Effect of 28-homobrassinolide on growth, zinc metal uptake and antioxidative enzyme activities in Brassica juncea L. seedlings

Abstract

The present work was undertaken to study the effects of 28-homobrassinolide on growth, zinc metal uptake, antioxidative enzyme [guaiacol peroxidase (EC 1.11.1.7), catalase (EC 1.11.1.6), glutathione reductase (EC 1.6.4.2), ascorbate peroxidase (EC 1.11.1.11), superoxide dismutase (EC 1.15.1.1)] activities and protein content in 7-d-old seedlings of Brassica juncea L. treated with Zn metal at different concentrations. 28-homobrassinolide at 10 -9 M concentration lowered Zn uptake and bioconcentration factor. Different concentrations of Zn-metal treatment alone decreased the enzyme activities and protein concentration of seedlings. However, pre-sowing treatments of 28-homobrassinolide lowered the uptake of metal and enhanced the activities of enzymes and protein concentration of seedlings.

Effect of 28-homobrassinolide on antioxidant defence system in Raphanus sativus L. under chromium toxicity

“…Besides, increased uptake of Cr(VI) ions was remarkably lowered by 28-HBL treatments thereby resulted in mitigation of Cr metal toxicity in radish seedlings (Table 1). These results are in coherence with the reports of Sharma et al (2007). In B. juncea, Zn metal ions uptake was significantly lowered by 28-HBL treatments.…”

“…However, BRs are unique in their activities for not only regulating the diverse physiological and morphogenetic responses in plants but also having a significant role in amelioration of various biotic and abiotic stresses at nanomolar to micromolar concentrations (Krishna 2003;Clouse and Sasse 1998). Recently 28-HBL (10 -6 -10 -11 M) has been reported to combat salt and heavy metals stress in Brassica juncea, Raphanus sativus, Triticum aestivum and Zea mays Hayat et al 2007;Sharma et al 2007Sharma et al , 2010Yusuf et al 2010). Furthermore, 28-HBL is also observed to regulate the activities of various enzymes involved in photosynthesis and plant defence in wheat and Indian mustard exposed to different abiotic stresses (Hayat et al 2007;Yusuf et al 2010).…”

“…1). Previous reports also showed that exogenous application of BRs modified antioxidant enzyme activity in stressed maize, mustard, radish and wheat plants Sharma et al 2007Sharma et al , 2010Sharma et al , 2011Yusuf et al 2010). BRs (modified Sterols) can modulate the activity of proteins and other enzymes within the membrane by affecting either protein conformation (functionality) or protein activity by direct protein-sterol interactions (Lindsey et al 2003).…”

24-epibrassinolide regulated diminution of Cr metal toxicity in Brassica juncea L. plants

“…The level of these enzymes is increased by the action of 24-epiBL to overcome the stress generated by Cr and to enhance the resistance capacity of plants (Figure 1-3). This data is consistent with the studies that exogenous BRs treatment is effective in ameliorating stressful conditions rather than in optimal conditions (Sasse, 1997, Bhardwaj et al, 2007, Sharma and Bhardwaj, 2007bArora et al, 2008, Hayat et al, 2009. Similarly, Sharma et al, (2007) reported that pre-sowing treatments of 28-homobrassinolide lowered the uptake of metal and enhanced the activities of antioxidative enzymes and protein concentration of 7-d old B. juncea seedlings under zinc metal stress.…”

In VitroEffects of Brassinosteroids on the Growth and Antioxidant Enzyme Activities in Groundnut

“…It may be inferred from the enhancement in CAT activity that BR application modulated the antioxidative metabolism resulting in better growth of plants as suggested earlier [42,43]. An increase in POX activity in response to the application of BRs has also been reported [40,41,44]. The POX has been implicated in the synthesis of lignin and other phenolic polymers.…”