Validação do sistema de transporte e das dosagens de amostras biológicas enviadas para a central de um laboratório de grande porte

Gabriel, Alexandre; Silva, Ana Amélia B.; Martino, Maria Cristina De; Razvickas, Wagner José; Silva, Regina Corrêa; Viana, Ana Maria; Maurício, Richard Couto; Santos, Silmara Aparecida Leopoldino; Tufik, Sérgio

doi:10.1590/s1676-24442007000400003

Cited by 3 publications

(4 citation statements)

References 3 publications

(4 reference statements)

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“…Limits for interpretation of the ICC and Kappa used in this study followed the parameters adopted by Gabriel et al 30 . ICC values greater than a score of 0.75 were considered good indicators of reliability, while values between 0.5 and 0.75 indicated a moderate to good reliability.…”

Section: Methodsmentioning

confidence: 99%

“…The parameters used for analyzes of the Kappa index (κ) were as follows: <0.40, poor agreement; κ between 0.40 and 0.60, reasonable or moderate agreement; κ between 0.60 and 0.80, good agreement; and κ above 0.80, excellent agreement 30 , 31 . Analyses were performed using Stata for Windows version 10.0 and Statistical Package for Social Sciences (SPSS), version 15.0.…”

Section: Methodsmentioning

confidence: 99%

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Brazilian version of the instrument of environmental assessment Craig Hospital Inventory of Environmental Factors (CHIEF): translation, cross-cultural adaptation and reliability

Furtado¹,

Sampaio²,

Vaz³

et al. 2014

Braz. J. Phys. Ther.

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Background: Environmental factors are essential for the characterization of human functioning and disability; however, the shortage of standardized instruments to assess environmental factors has limited the design of scientific investigations directed at identifying barriers to and facilitators of social participation of people with disabilities. Objectives: To translate to Brazilian Portuguese, cross-culturally adapt, and verify the reliability of an environmental assessment questionnaire, entitled Craig Hospital Inventory of Environmental Factors (CHIEF). Method: The questionnaire was translated to Portuguese, analyzed, translated back to English, and compared with the original version. The final version (CHIEF-BR) was submitted to 47 caregivers of children and adolescents with cerebral palsy (CP). The intra-rater reliability was tested using quadratic kappa and intraclass correlation coefficients (ICC), through interviews of 23 caregivers drawn from the total sample, on two occasions 10 days apart. Results: During submission of the questionnaires, it was observed that examples were needed in order to facilitate the understanding of the questions related to the politics sub-scale. Quadratic kappa showed that test-retest reliability of each question varied from 0.28 to 1.0 for the frequency score and from 0.30 to 0.98 for the magnitude score. Intraclass correlation coefficients for total scores showed high consistency indices (ICC≥0.92) for test-retest. Conclusion: The Brazilian version of the CHIEF was reproducible and applicable to the study sample. It may serve as an instrument to characterize the environmental barriers as well as a way to document the effects of interventions aimed at minimizing the impact of such barriers on the participation of children and adolescents with CP.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Brazilian version of the instrument of environmental assessment Craig Hospital Inventory of Environmental Factors (CHIEF): translation, cross-cultural adaptation and reliability

Furtado¹,

Sampaio²,

Vaz³

et al. 2014

Braz. J. Phys. Ther.

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“…During the RM shipment, samples are susceptible to the effects of temperature, length of time, mechanical shock, among other factors. Thus, it is remarkably important to assess the effects of the transportation system on the quality of laboratory results (12) . In some circumstances, it is not possible to maintain the appropriate conditions for assuring RM stability during shipment; in this case, it is recommended to take account of extra uncertainty related to the properties values (2) .…”

Section: Estabilidade Dos Soros Para O Controle De Qualidade Interno mentioning

confidence: 99%

Study on the stability of internal quality control sera for HIV/AIDS immunodiagnostic tests

Castejon¹,

Yamashiro²,

Oliveira³

et al. 2014

J. Bras. Patol. Med. Lab.

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Introduction: The use of reference materials in order to assure and perform the quality control of analytical measurements is a requirement in clinical laboratories. Objectives: Stability of serum samples, kept frozen at-20°C for long-term storage and at varied temperatures during short periods, was evaluated by investigating the persistency of anti-human immunodeficiency virus (HIV) antibodies reactivity on enzyme-linked immunosorbent assay/enzyme immunoassay (ELISA/EIA), Western blot and indirect immunofluorescence assays. Method: The analyzed sera were part of serum panels (comprised of anti-HIV positive and negative samples), produced at the Immunology Center of Instituto Adolfo Lutz, which have been the reference specimens for producing the internal quality assurance sera of HIV/acquired immunodeficiency syndrome (AIDS) immunodiagnostic assays. Sera stability was assessed in samples stored at-20°C for 56 weeks, and at various temperature conditions: from 2°C to 8°C (refrigerator), from 15°C to 25°C (room temperature), at 37°C (incubator) and at-80°C (freezer) for 24 and 48 hours. The statistical analyses on HIV-negative serum samples (long-term storage) were significant (p < 0.05), and neither adverse effects on these samples as the occurrence of false-positive results nor false-negative results in HIV antibody positive sera were found in both studies. Conclusion: It was possible to conclude that the reference material remained stable for 48 hours at different temperatures (short-term) and it remained stable at-20°C for 56 weeks (long-term).

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“…Ainda assim, havendo a necessidade, a adoção de controle rígido sobre as condições de transporte pode ser um fator crucial para um laudo correto. Um estudo de validação feito por um laboratório em Jundiaí no estado de São Paulo evidenciou que o controle das condições de transporte eleva consideravelmente a qualidade do resultado, gerando concordância de 94%, 87,5% e 88% para hematúria, leucocitúria e urocultura respectivamente, em relação a amostra analisada no laboratório local e as amostras transportadas para outros laboratórios (GABRIEL et al, 2007).…”

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Ambiente Laboratorial E Controle De Qualidade: Uma Atenção Aos Erros Pré-Analiticos Mais Frequentes

Barbosa¹,

Silva²,

Costa³

et al. 2021

Revista Científica Multidisciplinar Núcleo do Conhecimento

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A fase pré-analítica é a primeira fase a ser seguida para execução de exames laboratoriais. Sendo uma das fases cruciais, cuidados especiais são exigidos para evitar erros que interfiram no diagnóstico conclusivo do paciente. Como objetivo principal apresentam-se os erros laboratoriais mais frequentes e suas consequências em todo o processo de diagnóstico de pacientes, com ênfase nas falhas da fase pré-analítica. Os laboratórios devem seguir padrões estabelecidos previamente por órgãos responsáveis pela fiscalização de qualidade e desempenho dos mesmos, como a ANVISA (Agência Nacional de Vigilância Sanitária) e resoluções de critérios que devem ser seguidos na rotina laboratorial, como a RDC 302/2005. Apesar de toda essa fiscalização, ainda há muitas falhas nos processos analíticos, principalmente na fase pré-analítica, onde ocorrem os erros mais comuns e constantes nos laboratórios, o que acarreta danos aos laboratórios e aos pacientes. Mesmo com todas as instruções, os erros mais corriqueiros acontecem em setores que não são automatizados, ou seja, que dependem especialmente da interferência humana, como a omissão de dados importantes do paciente para com o laboratório, a falta de informações que devem ser passadas aos pacientes antes da coleta das amostras, erros no momento da coleta, do transporte e preparação inicial. Esta revisão de literatura foi produzida através de buscas em revistas eletrônicas nas bases de dados Scientific Eletronic Library Online (SciELO), Google Scholar e PubMed, em artigos publicados entre os anos de 2010 a 2020. Concluindo, torna-se necessário a implementação de medidas que corrijam os erros que mais ocorrem na fase pré-analítica dentro do ambiente laboratorial, desacertos estes que devem ser analisados dia após dia por programas de controle de qualidade contratados pelos laboratórios responsáveis, visando sanar todos estes erros, trazendo assim qualidade nos exames e uma melhor decisão nos tratamentos dos respectivos pacientes.

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Validação do sistema de transporte e das dosagens de amostras biológicas enviadas para a central de um laboratório de grande porte

Cited by 3 publications

References 3 publications

Brazilian version of the instrument of environmental assessment Craig Hospital Inventory of Environmental Factors (CHIEF): translation, cross-cultural adaptation and reliability

Brazilian version of the instrument of environmental assessment Craig Hospital Inventory of Environmental Factors (CHIEF): translation, cross-cultural adaptation and reliability

Study on the stability of internal quality control sera for HIV/AIDS immunodiagnostic tests

Ambiente Laboratorial E Controle De Qualidade: Uma Atenção Aos Erros Pré-Analiticos Mais Frequentes

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