A scanning electron microscopy study of root surface smear layer removal after topical application of EDTA plus a detergent

Abstract

he aim of the present study was to compare root surface smear layer removal following topical application of EDTA and EDTA-T (Texapon). Extracted human teeth had their cementum removed and were mechanically scaled. A total of 220 root specimens were obtained and were randomly assigned to the following groups: I-saline solution (control), II-EDTA; III-EDTA-T. Groups II and III specimens were assigned to different EDTA gel concentrations: 5%, 10%, 15%, 20% and 24%. Smear layer removal score was assessed for each specimen by scanning electron microscopy. The results demonstrated that EDTA and EDTA-T gel led to a higher root surface smear layer removal when compared to the control group. The 5% EDTA gel also showed a higher smear layer removal than the 15%, 20% and 24% EDTA gels (p<0.05). No difference could be found between the different concentrations of EDTA-T gels tested (p>0.05). EDTA gels had statistically significantly lower smear layer scores than the EDTA-T gels for the 5% and 10% concentrations. The results suggested that topical application of EDTA or EDTA-T gel led to significant smear layer removal of the mechanically treated root surfaces. The addition of a detergent to the EDTA gel formula did not improve smear layer removal of the root surface. Uniterms: Periodontal disease; EDTA etching; Smear layer; Root planing. objetivo do presente estudo foi comparar, através de microscopia eletrônica de varredura, a remoção de smear layer de superfícies radiculares após aplicação tópica de gel de EDTA com gel de EDTA-T (Texapon). Foram utilizados dentes humanos que foram submetidos a remoção de cemento radicular e raspagem. As amostras dos dentes foram divididas em 3 grupos: Grupo I-(controle) (n=20); Grupo II-gel de EDTA (pH 7,0) nas concentrações de 5%,10%,15%,20% e 24% (n=100); Grupo IIgel de EDTA-T (pH 7,0) nas concentrações acima descritas (n=100). As fotomicrografias foram avaliadas através de um índice e os dados foram estatisticamente analisados. Ambos os tratamentos com EDTA foram efetivos na remoção de smear layer quando comparados ao grupo controle. O gel de EDTA a 5% demonstrou maior capacidade na remoção de smear layer quando comparado com as concentrações de 15%, 20% e 24% (p<0,05). Não houve diferença entre as concentrações de géis de EDTA-T (p>0,05). Os géis de EDTA foram mais efetivos que o EDTA-T nas concentrações de 5 e 10%. Os resultados sugerem que a aplicação tópica de EDTA ou EDTA-T é efetiva na remoção de smear layer, principamente em baixas concentrações e a adição do Texapon não promove vantagens neste tratamento. Unitermos: Doença periodontal; EDTA, condicionamento; Smear layer; Raspagem. 2005; 13(3): 247-52 J Appl Oral Sci

Guided tissue regeneration of intrabony defects with perforated barrier membranes, simvastatin, and EDTA root surface modification: A clinical and biochemical study

“…In the present study, SMV was used as a flowable gel with methylcellulose as a sustained-release vehicle at a concentration of 1.2% which was reported to be non-toxic, non-allergic, and non-irritating. 30,31 The use of EDTA gel at neutral pH (24%) as a root surface conditioner was carried out for smear layer removal, exposing dentine collagen fibers and increasing the diameter of the dentinal tubules 32,33 which could enhance migration and attachment of fibroblast 34,35 and could allow more SMV local adsorption to the root surface improving its availability. [36][37][38] Many studies reported that EDTA root surface etching could enhance drug availability over the root surfaces or in the GCF like chlorhexidine 39 and doxycycline.…”

Effect of the Association between Citric Acid and EDTA on Root Surface Etching

“…In contrast, EDTA is a chelating agent which combines to calcium molecules from root surfaces removing them from the tooth surface. 21 EDTA inhibited blood element adsorption and adhesion on dentin surface a result that can be attributed to the probably nonremoval of all gel of the root surface. Also, as a calcium chelators at neutral pH 22 EDTA may have inhibited or retarded the coagulation events once calcium ions are essential in the coagulation cascade.…”

Ex vivo analysis of root canal cleaning using Endo-PTC associated to NaOCl and different irrigant solutions

“…These have a creamy or gel-like consistency and they aid in lubricating the root walls thereby diminishing friction through suspension of the detritus resulting from instrument use. 4,10,13,[15][16][17][18][19] Removal of the smear layer impregnated in the dentin walls of the root canals is also aided by the demineralizing and chelating action, respectively, of organic and inorganic acids such as citric acid and EDTA, with or without associated detergents. 2,7,8,20,21 Adding a detergent solution to the original Fór-mulation of EDTA has led to increased surface tension for this substance, with the result that greater permeability of the dentin tubules and better cleaning of the dentin walls of the root canals has thereby been provided.…”