Effects of low-level laser therapy on the progress of wound healing in humans: the contribution of in vitro and in vivo experimental studies

Abstract

Efeitos da terapia a laser de baixa intensidade na evolução da cicatrização de lesões em humanos: a contribuição dos estudos experimentais in vitro e in vivo

Efeitos bioestimulantes do laser de baixa potência no processo de reparo

“…Moreover, laser radiation increases the synthesis of ATP within mitochondria, thus accelerating the speed of cell mitosis. 11,[19][20][21] In accordance with many authors cited in this study, 4,12,17,21,22 intracellular metabolic changes resulting from biostimulation promoted by lowpower laser have resulted in: accelerated cell division, especially of fibroblasts and epithelial and endothelial cells; rapid production of extracellular matrix, particularly of collagen fibers; movement of leukocytes, fibroblasts and epithelial cells; and increased phagocytic activity of macrophages.…”

“…4,12 Still in accordance with the authors, when laser light interacts with cells and tissues in an appropriate dosage, certain cell functions can be stimulated, among them are stimulation of lymphocytes, mast cell activation, increase of mitochondrial ATP production and proliferation of several types of cells, thus promoting anti-inflammatory effects.…”

“…[4][5][6]12,21,22 Many investigations have attempted to determine the biological effects (analgesic, antiinflammatory and healing) of low-intensity lasers on tissues, especially during the repair process. However, not all of them have produced satisfactory results.…”

Development and Evaluation of Fiber Optic Probe‐based Helium–Neon Low‐level Laser Therapy System for Tissue Regeneration—An In Vivo Experimental Study

“…The wound contraction, which is the primary response of tissue regeneration occurring throughout the healing progression (41), was considered as one of the monitoring parameters of wound healing. As the stimulation of cell functions responsible for wound healing by LLLT depends on the applied optimal dose (42), the rate of wound contraction was observed to increase linearly for the optimal laser dose‐treated animals compared with the unilluminated controls. Although maximum contraction was observed in the reference standard treated group in all the postwounding days as anticipated, these values were not found to be significant compared with the outcome of the optimal laser dose of 2 J cm −2 in our study.…”

Inibição da expressão de ciclooxigenase 2 em feridas cutâneas de camundongos NOD submetidos à terapia a laser de baixa intensidade

“…Fisiologicamente, o processo de cicatrização de feridas passa por uma sequência de eventos que envolvem: hemostasia, inflamação (limpeza), proliferação, amadurecimento ou remodelamento e desconstrução ou digestão [23][24][25] . Quando ocorre uma lesão tecidual, o processo de reparo é modulado pela atividade celular das células da resposta inflamatória e das células da borda da lesão e por uma rede de citocinas e de fatores de crescimento que influenciam a migração, a proliferação e a diferenciação celular para o local da lesão tecidual 26,27 .…”