Effects of in vitro triacontanol on growth, antioxidant enzymes, and photosynthetic characteristics in Arachis hypogaea L.

Abstract

ABstrACt:In vitro effects of triacontanol (TRIA) on antioxidant enzymes and photosynthetic characteristics were studied in Arachis hypogaea L. cultivars (M-13 and PBS24030). The in vitro impact of TRIA on multiplication potential was also evaluated, which was found to be best at 2.0 ml L -1 TRIA in combination with 6-Benzyl adenine (BA, 3 mg L -1 ) in both the groundnut cultivars. Rhizogenesis was observed in almost all the TRIA treated cultures in both the cultivars. Moreover, shoots failed to set roots, in the presence of BA. Total chlorophyll content, total soluble sugars, total soluble proteins and ascorbate peroxidase (APX; EC 1.11.1.11) activity were found to be increasing with increasing concentrations of TRIA, whether alone or in combination with BA, however, genotypic differences were observed in case of Hill reaction activity (HRA) in between the cultivars. Best catalase (CAT; EC 1.11.1.6) activity was reported at 1.0 ml L -1 TRIA with BA (3 mg L -1 ) in both the cultivars. Peroxidase (POX; EC 1.11.1.7) and polyphenol oxidase (PPX; 1.14.18.1) activity was maximum at 1.0 ml L -1 TRIA with BA (3 mg L -1 ) in M-13, whereas at 2.0 ml L -1 TRIA with BA (3 mg L -1 ) in PBS24030. Lipid peroxidation was found to be reduced with the supplementation of TRIA. The results obtained in the study clearly indicated not only the in vitro establishment of groundnut cultivars in the presence of TRIA but also its effect on various growth promontory physiological parameters.

Triacontanol-induced Regulation in the Key Osmoprotectants and Oxidative Defense System of Sunflower Plants at Various Growth Stages under Salt Stress

“…In contrast to our findings, the increased total soluble protein has been reported under saline regimes in wheat (Zheng et al, 2009;Farouk, 2011;Barakat et al, 2013;Perveen et al, 2013), Erythrina variegata seedlings (Muthuchelian et al, 2003), green gram (Kumaravelu et al, 2000) and Arachis hypogaea L. (Verma et al, 2011). Similarly, Krishnan and Kumari (2008) also reported TRIA treated soybean plants with improved protein content under saline conditions.…”

Study of interaction effect between triacontanol and nitric oxide on alleviating of oxidative stress arsenic toxicity in coriander seedlings

“…NO supplied exogenously provides resistance against stress induced by HMs like Cd in rice (Panda et al 2011) and As in fescue (Jin et al 2010). There is some evidence that the exogenous application of TRIA inhibited lipid peroxidation in spinach and peanut plants' (Ramanarayan et al 2000;Verma et al 2011) leaves and improved membrane integrity by differentially modulating membrane lipid composition (Swamy et al 2009). Perveen et al (2011) found that TRIA-induced improvement in growth might have been due to its effect on the performance of antioxidant enzymes like POD under salt stress (Perveen et al 2011).…”

Triacontanol-induced changes in growth, yield, leaf water relations, oxidative defense system, minerals, and some key osmoprotectants in Triticum aestivum under saline conditions

“…S-24; however, foliar-applied TRIA at different growth stages decreased oxidative stress-induced membrane damage, which is apparent from the stressinduced reduction in MDA content (a product of lipid peroxidation) and H 2 O 2 (most stable ROS in plants) in both wheat cultivars. TRIA inhibited lipid peroxidation in spinach (Spinacea oleracea L.) (Ramanarayan et al, 2000) and Arachis hypogaea L. (Verma et al, 2011) leaves and improved membrane integrity by differentially modulating membrane lipid composition (Swamy et al, 2009). In the present study, TRIA-induced improvement in growth might have been due to its effect on the performance of antioxidant enzymes like POD under salt stress Ertani et al, 2012).…”