish the follicular development, pregnancy and testicular alterations in Tropical Rattlesnake.

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Section: Introductionmentioning

confidence: 99%

Hormonal and ultrasonographic characterization of the seasonal reproductive cycle of male and female Crotalus durissus terrificus

Matayoshi¹,

Souza²,

Gasparotto³

et al. 2018

Anim Reprod

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“…Snake venom is one of the richest sources of bioactive substances in nature and is therefore of great interest for the development of new drugs 4,[14][15][16][17][18][19][20][21][22][23][24][25][26][27][28] . Snake venoms are composed of a mixture of proteins, organic compounds, inorganic ions, carbohydrates, lipid fractions, and other substances 4,14,16,17,20,21,27,29,30 .…”

Section: Crotalus Durissus Terrificus Venom: Composition General Phar...mentioning

confidence: 99%

“…Crotoxin represents approximately 40%-60% of the dry weight of the Cdt venom 4,8,19,33,36,[42][43][44] and is a potent neurotoxin formed by PLA 2 and crotapotin, forming a complex of high toxicity 4,8,32,38,[42][43][44][45][46][47][48][49][50][51][52][53][54] , and exhibits myotoxic, nephrotoxic, and cardiotoxic effects 4,37,38,43,44,46,48,50 . This neurotoxic action is mainly attributed to the inhibitory mechanism of acetylcholine release in presynaptic neurons 48,52,54 .…”

Section: Crotoxinmentioning

confidence: 99%

Antineoplastic properties and pharmacological applications of Crotalus durissus terrificus snake venom

Alves

Júnior

2022

Rev. Soc. Bras. Med. Trop.

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“…sPLA 2 with cytotoxic activity to tumor cells was described in Bothrops neuwiedii [85], Bothrops brazili [86], Naja naja naja [87], among others. Crotoxin, the main polypeptide isolated from C. d. terrificus has shown potent antitumor activity as well the whole venom, highlighting thereby the potential of venom as a source of pharmaceutical templates for cancer therapy [88]. BJcuL, a lectin purified from Bothrops jararacussu venom [89] and a metalloproteinase [90] and a lectin from B. leucurus [91] are other examples of toxins from snake venoms with anticancer potential.…”

Section: Toxins With Anticancer and Cytotoxic Activitiesmentioning

confidence: 99%

Toxins from Venomous Animals: Gene Cloning, Protein Expression and Biotechnological Applications

Fernandes-Pedrosa¹,

Félix-Silva²,

Menezes³

2013

An Integrated View of the Molecular Recognition and Toxinology - From Analytical Procedures to Biomedical Applications

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and binding site on different channels or channel subtypes. The venom is constituted by mucopolysaccharides, hyaluronidases, phospholipases, serotonins, histamines, enzyme inhibitors, antimicrobials and proteins namely neurotoxic peptides. Scorpion peptides presents specificity and high affinity and have been used as pharmacological tools to characterize various receptor proteins involved in normal ion channel functionating, as abnormal channel functionating in cases of diseases. The venoms can be characterized by identification of peptide toxins analysis of the structure of the toxins and also have proven to be among the most and selective antagonists available for voltage-gated channels permeable to K + , Na + , and Ca 2+. The neurotoxic peptides and small proteins lead to dysfunction and provoke pathophysiological actions, such as membrane destabilization, blocking of the central, and peripheral nervous systems or alteration of smooth or skeletal muscle activity [5-8]. Spider venoms are complex mixtures of biologically active compounds of different chemical nature, from salts to peptides and proteins. Specificity of action of some spider toxins is unique along with high toxicity for insects, they can be absolutely harmless for members of other taxons, and this could be essential for investigation of insecticides. Several spider toxins have been identified and characterized biochemically. These include mainly ribonucleotide phosphohydrolase, hyaluronidases, serine proteases, metalloproteases, insecticidal peptides and phospholipases D [9-10]. Venoms from toads and frogs have been extensively isolated and characterized showing molecules endowed with antimicrobial and/or cytotoxic activities [11]. Studies involving the molecular repertoire of the venom of bees and wasps have revealed the partial isolation, characterization and biological activity assays of histamines, dopamines, kinins, phospholipases and hyaluronidases. The venom of caterpillars has been partially characterized and contains mainly ester hydrolases, phospholipases and proteases [12]. The purpose of this chapter is to present the main toxins isolated and characterized from the venom of venomous animals, focusing on their biotechnological and pharmacological applications. 2. Biotechnological and pharmacological applications of snake venom toxins While the initial interest in snake venom research was to understand how to combat effects of snakebites in humans and to elucidate toxins mechanisms, snake venoms have become a fertile area for the discovery of novel products with biotechnological and/or pharmacological applications [13-14]. Since then, many different products have been developed based on purified toxins from snake venoms, as well recent studies have been showing new potential molecules for a variety of applications [15]. 2.1. Toxins acting on cardiovascular system Increase in blood pressure is often a transient physiological response to stressful stimuli, which allows the body to react to dangers or to promptly increase activity. However,...

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Crotalus durissus terrificus venom as a source of antitumoral agents

Cited by 10 publications

References 29 publications

Hormonal and ultrasonographic characterization of the seasonal reproductive cycle of male and female Crotalus durissus terrificus

Hormonal and ultrasonographic characterization of the seasonal reproductive cycle of male and female Crotalus durissus terrificus

Antineoplastic properties and pharmacological applications of Crotalus durissus terrificus snake venom

Toxins from Venomous Animals: Gene Cloning, Protein Expression and Biotechnological Applications

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