Immunohistochemical analysis for CD21, CD35, Caldesmon and S100 protein on dendritic cells types in oral lymphomas

Mesquita, Ricardo Alves; Araújo, Vera Cavalcanti de; Paes, Roberto Pinto; Nunes, Fábio Daumas; Souza, Sílvia Ribeiro de

doi:10.1590/s1678-77572009000300023

Cited by 5 publications

(3 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…S100 protein is one of the members of EF-hand type Ca 2+ -binding protein family and is involved in a variety of cellular processes such as cell-cycle regulation and differentiation910. S100 protein is an immunohistochemical marker for interdigitating reticular cells (IRC), a subset of dendritic cells, in lymphoid tissues1112. In order to exclude the possibility of the dendritic cell-associated S100 protein, we have done variety of anti-DC antibody (MHCII, CD11c, CD103 and CD83) expression.Our experimental results show that all FR-labeled nerve endings finally were shown to directly target only S100 cells, which were not express other DC markers (MHCII, CD11c, CD103 and CD83).…”

Section: Discussionmentioning

confidence: 99%

S100+ cells: A new neuro-immune cross-talkers in lymph organs

Huang

Zhu

Zhang

et al. 2013

Sci Rep

View full text Add to dashboard Cite

Up to now, the ‘hardwired’ neural pathway of the neuro-immune regulation is not fully understood. Here we reported a new neural pathway which links sympathetic nerves with immune cells of the lymphoid tissues. Our results demonstrated that nerve fibers derived from superior cervical ganglion directly targeted only S100+ cells in the cervical lymph nodes. Moreover, we found co-expression of neurotransmitters such as norepinephrine, vasoactive intestinal polypeptide and neuropeptide Y in the postganglionic sympathetic nerve endings that innervate S100+ cells. Our findings suggested that S100+ cells serve as a neuro-immune cross-talker in lymph organs that may play a significant role in transmitting signals of nervous cells to targeted immune cells. The new findings provide better understanding of the cross-talk mechanism between the nervous system and the immune system.

show abstract

Section: Discussionmentioning

confidence: 99%

S100+ cells: A new neuro-immune cross-talkers in lymph organs

Huang

Zhu

Zhang

et al. 2013

Sci Rep

View full text Add to dashboard Cite

show abstract

“…h-Caldesmon has gained importance as a smooth-muscle differentiation marker in tumor diagnosis (Miettinen et al 1999;Visée et al 2005), distinguishing myofibroblastic tumors from smooth-muscle tumors (Ceballos et al 2000;Perez-Montiel et al 2006;Qiu et al 2008). Some histopathological studies have also demonstrated the normal distribution of h-caldesmon in additional tissues, including the presence of caldesmon in human FDCs from normal and neoplastic lymph follicles (Tsunoda et al 1999;Mesquita et al 2009), but which cells express caldesmon in spleen and lymph nodes has not been demonstrated to date.…”

Section: Introductionmentioning

confidence: 99%

The Actin-binding Protein Caldesmon Is in Spleen and Lymph Nodes Predominately Expressed by Smooth-muscle Cells, Reticular Cells, and Follicular Dendritic Cells

Köhler

2009

J Histochem Cytochem.

View full text Add to dashboard Cite

Reticular cells and follicular dendritic cells (FDCs) build up a framework that underlies the compartmentalization of spleens and lymph nodes. Subpopulations of reticular cells express the smooth-muscle isoform of actin, indicative of a specialized contractile apparatus. We have investigated the distribution of the actin-binding protein caldesmon in spleen and lymph nodes of mice and rats. Caldesmon modulates contraction and regulates cell motility. Alternative splicing of transcripts from a single gene results in high-molecular-mass isoforms ( h-caldesmon) that are predominately expressed by smooth-muscle cells (SMCs), and low-molecular-mass isoforms ( I-caldesmon) that are thought to be widely distributed in non-muscle tissues, but the distribution of caldesmon in spleen and lymph nodes has not been reported. We have performed Western blot analysis and immunohistochemistry using four different antibodies against caldesmon, among these a newly developed polyclonal antibody directed against recombinant mouse caldesmon. Western blot analysis showed the preponderance of I-caldesmon in spleen and lymph nodes. Our results from immunohistochemistry demonstrate caldesmon in SMCs, as expected, but also in reticular cells and FDCs, and suggest that the isoform highly expressed by reticular cells is I-caldesmon. In spleen of SCID mice, caldesmon was expressed by reticular cells in the absence of lymphocytes.

show abstract

“…As synuclein in the brain is required for facilitating the rapid release of neural transmitters from the axons of neurons, 8 we speculate that g-synuclein in lymph nodes might be used for the analogous function of facilitating the secretion of chemokines, such as CXCL13, from the dendrites of FDCs. 20,34 Ultrastructural studies have shown that FDCs in the primary lymphoid follicles of the lymph node and tonsil are distributed homogeneously. In contrast, FDCs in the GCs of secondary lymphoid follicles are dispersed in a gradient, being densest in the LZ and scarcest in the dark zone (DZ), and conform to the polarized arrangement of centrocytes and centroblasts.…”

Section: Discussionmentioning

confidence: 99%

γ-Synuclein Is a Promising New Marker for Staining Reactive Follicular Dendritic Cells, Follicular Dendritic Cell Sarcoma, Kaposi Sarcoma, and Benign and Malignant Vascular Tumors

Zhang

Maitta

Bhattacharyya

et al. 2011

American Journal of Surgical Pathology

View full text Add to dashboard Cite

Synucleins are small soluble proteins found in normal brain that facilitate rapid release of neurotransmitters. α-synuclein is a major component of the Lewy body of neurodegenerative diseases and γ-synuclein is a marker of aggressive carcinomas. As the role of γ-synuclein has not yet been investigated in the lymphoid system, we immunohistochemically stained normal lymphoid organs, lymph nodes with reactive lymphoid hyperplasia, and malignant lymphomas. The anti-γ-synuclein antibody strongly stained the follicular dendritic cell (FDC) meshworks and vascular and lymphatic endothelial cells in reactive lymphoid tissues, in B-cell lymphomas with a nodular pattern, and in angioimmunoblastic T-cell lymphomas. There were no γ-synuclein-positive FDC meshworks in B-cell or T-cell lymphomas with a diffuse pattern. This is in contrast to CD21, which only stained the arms of the FDCs; γ-synuclein highlighted both the long slender cellular processes and the cell body, thereby clearly demonstrating the number of individual FDCs. In addition, γ-synuclein was strongly expressed by the neoplastic counterpart of reactive FDCs (FDC sarcoma) and by the neoplastic counterparts of normal lymphatic and vascular endothelial cells (Kaposi sarcoma, hemangioma, and angiosarcoma). Only a few spindle cell neoplasms (SSNs) derived from smooth muscle, peripheral nerve, or gastrointestinal stroma expressed γ-synuclein; however, γ-synuclein was not expressed by 11 other types of SSNs tested. These results suggest that γ-synuclein is a promising new adjunct marker for identifying reactive FDCs and for diagnosing FDC sarcoma and benign and malignant vascular tumors.

show abstract

Immunohistochemical analysis for CD21, CD35, Caldesmon and S100 protein on dendritic cells types in oral lymphomas

Cited by 5 publications

References 20 publications

S100+ cells: A new neuro-immune cross-talkers in lymph organs

S100+ cells: A new neuro-immune cross-talkers in lymph organs

The Actin-binding Protein Caldesmon Is in Spleen and Lymph Nodes Predominately Expressed by Smooth-muscle Cells, Reticular Cells, and Follicular Dendritic Cells

γ-Synuclein Is a Promising New Marker for Staining Reactive Follicular Dendritic Cells, Follicular Dendritic Cell Sarcoma, Kaposi Sarcoma, and Benign and Malignant Vascular Tumors

Contact Info

Product

Resources

About