Beneficial effect of pentoxifylline into the testis of rats in an experimental model of unilateral hindlimb ischemia/reperfusion injury

Takhtfooladi, Mohammad Ashrafzadeh; Moayer, Fariborz; Takhtfooladi, Hamed Ashrafzadeh

doi:10.1590/s1677-5538.ibju.2014.0263

Cited by 16 publications

(11 citation statements)

References 39 publications

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“…SOD and glutathione peroxidase are major enzymes that scavenge harmful ROS in male reproductive organs 24 . Previous studies have shown similar findings that I/R leads to increased MDA and inactivation of antioxidant enzymes in rat testes 23,25,26 . ROS Metformin is a biguanide drug which improves the sensitivity to insulin, increases the insulin-stimulated uptake and utilization of glucose, reduces basal hepatic glucose production, causes weight reduction and decreases hunger 28 .…”

Section: Discussionsupporting

confidence: 74%

Protective effect of metformin on testicular ischemia/reperfusion injury in rats

et al. 2016

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PURPOSE:To investigate the protective effect of metformin on testicular ischemia/reperfusion (I/R) injury in rats. METHODS:Eighteen adult male Wistar rats were randomly divided into three experimental groups (n=6), as follows: Sham, I/R, and Metformin. 1-hour ischemia was induced by the left testicular artery and vein clipping followed by 7 days of reperfusion. Metformin (100 mg/kg) was administrated orally for 7 days via oral gavage after ischemic period. At the end of trial, the left testis was removed for histological analysis and oxidative stress measurement.RESULTS: I/R reduced superoxide dismutase (SOD) activities and testicular Johnsen's scores accompanied by an elevation in malondialdehyde (MDA) and myeloperoxidase (MPO) levels in comparison with the sham group (P < 0.05). Compared to I/R group, metformin restored testicular Johnsen's scores, SOD activity, MDA and MPO levels (P < 0.05). CONCLUSION:Metformin has a protective effect against I/R injury on the testis.

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Section: Discussionsupporting

confidence: 74%

Protective effect of metformin on testicular ischemia/reperfusion injury in rats

et al. 2016

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“…For example, a study conducted by Takhtfooladi et al. showed that administration of 40 mg/kg pentoxifylline significantly reduced testicular injury in a rat model of unilateral hindlimb ischemia/reperfusion injury [ 44 ]. In the PTX treated group, histopathological features such as edema, congestion, hemorrhage, and necrosis of the germinal cells were markedly less when compared to ischemia-reperfusion group.…”

Section: Discussionmentioning

confidence: 99%

Pentoxifylline improves the survival of spermatogenic cells via oxidative stress suppression and upregulation of PI3K/AKT pathway in mouse model of testicular torsion-detorsion

Dhulqarnain

Takzaree

Hassanzadeh

et al. 2021

Heliyon

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Testicular torsion-detorsion results in enhanced formation of free radicals which contribute to the pathophysiology of testicular tissue damage. Recent reports have identified protective role of pentoxifylline (PTX) against free radicals. Thus, we determined the protective effect of pentoxifylline against testicular damage in mouse model of testicular torsion-detorsion. Twenty (6 weeks old) male mice were divided into 4 groups of 5 animals each namely: Control (sham operated group), T1 (Torsion-detosion + single dose 100 mg/kg PTX, T2 (torsion-detorsion + 20 mg/kg PTX for 2 weeks and T/D (torsion-detorsion only). Animals in T1, T2 and T/D groups underwent 2 h of testicular torsion with the left testes rotated 720° (clockwisely) followed by 30 min of detorsion. After detorsion, drug administration was done intraperitoneally. The left testes of all the animals were excised on the 35th day after torsion-detortion for histopathological and biochemical assay. Histomorphological analysis of the seminiferous tubules showed that there were significant increase (P < 0.01 or 0.05) in the mean seminiferous tubule diameter, Johnson score and germ cells of animals in Control and T1 compared to T2 and T/D with no significant difference (P > 0.05) in testes weight, sertoli, leydig and myoid cells in all groups. IHC results showed significant increase (P < 0.01 or 0.05) in id4 and scp3 protein markers in Control, T1 and T2 compared to T/D. Oxidative stress analysis revealed that Pentoxifylline significantly increased (P < 0.01 or 0.05) the level of SOD, catalase, mRNA expression of akt and pi3k genes but significantly suppress (P < 0.01 or 0.05) MDA and Caspase-3 level in Control, T1 and T2 compared to T/D. Pentoxifylline could be used as an adjunct therapy to surgery in the treatment of torsion-detorsion related testicular injury, However, Further studies are needed to evaluate the effects of pentoxifylline on testicular torsion.

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“…Additionally, SOD and GPx are major enzymes that scavenge harmful ROS in male reproductive system 26 . Based on the reports, I/R increased MDA and decrease SOD as well as GPx which leads to formation of inflammatory mediators 15 .…”

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confidence: 99%

“…The FA (2, 5 and 10 mg/kg) significantly increased tissue SOD and GPx activity in I/R rat ( Table 1). Supplementation of the FA decreases the risk of heart and limb defects 26 and urinary tract anomalies [29][30] . The I/R injury in testis resulted in decrease in spermatogenesis 31 .…”

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confidence: 99%

Effects of folic acid administration on testicular ischemia/reperfusion injury in rats

et al. 2017

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1 8-Experimental SurgeryActa Cir Bras. 2017;32(9):755-766 Abstract Purpose: To determine the effect of folic acid (FA) on experimental testicular ischemia/reperfusion (I/R) in rats. Methods: Sixty male Wistar rats were randomly divided into 6 groups. The control group received physiologic saline orally. The sham-operated group received physiologic saline orally then exposed to midline laparotomy without clamping the IR. The I/R rats received oral gavage of the saline then subjected to 1h ischemia /24h reperfusion, period. In folic acid (2mg/ kg+IR) rats received oral gavage of the FA (2mg/kg) then subjected to 1h I/24h R. groups 5-6 received FA (5 and 10 mg/kg), then subjected to 1 h I/24 h, respectively. At the end of the study, semen samples were collected for spermatozoa characteristics. The left testis was removed for histological analysis and superoxide dismutase (SOD), malondialdehyde (MDA) and glutathione peroxidase (GPx) measurement. Results: Spermatozoa mobility, mortality (%) significantly decreased in I/R group (P<0.05). Dose dependent increase observed on spermatozoa mobility, mortality (%) using different levels of the FA (2, 5 and 10 mg/kg) treated rat (P<0.05). Tissue MDA levels significantly increased in I/R rat (P<0.05) while FA (2, 5 and 10mg/kg) in a dose dependent manner decreased I/R-induced MDA (P<0.05). Experimental I/R significantly decreased SOD and GPx activity (P<0.05). Administration of the FA (2, 5 and 10mg/kg) significantly increased tissue SOD and GPx activity in I/R rat (P<0.05). Seminiferous tubules degenerated and loss of spermatogenesis with few spermatocytes was observed in degenerated testis tubules in I/R rat. Orally administration of the FA (5 and 10 mg/kg) improved testis characteristics with few normal seminiferous tubules and spermatocyte in seminiferous tubules in experimental I/Rinduced rat. Conclusion:The treatment of folic acid had a benefit effect against ischemia-reperfusion.

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Beneficial effect of pentoxifylline into the testis of rats in an experimental model of unilateral hindlimb ischemia/reperfusion injury

Cited by 16 publications

References 39 publications

Protective effect of metformin on testicular ischemia/reperfusion injury in rats

Protective effect of metformin on testicular ischemia/reperfusion injury in rats

Pentoxifylline improves the survival of spermatogenic cells via oxidative stress suppression and upregulation of PI3K/AKT pathway in mouse model of testicular torsion-detorsion

Effects of folic acid administration on testicular ischemia/reperfusion injury in rats

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