Evaluation of two morphometric methods of bone loss percentages caused by periodontitis in rats in different locations

Souza, Daniela Martins de; Prado, Fernanda de Almeida; Prado, Marcela de Almeida; Rocha, Rosilene Fernandes da; Carvalho, Yasmin Rodarte

doi:10.1590/s1678-77572010000500011

Cited by 18 publications

(18 citation statements)

References 28 publications

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“…20 Morphometric and histologic procedures were compared for alveolar bone measurement, and no significant differences were found between the two methods when the amount of bone was measured after ligature placement in rats. 21 A recent study 22 morphometrically evaluated bone loss by comparing different locations in experimental periodontitis in rats. The maxilla showed greater bone loss than the mandible, and the bone loss was greater on the buccal side.…”

Section: Discussionmentioning

confidence: 99%

Influence of different durations of estrogen deficiency on alveolar bone loss in rats

et al. 2011

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The purpose of this study was to morphometrically evaluate the influence of different durations of ovariectomy-induced estrogen deficiency on alveolar bone loss associated with ligature-induced bone loss in rats. Sixty female Wistar rats were randomly assigned to ovariectomy (OVX test group) or sham operation (SHAM control group). The OVX and SHAM groups were each distributed into three subgroups of ten rats each according to the duration of estrogen deficiency (30, 60 and 90 postoperative days). In all groups, for the last 30 days of the experimental period, cotton ligatures were placed around the cervix of the right upper second molar; the contralateral tooth was left unligated to serve as a control. The maxillary bones were removed, and the alveolar bone loss was analyzed by measuring the distance between the cementoenamel junction and the alveolar bone crest at the buccal site of the right upper second molar. A comparison between the ligated and unligated groups verified the presence of ligature-induced alveolar bone loss (p < 0.05). No significant differences were observed among the unligated groups (p > 0.05). A significant increase in bone loss was observed when ligation occurred 90 days after ovariectomy compared with the sham group (p < 0.05). These results demonstrate that long-term estrogen deficiency affects ligatureinduced alveolar bone loss.

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Section: Discussionmentioning

confidence: 99%

Influence of different durations of estrogen deficiency on alveolar bone loss in rats

et al. 2011

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“…We used a method described by Souza et al . (34) and Kuhr et al . (35) to quantify the area under the cementum‐enamel junction (CEJ), reflecting periodontal bone loss.…”

Section: Methodsmentioning

confidence: 97%

Removal of SOST or blocking its product sclerostin rescues defects in the periodontitis mouse model

Ren

Han²,

et al. 2015

FASEB j.

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Understanding periodontal ligament (PDL) biology and developing an effective treatment for bone and PDL damage due to periodontitis have been longstanding aims in dental medicine. Here, we first demonstrated by cell lineage tracing and mineral doublelabeling approaches that murine PDL progenitor cells display a 2-and 3-fold higher mineral deposition rate than the periosteum and endosteum at the age of 4 weeks, respectively. We next proved that the pathologic changes in osteocytes (Ocys; changes from a spindle shape to round shape with a >50% reduction in the dendrite number/length, and an increase in SOST) are the key pathologic factors responsible for bone and PDL damage in periostin-null mice (a periodontitis animal model) using a newly developed 3-dimensional FITCImaris technique. Importantly, we proved that deleting the Sost gene (a potent inhibitor of WNT signaling) or blocking sclerostin function by using the mAb in this periodontitis model significantly restores bone and PDL defects (n = 4-5; P < 0.05). Together, identification of the key contribution of the PDL in normal alveolar bone formation, the pathologic changes of the Ocys in periodontitis bone loss, and the novel link between sclerostin and Wnt signaling in the PDL will aid future drug development in the treatment of patients with

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“…Several methods have been applied to assess periodontal bone loss in rats through morphometric [7,8,10,11,17,18], radiographic *Statistically significant (ANOVA and Tukey test; p < 0.05) in columns. [7,19], and histologic [12] or histometric [9] analyses applied to jaws.…”

Section: Discussionmentioning

confidence: 99%

Alveolar bone loss induced by high alcohol consumption in rats

Souza

Silva

Pereira

et al. 2017

BDS

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42 ResumoObjetivo: O objetivo do presente estudo foi avaliar o efeito do consumo regular e constante de álcool em longo prazo na porcentagem do suporte ósseo periodontal remanescente (SOP) e perda óssea periodontal (PO) em ratos adultos. Material e Métodos: Cinquenta e quatro (54) ratos foram divididos em 3 grupos: Controle (ingestão diária de água, n = 18), consumo diário de álcool (20% etanol, n = 18) e consumo social de álcool (20% etanol 2x por semana, n = 18). Os ratos foram tratados com acesso de livre escolha a ambas as frequências de consumo de etanol. Eles foram sacrificados após 90 dias e suas mandíbulas esquerdas foram radiografadas para medição do SOP. As mesmas mandíbulas esquerdas foram dissecadas e coradas. A PO foi avaliada morfometricamente medindo a distância entre a junção cimento-esmalte e a crista óssea alveolar. Resultados: Não houve diferença (p> 0,05) na quantidade de álcool consumido entre os grupos de ingestão diária e social. Os ratos também evidenciaram menor porcentagem de SOP e maior PO (p <0,05) em ambos os grupos de consumo de álcool em comparação com o controle. Conclusão: A mesma quantidade consumida constante e regular de álcool em longo prazo pode causar perda óssea alveolar e reduzir o suporte ósseo periodontal remanescente em ratos adultos. Assim, a perda óssea alveolar foi associada com a quantidade de álcool consumido, ao invés da periodicidade em ratos sem periodontite. ABsTRACTObjectives: The aim of the present study was to assess the effect of regular and constant longterm alcohol consumption on the percentage of the remaining periodontal bone support (PBS) and periodontal bone loss (PBL) in adult rats. Material and Methods: Fifty-four (54) rats were divided into 3 groups: Control (daily water intake, n=18) daily alcohol intake (20% ethanol, n=18) and social alcohol intake (20% ethanol 2x a week, n=18). The rats were treated with free-choice access to both ethanol consumption frequencies. They were euthanized after 90 days and their left mandibles were radiographed for PBS measuring. The same left mandibles were defleshed and stained. The PBL was morphometrically assessed by measuring the distance between cement-enamel junction and alveolar bone crest. Results: Did not show difference (p > 0.05) in the amount of consumed alcohol between the social and daily intake groups. Rats also evidenced lower PBS percentage and higher PBL (p<0.05) in both alcohol consumption groups in comparison to the control. Conclusion: The long-term constant and regular same amount alcohol consumption may cause alveolar bone loss and reduce the remaining periodontal bone support in adult rats. Thus, the alveolar bone loss was associated with the amount of consumed alcohol, rather than with periodicity in periodontitis-free rats.

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Evaluation of two morphometric methods of bone loss percentages caused by periodontitis in rats in different locations

Cited by 18 publications

References 28 publications

Influence of different durations of estrogen deficiency on alveolar bone loss in rats

Influence of different durations of estrogen deficiency on alveolar bone loss in rats

Removal of SOST or blocking its product sclerostin rescues defects in the periodontitis mouse model

Alveolar bone loss induced by high alcohol consumption in rats

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