Improved model for the establishment and evaluation of detrusor overactivity in female Wistar rats

Abstract

ARTICLE INFO ______________________________________________________________ ______________________Objective: To improve the model for establishment and evaluation of detrusor overactivity in female Wistar rats. Materials and Methods:We ligated the perineal urethra of female Wistar rats and then performed filling cystometry. The probability of detrusor overactivity, bladder capacity, peak voiding pressure and histological changes were investigated.Results: Detrusor overactivity ratio of the obstruction group was 32.4%. Bladder capacity increased from 0.273 ± 0.036mL in control group to 0.89 ± 0.19mL in detrusor overactivity group (P < 0.001), and peak voiding pressure increased from 45.9 ± 4.1 cm.H 2 O to 63.5 ± 17.4cm.H 2 O (P = 0.007). For obstruction group, compared to no detrusor overactivity rats, detrusor overactivity rats had higher bladder capacity (0.89 ± 0.19mL versus 0.43 ± 0.09mL, P < 0.001) and higher peak voiding pressure (63.5 ± 17.4cm.H 2 O versus 44.8 ± 6.2cm.H 2 O, P = 0.005). Detrusor overactivity rats were classified according to peak voiding pressure (49.2 ± 4.2cm.H 2 O versus 80.8 ± 7.1cm.H 2 O, P < 0.001). Moreover, bladder weight increased significantly in detrusor overactivity rats (P = 0.003, P = 0.028) and detrusor histological hypertrophy was observed. Conclusions: Ligating perineal urethra and filling cystometry with intra-urethral cannula approach is a simple and easily reproducible method to establish and evaluate the model of detrusor overactivity in rats.

Benefits and limitations of animal models in partial bladder outlet obstruction for translational research

“…; Table ). This procedure did not require a low abdominal incision, and impairment of the bladder and its innervation and periurethral blood vessel was avoided for both sexes . This model showed results that were similar to the transperitoneal model with regard to histological changes and cystometry data.…”

Development of an Improved Animal Model of Overactive Bladder: Transperineal LigationversusTransperitoneal Ligation in Male Rats

“…Blood supply and innervation are important for normal bladder function, meaning that such injuries can cause unnecessary and unpredictable variations in OAB models [19]. …”

“…The transperineal model for OAB was introduced by Zhang et al [19] and applied to female rats. We therefore applied the same model to male rats.…”

“…1) [19]. The experimental animals were anesthetized using an intramuscular injection of 40 mg/kg of ketamine and 20 mg/kg of xylazine with the rats in the supine position.…”

“…The abdomen is opened through a midline incision and the tissue around the bladder is dissected, so injuries affecting bladder function can occur. Therefore, Zhang et al [19] introduced an improved model for OAB in female rats using a trans-perineal approach. This method involves making a suture around the perineal urethra to induce partial obstruction in female rats, and the researchers evaluated the effects of this model on bladder function.…”