Primary metabolism components of seeds from Brazilian Amazon tree species

Gonçalves, José Francisco de Carvalho; Fernandes, Andréia Varmes; Oliveira, Antônio Fernando Morais de; Rodrigues, Lilian Figueiredo; Marenco, Ricardo Antonio

doi:10.1590/s1677-04202002000200009

Cited by 26 publications

(24 citation statements)

References 10 publications

(15 reference statements)

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“…In this work Parkia species seeds had protein contents ranging from 9.4% to 17.2%, another study with P. pendula seeds have revealed protein contents from 12.9% (Gonçalves et al 2002).…”

Section: Discussionmentioning

confidence: 62%

Partial purification of trypsin inhibitors from Parkia seeds (Fabaceae)

Chevreuil

Gonçalves

Calderon³

et al. 2014

Hoehnea

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-(Partial purification of trypsin inhibitors from Parkia seeds (Fabaceae)). Leguminous seeds (Fabaceae) have a high content of inhibitors of which serine protease inhibitors are the most widely studied. However, there are only a few studies related to the investigation of these proteins in tree species belonging to the Amazon flora. The protein content presented in seeds of four Amazonian Leguminosae species, Parkia pendula, P. discolor, P. multijuga and P. Nitida, was extracted by using NaCl 0.15 mol L -1 and then partially fractionated by using affinity chromatography performed on a trypsinSepharose 4B. These inhibitors presented different affinities between trypsin and chymotrypsin serine proteases, showing a higher inhibition to trypsin compared to chymotrypsin, except for P. nitida, which showed high inhibition against both enzymes. The SDS-PAGE analysis showed that the species from Parkia genus have a main band corresponding to partially purified trypsin inhibitors. The apparent molecular mass inhibitors (approximately 13-18 kDa) and the high specificity for trypsin suggest the occurrence of Bowman-Birk and Kunitz type inhibitors. Keywords: Amazonia, Bowman-Birk inhibitor, Kunitz inhibitor, leguminous seeds, serine protease RESUMO -(Purificação parcial de inibidores de tripsina de sementes de Parkia (Fabaceae)). Sementes de leguminosas (Fabaceae) apresentam alto conteúdo de inibidores, incluindo os inibidores de serinoproteinases que têm sido extensivamente estudados. Todavia, poucos estudos foram realizados quanto à investigação dessas proteínas em espécies arbóreas pertencentes à flora amazônica. As proteínas presentes nas sementes de quatro espécies de leguminosas da Amazônia, Parkia pendula, P. discolor, P. multijuga e P. nitida, foram obtidas pela extração usando NaCl 0.15 mol L -1 e, parcialmente purificadas usando a cromatografia de afinidade em tripsina-Sepharose 4B. Os inibidores exibiram afinidades diferentes entre a tripsina e a quimotripsina, exceto para P. nitida, a qual apresentou alta inibição contra as duas enzimas. A análise em SDS-PAGE mostrou que as espécies do gênero Parkia contém uma banda principal correspondendo aos inibidores de tripsina parcialmente purificados. As massas moleculares aparentes determinadas para os inibidores (aproximadamente, 13 a 18 kDa) e a alta especificidade pela tripsina sugerem a ocorrência de inibidores do tipo Bowman-Birk e Kunitz. Palavras-chave: Amazônia, Inibidor Bowman-Birk, Inibidor Kunitz, sementes de leguminosas, serinoproteinase 1.

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“…In this work Parkia species seeds had protein contents ranging from 9.4% to 17.2%, another study with P. pendula seeds have revealed protein contents from 12.9% (Gonçalves et al 2002).…”

Section: Discussionmentioning

confidence: 62%

Partial purification of trypsin inhibitors from Parkia seeds (Fabaceae)

Chevreuil

Gonçalves

Calderon³

et al. 2014

Hoehnea

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“…The nut kernel is known in the market for its high nutritional value, as it is rich in lipids, proteins, fibers, vitamins, and minerals, and especially selenium (CARDARELLI; OLIVEIRA, 2000;GONÇALVES et al, 2002;SOUZA;MENEZES, 2004). This last element has antioxidant activity and is, therefore, recommended in the prevention of cancer and cardiovascular diseases.…”

Section: Introductionmentioning

confidence: 99%

Repeatability of Fruits and Seeds Production and Selection of Brazil Nut Genotypes in Native Populations in Roraima1

Pedrozo¹,

Tonini

Resende

et al. 2015

Rev. Árvore

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-This study estimates the repeatability coefficients of two production traits in two native populations of Brazil nut trees. It determines the number of years of suitable evaluations for an efficient selection process, determines the permanent phenotypic correlation between production traits and also the selection of promising trees in these populations. Populations, located in the Itã region (ITA) and in the in the Cujubim region (CUJ), are both belonging to the municipality of Caracaraí, state of Roraima -Brazil, and consist of 85 and 51 adult trees, respectively. Each tree was evaluated regarding the number of fruits per plant (NFP) and fresh seed weight per plant (SWP), for eight (ITA) and five consecutive years (CUJ). Statistical analyses were performed according to the mixed model methodology, using Software Selegen-REML/BLUP (RESENDE, 2007). The repeatability coefficients were low for NFP (0.3145 and 0.3269 for ITA and CUJ, respectively) and also for SWP (0.2957 and 0.3436 for ITA and CUJ, respectively). It on average takes nine evaluation years to reach coefficients of determination higher than 80%. Permanent phenotypic correlation values higher than 0.95 were obtained for NFP and SWP in both populations. Although trees with a high number of fruits and seed weight were identified, more evaluation years are needed to perform the selection process more efficiently.Keywords: Bertholletia excelsa; Genetic improvement; Forest resources. REPETIBILIDADE DE PRODUÇÃO DE FRUTOS E SEMENTES E SELEÇÃO DE GENÓTIPOS DE CASTANHA-DO-BRASIL EM POPULAÇÕES NATIVAS EM RORAIMARESUMO -Os objetivos deste estudo foram estimar coeficientes de repetibilidade de dois caracteres de produção em duas populações nativas de castanheira-do-brasil, determinar o número de anos de avaliações adequado para um eficiente processo de seleção, determinar a correlação fenotípica permanente entre os caracteres de produção e selecionar árvores promissoras nessas populações. As populações, localizadas na região do Itã (ITA) e na região do Cujubim (CUJ), ambas pertencentes ao Município de Caracaraí, Estado de Roraima -Brasil, foram constituídas por 85 e 51 árvores adultas, respectivamente. Cada árvore foi avaliada quanto ao número de frutos por planta (NFP) e peso fresco de sementes por planta (SWP), por oito (ITA) e cinco anos consecutivos (CUJ). As análises estatísticas foram realizadas segundo a metodologia de modelos mistos, utilizando-se o Software Selegen-REML/BLUP (RESENDE, 2007 864Revista Árvore, Viçosa-MG, v.39, n.5, p.863-871, 2015 PEDROZO, C.A. et. al fenotípica permanente entre NFP e SWP acima de 0,95 foram obtidos nas duas populações. Apesar de ter sido possível identificar árvores com elevado número de frutos e peso de sementes, número maior de anos de avaliação é necessário para que o processo seletivo seja realizado de forma mais eficiente.

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“…Mobilization of seed food reserves may be related with the changes in seed vigor (CRANE et al, 2006). Therefore, information concerning how an embryo mobilizes its internal reserves during the early stage of germination can provide insights into the metabolic process of germination and consequently into the ability to use such seeds as planting material (GONÇALVES et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

“…Over the past decades, intensive harvest and high demand for the oil have threatened with extinction the remaining rosewood trees . Despite its economic importance, almost no information is available on seed germination of this tropical forest species and on thebiochemical routes involved in this process (PONTES et al, 2002;GONÇALVES et al, 2002). This investigation can help us to understand the biochemical aspects of rosewood seed germination that can be used to predict seed vigor.…”

Section: Introductionmentioning

confidence: 99%

Primary metabolite mobilization during germination in rosewood (Aniba rosaeodora Ducke) seeds

et al. 2008

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-This study aimed to characterize protein, oil, starch and soluble sugar mobilization as well as the activity of α-amylase during rosewood seed germination. Germination test was carried out at 25°C and the following parameters were analyzed: percentage of germination, initial, average, and final germination time. Seed reserve quantification was monitored in quiescent seeds and during different stages of radicle growth. Starch mobilization was studied in function of α-amylase activity. Germination reached 87.5% at the initial, average, and final time of 16, 21 and 30 days, respectively. Oil mobilization showed a negative linear behavior, decreasing 40% between the first and the last stage analyzed, whereas protein levels increased 34.7% during the initial period of germination. Starch content (46.4%) was the highest among those of the metabolites analyzed and starch mobilization occurred inversely to the observed for soluble sugars; α-amylase activity increased until the 15 th day, a period before radicle emission and corresponding to the highest starch mobilization. The high percentage of rosewood seed germination may be related to the controlled condition used in the germination chamber as well as to high seed reserve mobilization, in special oil and starch.Keywords: α-amylase, carbohydrates and oil. MOBILIZAÇÃO DE METABÓLITOS PRIMÁRIOS DURANTE A GERMINAÇÃO DE SEMENTES DE PAU-ROSA (Aniba rosaeodora Ducke)

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Primary metabolism components of seeds from Brazilian Amazon tree species

Cited by 26 publications

References 10 publications

Partial purification of trypsin inhibitors from Parkia seeds (Fabaceae)

Partial purification of trypsin inhibitors from Parkia seeds (Fabaceae)

Repeatability of Fruits and Seeds Production and Selection of Brazil Nut Genotypes in Native Populations in Roraima1

Primary metabolite mobilization during germination in rosewood (Aniba rosaeodora Ducke) seeds

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