In vitro adherence of Candida albicans isolated from patients with chronic periodontitis

Machado, Adriana Gadotti; Komiyama, Edson Yukio; Santos, Silvana Soléo Ferreira dos; Jorge, Antônio Olavo Cardoso; Brighenti, Fernanda Lourenção; Koga-Ito, Cristiane Yumi

doi:10.1590/s1678-77572011005000014

Cited by 20 publications

(20 citation statements)

References 30 publications

(44 reference statements)

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“…The adhesion of yeast cells mediates the colonization and invasion of human tissues; thus, it is considered a crucial virulence factor in yeasts, especially in pathogenic Candida sp. [18]. The quality of hydrophobicity in cells can increase the proclivity of microorganisms for adherence.…”

Section: Adherence Of C Albicans and C Parapsilosis Induced By The mentioning

confidence: 99%

Influence of nicotine on the adherence of Candida albicans ATCC 14053 and Candida parapsilosis ATCC 22019 and expression of selected binding-related genes

Gunasegar

Harun

2017

Biotechnology & Biotechnological Equipment

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Smoking is considered one of the main factors for development of dental plaque. Yeasts have been described to contribute to the plaque community. The aim of this study was to investigate the influence of nicotine on the growth of planktonic cells and biofilm, cell aggregation, surface hydrophobicity, cell adherence and binding-related genes expressed by Candida albicans and Candida parapsilosis in vitro. The relative number of viable fungal cells was determined based on viable plate counts. The biofilm growth was quantified using the crystal violet assay. Reverse transcription-polymerase chain reaction was used to evaluate the regulation of selected genes associated with adherence. The results indicated that nicotine enhanced the growth of both planktonic and biofilm oral fungal cells. Cell surface hydrophobicity and the expression of Hyphal Wall Protein 1 (HWP1) and agglutinin-like sequences 3 (ALS3) of C. albicans and C. parapsilosis were found to increase relative to the nicotine concentrations. The results suggest that nicotine can enhance the growth of C. albicans and C. parapsilosis in vitro and influence their adherence to the surface of microplate wells that mimic as the tooth surface.

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Section: Adherence Of C Albicans and C Parapsilosis Induced By The mentioning

confidence: 99%

Influence of nicotine on the adherence of Candida albicans ATCC 14053 and Candida parapsilosis ATCC 22019 and expression of selected binding-related genes

Gunasegar

Harun

2017

Biotechnology & Biotechnological Equipment

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“…Moreover, this fungus, together with many other opportunistic pathogens, commonly colonizes in human orals. When the host immune defense system is weakened, it may cause various types of oral candidiasis, mainly including pulpitis, thrush and angular cheilitis202122. With the prevalence of risk factors, such as neutropenia, antibiotic treatment, organ transplantation and long-time intensive care unit (ICU) stay, candidiasis-related diseases have been of great concern2324.…”

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confidence: 99%

Inhibition of gold nanoparticles (AuNPs) on pathogenic biofilm formation and invasion to host cells

Lǐ

Zhang

et al. 2016

Sci Rep

134

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Owing to the growing infectious diseases caused by eukaryotic and prokaryotic pathogens, it is urgent to develop novel antimicrobial agents against clinical pathogenic infections. Biofilm formation and invasion into the host cells are vital processes during pathogenic colonization and infection. In this study, we tested the inhibitory effect of Au nanoparticles (AuNPs) on pathogenic growth, biofilm formation and invasion. Interestingly, although the synthesized AuNPs had no significant toxicity to the tested pathogens, Candida albicans and Pseudomonas aeruginosa, the nanoparticles strongly inhibited pathogenic biofilm formation and invasion to dental pulp stem cells (DPSCs). Further investigations revealed that AuNPs abundantly bound to the pathogen cells, which likely contributed to their inhibitory effect on biofilm formation and invasion. Moreover, treatment of AuNPs led to activation of immune response-related genes in DPSCs, which may enhance the activity of host immune system against the pathogens. Zeta potential analysis and polyethylene glycol (PEG)/polyethyleneimine (PEI) coating tests further showed that the interaction between pathogen cells and AuNPs is associated with electrostatic attractions. Our findings shed novel light on the application of nanomaterials in fighting against clinical pathogens, and imply that the traditional growth inhibition test is not the only way to evaluate the drug effect during the screening of antimicrobial agents.

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“…The presence of periodontal pockets and increased gingival crevicular fluid triggers the growth of Candida spp. 35,36 Studies have shown that C. albicans can penetrate periodontal tissues [39][40][41] and adhere to oral cells. In the present study, CFU counts of Candida spp decreased significantly after NSPT in the test group.…”

Section: Discussionmentioning

confidence: 99%

Effects of non‐surgical periodontal therapy on clinical and immunological profile and oral colonization of Candida spp in HIV‐infected patients with chronic periodontitis

Nobre

Pólvora

Silva

et al. 2018

Journal of Periodontology

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In vitro adherence of Candida albicans isolated from patients with chronic periodontitis

Cited by 20 publications

References 30 publications

Influence of nicotine on the adherence of Candida albicans ATCC 14053 and Candida parapsilosis ATCC 22019 and expression of selected binding-related genes

Influence of nicotine on the adherence of Candida albicans ATCC 14053 and Candida parapsilosis ATCC 22019 and expression of selected binding-related genes

Inhibition of gold nanoparticles (AuNPs) on pathogenic biofilm formation and invasion to host cells

Effects of non‐surgical periodontal therapy on clinical and immunological profile and oral colonization of Candida spp in HIV‐infected patients with chronic periodontitis

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