Postmortem acinar autolysis in rat sublingual gland: a morphometric study

Nery, Letícia Rodrigues; Moreira, Carla Ruffeil; Cestari, Tânia Mary; Taga, Rumio; Damante, José Humberto

doi:10.1590/s1678-77572010000500014

Cited by 12 publications

(20 citation statements)

References 18 publications

(60 reference statements)

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“…We agree with Nery, et al 10 (2010) who stated that this phenomenon might be a result of surgical dissection trauma during glandular removal. Regarding the variable "congested blood vessels", it was more frequent in MFERSG patients.…”

Section: Discussionsupporting

confidence: 92%

Morphology and morphometry of the human sublingual glands in mouth floor enlargements of edentulous patients

Tolentino

Azevedo‐Alanis

et al. 2013

J. Appl. Oral Sci.

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Asymptomatic mouth floor enlargements may be observed in edentulous patients. These masses, which protrude from the mouth floor, may complicate the fitting of dentures and require surgery. Whether this "entity" may be considered an anatomical variation of the mouth floor or represent specific alterations in the sublingual gland is not known. ObjectiveThe aim of this work is to investigate the morphological and morphometric aspects of the sublingual glands of edentulous patients with mouth floor enlargements and compare the glands of these patients with the sublingual glands of human cadavers. Material and MethodsMicroscopic evaluation was performed on human sublingual glands from edentulous patients with mouth floor enlargements (n=20) and edentulous cadavers (n=20). The patients and cadavers were of similar ages. The data were compared using Mann-Whitney U, Fisher's exact and Student's t tests (p<0.05). ResultsAcinar atrophy, duct-like structures, mononuclear infiltrates, replacement of parenchyma with fibrous/adipose tissue, mucous extravasation and oncocytosis were similar between the groups (p>0.05). Only the variables "autolysis" and "congested blood vessels" presented statistical difference between groups (p=0.014; p=0.043). The morphometric study revealed that the volume densities of acini, ducts, stroma and adipose tissue were similar between the groups (p>0.05). ConclusionThe microscopic characteristics of the sublingual glands in mouth floor enlargements in edentulous patients correspond to characteristics associated with the normal aging process. The glands are not pathological and represent an age-related alteration that occurs with or without the presence of the mouth floor enlargements.

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Section: Discussionsupporting

confidence: 92%

Morphology and morphometry of the human sublingual glands in mouth floor enlargements of edentulous patients

Tolentino

Azevedo‐Alanis

et al. 2013

J. Appl. Oral Sci.

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“…Nery et al [20] determined a 34.3% increase in the glandular mass of the sublingual gland from 0 to 24 h and reported that this increase is related to transition of liquids originating from the surrounding tissues. In the present study, total volume of the adrenal gland as well as adrenal cortex has been increased, however, a significant decrease in medullary volume (P= 0.03) was detected at 12 h. Marked expansion of cortex was detected.…”

Section: Discussionmentioning

confidence: 99%

Postmortem Changes in Electrolytes and Adrenal Gland at Different Intervals in Adult Male Rats

2016

ARC Journal of Animal and Veterinary Sciences

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The importance of determining time of death is crucial to forensic cases. The ability to use electrolytes, ions and adrenal gland changes to determine the postmortem intervals were examined in this study.A total of 9 rats were used, three rats for determining postmortem "zero hour" the remaining 6 rats were divided equally into two groups to investigate postmortem "six hour" and postmortem "twelve hour".

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“…We consider our samples to be very rich and consistent because the sublingual glands were removed from the cadavers after a short post-mortem period. Moreover, the sublingual glands are rarely addressed in previous studies, where they are typically derived from rats 20 , 24 , 28 . Only the works of the present line of research use human sublingual glands 2 , 14 , 18 , 22 .…”

Section: Discussionmentioning

confidence: 99%

“…Age-related changes in human salivary glands have been described by some studies in humans 2 , 6 - 9 , 18 , 23 , 25 , 29 , 30 and animals 20 , 24 . The literature regarding aging alterations in human sublingual glands is limited by the number of reports, and some phenomena are still unclear.…”

Section: Introductionmentioning

confidence: 97%

Phenotype and cell proliferation activity of duct-like structures in human sublingual glands: a histological and immunohistochemical study

et al. 2015

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There are several age-related microscopic changes in the salivary glands, including the increase in the number of duct-like structures (DLS). However, the true origin and the phenotype of the DLS are not known. Objective To evaluate the phenotype and the cell proliferation index of the DLS of human sublingual glands.Material and Methods Sixty sublingual glands obtained from human cadavers were divided into two groups - 0-30 and 61-90 years old. The phenotype was estimated by immunostaining for cytokeratin 19 (CK 19) and the S-100 protein as well as by the presence of mucin and glycogen. The cell proliferation index was determined by the Ki-67 antibody. The histochemical techniques used periodic acid-Schiff (PAS) and Alcian Blue. In each captured microscopic field, the DLS were counted to establish a percentage for the staining profile. The statistical analysis was accomplished using Student’s t-test, the Mann-Whitney test and Pearson’s correlation coefficient (p<0.05).Results Comparing both groups, only CK 19 showed a statistically significant difference (p=0.033), with the strongest expression in the elderly group. There was no significant difference between PAS and Alcian Blue (p=0.270). In both groups, the immunostaining for CK 19 was stronger than that for S-100 (p=0.004;p<0.001), but there was no correlation between the two immunomarkers (ρ=-0.163; p=0.315). There was no immunostaining for Ki-67.Conclusions DLS demonstrate a ductal phenotypic profile and do not present cell proliferation activity. DLS may represent a regressive process arising from acini or represent the result of metaplasia.

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Postmortem acinar autolysis in rat sublingual gland: a morphometric study

Cited by 12 publications

References 18 publications

Morphology and morphometry of the human sublingual glands in mouth floor enlargements of edentulous patients

Morphology and morphometry of the human sublingual glands in mouth floor enlargements of edentulous patients

Postmortem Changes in Electrolytes and Adrenal Gland at Different Intervals in Adult Male Rats

Phenotype and cell proliferation activity of duct-like structures in human sublingual glands: a histological and immunohistochemical study

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