Development of an Improved Animal Model of Overactive Bladder: Transperineal Ligation<i>versus</i>Transperitoneal Ligation in Male Rats

Kim, Woo Hyun; Bae, Woong Jin; Park, Jung‐Woo; Choi, Jin Bong; Kim, Su Jin; Cho, Hyuk Jin; Ha, U-Syn; Hong, Sung Hoo; Lee, Ji Youl; Hwang, Sung Yeoun; Kim, Sae Woong

doi:10.5534/wjmh.2016.34.2.137

Cited by 10 publications

(10 citation statements)

References 25 publications

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“…This model showed results that were similar to the transperitoneal model with regard to histological changes and cystometry data. However, Kim reported that OAB parameters changed less in the transperineal model than in the transperitoneal model, so the transperineal approach could be less effective in simulating OAB . Furthermore, erectile function could be changed, because cavernous injury is inevitable during the transperineal approach procedure.…”

Section: Techniques Of Pboo Proceduresmentioning

confidence: 99%

Benefits and limitations of animal models in partial bladder outlet obstruction for translational research

et al. 2017

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Abbreviations & Acronyms AMPA = a-amino-3-hydroxy-5-methylisoxazole-4-propionic acid BOO = bladder outlet obstruction BPH = benign prostatic hyperplasia DO = detrusor overactivity HIF-1 = hypoxia-inducible factor-1 IL = interleukin LUTS = lower urinary tract symptoms NGF = nerve growth factor NO = nitric oxide NVC = non-voiding contraction OAB = overactive bladder pBOO = partial bladder outlet obstruction TGF-b = transforming growth factor-b Abstract: The functions of the lower urinary tract have been investigated for more than a century. Lower urinary tract symptoms, such as incomplete bladder emptying, weak urine stream, daytime urinary frequency, urgency, urge incontinence and nocturia after partial bladder outlet obstruction, is a frequent cause of benign prostatic hyperplasia in aging men. However, the pathophysiological mechanisms have not been fully elucidated. The use of animal models is absolutely imperative for understanding the pathophysiological processes involved in bladder dysfunction. Surgical induction has been used to study lower urinary tract functions of numerous animal species, such as pig, dog, rabbit, guinea pig, rat and mouse, of both sexes. Several morphological and functional modifications under partial bladder outlet obstruction have not only been observed in the bladder, but also in the central nervous system. Understanding the changes of the lower urinary tract functions induced by partial bladder outlet obstruction would also contribute to appropriate drug development for treating these pathophysiological conditions. In the present review, we discuss techniques for creating partial bladder outlet obstruction, the characteristics of several species, as well as issues of each model, and their translational value.

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Section: Techniques Of Pboo Proceduresmentioning

confidence: 99%

Benefits and limitations of animal models in partial bladder outlet obstruction for translational research

et al. 2017

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“…Animal models used in overactive bladder (OAB) research include partial BOO, spontaneous hypertensive rat, bladder stimulation models using various drugs, nerve damage models, and some gene knock-outs; nonetheless, these models all have individual strengths and weaknesses [ 7 ]. Considering the difficulty of defining DO objectively, the BOO model is one of the most reproducible DO models [ 8 ]. However, when in vivo urodynamic tests are conducted to observe the relationship between pressure and flow from the bladder, it is impossible to observe the functional changes of the bladder alone, because some residual urine is usually present in the BOO model due to the mechanically fixed obstruction of the urethra.…”

Section: Introductionmentioning

confidence: 99%

Near-Normalized Gene Expression Profiles in Bladder With Detrusor Overactivity in Rats With Bladder Outlet Obstruction After Deobstruction

et al. 2017

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PurposeThe pathophysiological role of detrusor overactivity (DO) in the bladder, which is commonly observed in various bladder diseases, is not well understood. DO appears in bladder outlet obstruction (BOO), and may continue even after subsequent deobstruction. DO therefore provides an excellent opportunity to observe molecular biological changes.MethodsIn this study, to understand the molecular effects of persistent DO after BOO induction and deobstruction, we performed awake cystometry on female Sprague-Dawley rats divided into 4 groups: a sham group, a BOO group, a deobstructed group with DO after BOO (DDO), and a deobstructed group without DO after BOO (non-DDO). Total RNA was extracted from the bladder samples, and gene expression profiles were compared between the sham and model groups.ResultsDO was observed in 5 of the 6 rats (83%) in the BOO group, and in 6 of the 13 rats (46%) in the deobstructed group. The non-DDO group showed a significantly greater residual volume than the DDO group. Through a clustering analysis of gene expression profiles, we identified 7,532 common upregulated and downregulated genes, the expression of which changed by more than 2 fold. In the BOO group, 898 upregulated and 2,911 downregulated genes were identified. The non-DDO group showed 3,472 upregulated and 4,025 downregulated genes, whereas in the DDO group, only 145 and 72 genes were upregulated and downregulated, respectively.ConclusionsAbnormal function and gene expression profiles in bladders after BOO were normalized in the BOO rats with DO after deobstruction, whereas in those without DO, abnormal function persisted and the gene expression profile became more abnormal. DO may play a protective role against the stress to the bladder induced by BOO and deobstruction as a form of adaptive neuroplasticity.

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“…This method has been widely used for the analysis of detrusor overactivity (DO) in rat models. In this study, partial BOO was surgically induced through a transperitoneal approach, as previously described [7,8]. A 25-gauge polyethylene tube was placed on top of the urethral-vesical junction and ligated using 3-0 silk with similar forces in all rats.…”

Section: Transperitoneal Ligation Of the Urethramentioning

confidence: 99%

The effects of oral administration of the novel muscarinic receptor antagonist DA-8010 on overactive bladder in rat with bladder outlet obstruction

et al. 2020

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Background: DA-8010 is a novel compound developed for the treatment of overactive bladder (OAB) and urinary incontinence. The aims of this study were to investigate the effects of DA-8010 on OAB in a rat model. Methods: Study animals were divided into the following five groups of seven animals each: a sham-operated control group, a control group with partial bladder outlet obstruction (BOO) (OAB group), and three DA-8010 (doses of 0.3 mg/kg/day, 1 mg/kg/day, and 3 mg/kg/day, respectively) with partial BOO groups. Oral administration of the drugs was continued for 14 days after 2 weeks of partial BOO. After 4 weeks of partial BOO, cystometrography was performed in all groups. Additionally, pro-inflammatory cytokines, Rho-kinases, and histology of the bladder were analyzed. Results: There was a significant increase in the contraction interval and a decrease in contraction pressure in the 3 mg/kg/day DA-8010 group versus those in the OAB group. Rho kinase was also significantly decreased in the DA-8010 3 mg/kg/day dosage treatment group. The increased ratio of collagen to smooth muscle after partial BOO was significantly attenuated in the DA-8010 3 mg/kg/day dosage group. Conclusions: Oral administration of DA-8010 at 3 mg/kg/day improved findings in an OAB rat model induced by partial BOO. Our results suggest that the novel muscarinic receptor antagonist DA-8010 may be a promising drug for treating patients with OAB.

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Development of an Improved Animal Model of Overactive Bladder: Transperineal LigationversusTransperitoneal Ligation in Male Rats

Cited by 10 publications

References 25 publications

Benefits and limitations of animal models in partial bladder outlet obstruction for translational research

Benefits and limitations of animal models in partial bladder outlet obstruction for translational research

Near-Normalized Gene Expression Profiles in Bladder With Detrusor Overactivity in Rats With Bladder Outlet Obstruction After Deobstruction

The effects of oral administration of the novel muscarinic receptor antagonist DA-8010 on overactive bladder in rat with bladder outlet obstruction

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