Pretreatment with H2O2 in maize seeds: effects on germination and seedling acclimation to salt stress

Gondim, Franklin Aragão; Gomes-Filho, Enéas; Lacerda, Claudivan Feitosa de; Prisco, José Tarquı́nio; Neto, André D. Azevedo; Marques, Elton Camelo

doi:10.1590/s1677-04202010000200004

Cited by 54 publications

(37 citation statements)

References 32 publications

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“…A MFPA foi afetada positivamente pela aplicação do H 2 O 2 até a concentração de 7 µmol L -1 , que promoveu valor máximo de 31,77 g por planta (Figura 5A). Tal fenômeno pode estar relacionado ao efeito desta dose sobre a aclimatação e o sistema de defesa das plantas ao estresse salino, incluindo ativação de sistema oxidativo enzimático, acúmulo de solutos orgânicos, redução na peroxidação de lipídeos e dano de membrana (AZEVEDO NETO et al, 2005;GONDIM et al, 2010;GONDIM et al, 2011;CARVALHO et al, 2011).…”

Section: Resultsunclassified

“…Azevedo Neto et al (2005) constataram que o pré-tratamento de plântulas de arroz utilizando H 2 O 2 em solução nutritiva, induziu a aclimatação, conferindo maior tolerância à salinidade. Gondim et al (2010), verificaram efeito semelhante em plantas de milho utilizando o pré-tratamento das sementes com H 2 O 2 . Outrossim, Gondim et al (2011) observaram que a pulverização de plantas de milho com H 2 O 2 induziu aclimatação das plantas ao estresse salino, revertendo parcialmente os efeitos deletérios da salinidade no crescimento.…”

Section: Introductionunclassified

See 1 more Smart Citation

Métodos de aplicação de diferentes concentrações de H2O2 em milho sob estresse salino

Silva¹,

Lacerda²,

Medeiros³

et al. 2016

Revista Verde

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Resumo: A aplicação exógena de H 2 O 2 tem demonstrado eficiência na aclimatação das plantas aos estresses bióticos e abióticos devido estimular a ativação do sistema de defesa antioxidativo. Neste sentido, objetivou-se com o trabalho avaliar a influência do método de aplicação de diferentes concentrações de H 2 O 2 sobre o crescimento inicial de plantas de milho irrigadas água salina. O experimento foi conduzido em ambiente protegido, com os tratamentos dispostos em delineamento inteiramente casualizado, em esquema fatorial 5 x 2, correspondente as concentrações de peróxido de hidrogênio (H 2 O 2 ) de 0; 5; 10; 15 e 20 µmol L -1 aplicados na semeadura via água de irrigação e, pulverização foliar aos 15 dias após a semeadura (DAS), com quatro repetições e unidade experimental constituída por um vaso de 3 L contendo duas plantas de milho híbrido 4051. As plantas foram irrigadas com água de CE de 2,0 dS m -1 . As 28 DAS verificou-se que a aplicação de H 2 O 2 na semeadura e através de pulverização foliar promove aclimatação de plantas de milho à salinidade da água de irrigação, aumentando a tolerância ao estresse salino. O maior crescimento inicial do milho é obtido nas concentrações de H 2 O 2 variando de 7 a 8 µmol L -1 , sendo mais eficiente a aplicação na semeadura. O pré-tratamento de plantas de milho com H 2 O 2 a partir de 15 µmol L -1 incrementa o estresse, promovendo maiores danos ao crescimento vegetativo. Palavras-chaves:Zea mays L., estresse salino, peróxido de hidrogênio, aclimatação. Abstract:Exogenous application of H 2 O 2 has established efficiency in acclimatization of plants to biotic and abiotic stresses due to stimulate the activation of antioxidant defense system. In this context, it was aimed with this work evaluate the effect of application method of different concentrations of H 2 O 2 on initial growth of maize plants irrigated saline water. The experiment was conducted in a greenhouse, with the treatments in a randomized completely design in a factorial 5 x 2 corresponding the hydrogen peroxide concentrations (H 2 O 2 ) 0; 5; 10; 15:20 µmol L -1 applied in sowing by irrigation water and foliar spray at 15 days after sowing (DAS), with four repetitions and experimental unit consisting of vessel a 3 L containing two plants hybrid maize 4051. The plants were irrigated with EC water of 2.0 dS m -1 . The 28 DAS it was found what the application H 2 O 2 at sowing and foliar spraying promote acclimatization of maize plants at salinity irrigation water, increasing tolerance to salt stress. The more initial growth of maize is obtained in H 2 O 2 concentrations ranging from 7 to 8 µmol L -1 , being more efficient the application at sowing. The pretreatment of maize plants with H 2 O 2 from 15 µmol L -1 increasing stress, promoting further damage to vegetative growth.

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Section: Resultsunclassified

Section: Introductionunclassified

Métodos de aplicação de diferentes concentrações de H2O2 em milho sob estresse salino

Silva¹,

Lacerda²,

Medeiros³

et al. 2016

Revista Verde

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“…Nitrogen application at 120 kg ha −1 counteracted the damaging effects of salinity and notably improved growth, yield, and nitrogen uptake under salt stress (Gadalla et al 2007). In another study, nitrogen application above the recommended level for non-saline soils substantially increased Gondim et al (2010) Halopriming NaCl Earlier emergence and germination rate, plant height, yield Bakht et al (2011) Hormonal priming Ethylene Biomass Carvalho et al (2011) Halopriming NaCl Shoot dry weight and length, leaf number and area, chlorophyll Farahbakhsh and Saiid (2011) physiological traits such as leaf area index, crop growth rate, net assimilation rate, and all yield components in salt-stressed maize compared with the control (Akram et al 2010c). While nitrogen nutrition is important in crop production under salt stress, the nitrogen source is also important.…”

Section: Seed Priming and Nutrient Managementmentioning

confidence: 99%

Salt stress in maize: effects, resistance mechanisms, and management. A review

Farooq

Hussain

Wakeel

et al. 2015

Agron. Sustain. Dev.

564

383

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Maize is grown under a wide spectrum of soil and climatic conditions. Maize is moderately sensitive to salt stress; therefore, soil salinity is a serious threat to its production worldwide. Understanding maize response to salt stress and resistance mechanisms and overviewing management options may help to devise strategies for improved maize performance in saline environments. Here, we reviewed the effects, resistance mechanisms, and management of salt stress in maize. Our main conclusions are as follows: (1) germination and stand establishment are more sensitive to salt stress than later developmental stages. (2) High rhizosphere sodium and chloride decrease plant uptake of nitrogen, potassium, calcium, magnesium, and iron. (3) Reduced grain weight and number are responsible for low grain yield in maize under salt stress. Sink limitations and reduced acid invertase activity in developing grains is responsible for poor kernel setting under salt stress. (4) Exclusion of excessive sodium or its compartmentation into vacuoles is an important adaptive strategy for maize under salt stress. (5) Apoplastic acidification, required for cell wall extensibility, is an important indicator of salt resistance, but not essential for better maize growth under salt stress. (6) Upregulation of antioxidant defense genes and β-expansin proteins is important for salt resistance in maize. (7) Arbuscular mycorrhizal fungi improve salt resistance in maize due to better plant nutrient availability. (8) Seed priming is an effective approach for improving maize germination under salt stress. (9) Integration of screening, breeding and ion homeostasis mechanisms into a functional paradigm for the whole plant may help to enhance salt resistance in maize.

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“…In 6 day-old roots, the activity of G-POX was not significantly influenced with LTP treatment. Soaking of maize seeds with different concentration of hydrogen peroxide, which produced higher amount of oxidative stress also caused a decrease of G-POX activity but an increase of ascorbate peroxidase activity in comparison with a control seeds (Gondim et al 2010). On the other hand, the gamma irradiation treatment of chickpea seeds increased the activity of peroxidase two fold in comparison to control plants (Hameed et al 2008).…”

Section: Activity Of Antioxidant Enzymesmentioning

confidence: 99%

Growth, anatomy and enzyme activity changes in maize roots induced by treatment of seeds with low-temperature plasma

et al. 2012

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Abstract:The seeds of Zea mays L. cv. KWS were exposed to low-temperature plasma (LTP) by using Diffuse Coplanar Surface Barrier Discharge (DCSBD) for 60 and 120 seconds respectively. Growth parameters, anatomy of roots and activity of some enzymes (CAT, G-POX, SOD and DHO) isolated from roots grown from the seeds treated by LTP were evaluated. Our results indicate that LTP treatment of maize seeds affects post-germination growth of seedlings and this effect depends on the duration of LTP treatment. LTP treatment in duration of 60 seconds significantly increased the length, fresh and dry weight of the roots. However, the increase in time of LTP treatment to 120 seconds had inhibitive effect on the studied growth parameters. The activities of all studied antioxidant enzymes significantly increased with the age of maize seedlings in control conditions. On the other hand the application of LTP resulted in small, mostly non significant changes in the activity of antioxidant enzymes. Significant decrease in CAT activity was observed both in 3 and 6-day old maize roots and G-POX activity in 3-day old maize roots grown from seeds exposed to LTP for 60 seconds. A small, significant increase was detected only in SOD activity in 3-day old maize roots grown from seeds treated with LTP for 120 seconds and in 6-day old maize roots treated with LTP for 60 seconds. Significantly higher DHO activity was determined in embryos isolated from seeds treated with LTP for 60 seconds. On the contrary, in roots the DHO activity decreased with the time of LTP treatment. LTP treatment of seeds did not affect the anatomy of maize roots and caused only minor changes in the isoenzyme composition of G-POX and SOD.

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Pretreatment with H2O2 in maize seeds: effects on germination and seedling acclimation to salt stress

Cited by 54 publications

References 32 publications

Métodos de aplicação de diferentes concentrações de H2O2 em milho sob estresse salino

Métodos de aplicação de diferentes concentrações de H2O2 em milho sob estresse salino

Salt stress in maize: effects, resistance mechanisms, and management. A review

Growth, anatomy and enzyme activity changes in maize roots induced by treatment of seeds with low-temperature plasma

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