Construção de arrays de tecido com equipamento alternativo e de baixo custo para estudo imuno-histoquímico de tumores mamários

Andrade, Victor Piana de; Nunes, Cristiana Buzelin; Rocha, Gislene Fátima Silva; Sanches, Fernanda Squárcio Fernandes; Oliveira, Flávio Nepomuceno; Gobbi, Helenice

doi:10.1590/s1676-24442006000600012

Cited by 9 publications

(9 citation statements)

References 5 publications

(5 reference statements)

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“…With 60-day treatment, the SSFR reduction was not significantly different than the control group. Studies have shown that the prolonged use of psychotropics causes alterations in receptor sensitivity 24. In addition, therapeutic effects and side effects gradually decrease after continuous or repeated psychotropic administration, which may cause desensitization, resistance, or tolerance.…”

Section: Discussionmentioning

confidence: 99%

The effects of antidepressants and pilocarpine on rat parotid glands: an immunohistochemical study

Mattioli

Silva

Grégio

et al. 2011

Clinics

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OBJECTIVES:To evaluate the effects of antidepressants and pilocarpine on the quantity of myoepithelial cells and on the proliferation index of the epithelial cells of rat parotid glands.INTRODUCTION:Hyposalivation, xerostomia, and alterations in saliva composition are important clinical side effects related to the use of antidepressants.METHODS:Ninety male Wistar rats were allocated to nine groups. The control groups received saline for 30 (group C30) or 60 days (group C60) or pilocarpine for 60 days (group Pilo). The experimental groups were administered fluoxetine (group F30) or venlafaxine for 30 days (group V30); fluoxetine (group FS60) or venlafaxine (group VS60) with saline for 60 days; or fluoxetine (group FP60) or venlafaxine (group VP60) with pilocarpine for 60 days. Parotid gland specimens were processed, and the immunohistochemical expression of calponin and proliferating cell nuclear anti-antigen on the myoepithelial and parenchymal cells, respectively, was evaluated. Analysis of variance (ANOVA), Tukey HSD and Games-Howell tests were applied to detect differences among groups (p<0.05).RESULTS:Compared with the controls, chronic exposure to antidepressants was associated with an increase in the number of positively stained cells for calponin. In addition, venlafaxine administration for 30 days was associated with an increase in the number of positively stained cells for proliferating cell nuclear anti-antigen. Fluoxetine and pilocarpine (group FP60) induced a significant decrease in the number of positively stained cells for calponin compared with all other groups.CONCLUSIONS:The number of positively stained cells for calponin increased after chronic administration of antidepressants. The proliferation index of the epithelial cells of rat parotid glands was not altered by the use of antidepressants for 60 days.

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Section: Discussionmentioning

confidence: 99%

The effects of antidepressants and pilocarpine on rat parotid glands: an immunohistochemical study

Mattioli

Silva

Grégio

et al. 2011

Clinics

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“…Das técnicas de TMA encontradas na literatura, aquelas que possuem maior semelhança com a combinação 6 foram as propostas por Rocha et al (17) e por Kononen et al (12) (a técnica original). A similaridade entre as três técnicas consiste em todas utilizarem o mecanismo de agulhas com êmbolo para punção e bloco receptor, em cujas perfurações são depositadas as amostras puncionadas com o movimento do êmbolo.…”

Section: Combinações 6 Eunclassified

“…A similaridade entre as três técnicas consiste em todas utilizarem o mecanismo de agulhas com êmbolo para punção e bloco receptor, em cujas perfurações são depositadas as amostras puncionadas com o movimento do êmbolo. Entretanto, enquanto neste trabalho propomos a construção de blocos com até 72 amostras para essa combinação, a técnica original de TMA (12) possibilita a confecção de blocos com até 1 mil amostras e, no estudo de Rocha et al (17) , a construção de blocos de até 55 amostras. O custo da técnica proposta por Rocha et al (17) (US$ 180) foi menor em relação ao custo da combinação 6 (US$ 203,94).…”

Section: Combinações 6 Eunclassified

“…Entretanto, enquanto neste trabalho propomos a construção de blocos com até 72 amostras para essa combinação, a técnica original de TMA (12) possibilita a confecção de blocos com até 1 mil amostras e, no estudo de Rocha et al (17) , a construção de blocos de até 55 amostras. O custo da técnica proposta por Rocha et al (17) (US$ 180) foi menor em relação ao custo da combinação 6 (US$ 203,94). A confecção dos blocos receptores no presente estudo diferiu em relação à confecção de Kononen et al (12) e Rocha et al (17) ; enquanto ambas as técnicas utilizam o mesmo mecanismo de perfuração no bloco de parafina, orientando-se por coordenadas precisas para a obtenção de uma matriz de perfurações uniforme e bem alinhada, nas combinações 6 e 8, as perfurações foram realizadas com grande facilidade por meio da placa de alumínio perfurada e da agulha hipodérmica modificada.…”

Section: Combinações 6 Eunclassified

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Avaliação de combinações de técnicas alternativas de construção e montagem de blocos de tissue microarray

Santos¹,

Rego²,

Sobral

2010

J. Bras. Patol. Med. Lab.

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“…Our results showed that the rabbit antibodies could be used in higher working dilutions when using antigen retrieval (Figure 1). They represent an alternative for estrogen and progesterone receptor evaluation in clinical tests (44,45) . (1,23) .…”

Section: Er and Pr Antibodiesmentioning

confidence: 99%

Predictive factors of breast cancer evaluated by immunohistochemistry

Gobbi¹,

Nunes²

2008

J. Bras. Patol. Med. Lab.

Self Cite

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Hormone receptor and Her2 protein overexpression evaluated by immunohistochemistry (IHC) is widely validated as a predictive factor in breast cancer. The quality of the IHC reaction is influenced by tissue fixation and processing. Over-and underfixation deeply affect IHC results. Antigen retrieval may improve IHC but it does not recover tissue from autolysis or overfixation. The choice of primary antibody for IHC as to its sensitivity and specificity in relation to therapeutic response represents an important stage. Apart from mouse monoclonal antibodies, new rabbit monoclonal antibodies are commercially available, such as clones anti-ER SP1 and B644, anti-PR SP2 and B645 and anti-Her2 SP3 and 4B5. They represent an alternative to hormone receptor and Her2 evaluation by IHC. New polymeric nonbiotinylated detection systems are also available and allow accurate and strong marking with no stromal and no non-specific cytoplasmic staining due to endogenous biotin. The most recommended cut off for estrogen and progesterone receptors (ER and PR) is more than 1% of positive cells with moderate or strong staining intensity (Allred's scoring system). New guidelines for Her2 evaluation by IHC show a cut off of more than 30% of positive cells with strong intensity (3+) that correlates better with gene amplification. The 2+ cases are now considered indeterminate and should be confirmed by fluorescence in situ hybridisation (FISH)

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Construção de arrays de tecido com equipamento alternativo e de baixo custo para estudo imuno-histoquímico de tumores mamários

Abstract: Construção de arrays de tecido com equipamento alternativo e de baixo custo para estudo imuno-histoquímico de tumores mamários Construction of low cost tissue microarrays using alternative equipment for immunohistochemical study of breast tumors

Cited by 9 publications

References 5 publications

The effects of antidepressants and pilocarpine on rat parotid glands: an immunohistochemical study

The effects of antidepressants and pilocarpine on rat parotid glands: an immunohistochemical study

Avaliação de combinações de técnicas alternativas de construção e montagem de blocos de tissue microarray

Predictive factors of breast cancer evaluated by immunohistochemistry

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