Altered lectin-binding sites in normal colon and ulcerative colitis

Melo-Júnior, Mário Ribeiro de; Telles, Adriana Maria da Silva; Albuquerque, Francisco E. B.; Pontes-Filho, Nicodemos Teles de; Carvalho, Luiz Bezerra de; Beltrão, Eduardo Isidoro Carneiro

doi:10.1590/s1676-24442004000200011

Cited by 12 publications

(10 citation statements)

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“…Quantitative image analysis of the number and area of cells confirmed that the histological differentiation in tumours seem to be also related to changes in the expression of surface carbohydrates in cells (21,25,34) . Aberrant glycosylation of tumour cells, defined by the expression of tumour associated carbohydrate antigens (TACAs), is even more frequently found in tumours than activation of certain oncogenes, such as ras.…”

Section: Image Analysis Data Of Lectin Histochemistry In the Areamentioning

confidence: 76%

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Digital image analysis of skin neoplasms evaluated by lectin histochemistry: potential marker to biochemical alterations and tumour differential diagnosis

Melo-Júnior¹,

Araújo-Filho²,

Patu³

et al. 2006

J. Bras. Patol. Med. Lab.

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Section: Image Analysis Data Of Lectin Histochemistry In the Areamentioning

confidence: 76%

“…Such methods include colorimetric analysis of neoplasic cell culture (1) , automatic cytometry (28) , nuclear morphometric variation analysis (35) and image analysis of tumour lectin staining (22,25) .…”

Section: Introductionmentioning

confidence: 99%

Digital image analysis of skin neoplasms evaluated by lectin histochemistry: potential marker to biochemical alterations and tumour differential diagnosis

Melo-Júnior¹,

Araújo-Filho²,

Patu³

et al. 2006

J. Bras. Patol. Med. Lab.

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“…Experimental evidences suggest that sugars of the cell surface are involved in the processes of recognition process (Melo-Junior et al, 2004;Melo-Junior et al, 2006a;2006b;Araújo-Filho et al, 2006) indicating an important contribution of lectins in those biomedical analysis.…”

Section: Discussionmentioning

confidence: 99%

Carbohydrates Detection in the Hepatic Egg - Granuloma System Using Lectin Histochemistry

et al. 2008

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MELO-JÚNIOR, M. R.; CAVALCANTI, C. L. B.; DE PONTES-FILHO, N. T.; DE CARVALHO JR, L. B. & BELTRÃO, E. I. C.Carbohydrates detection in the hepatic egg-granuloma system using lectin histochemistry. Int. J. Morphol.,26(4):967-972, 2008. SUMMARY:This study aims to evaluate the egg-granuloma system in hepatic tissues using lectin histochemistry in experimental Schistosomiasis. Eight Swiss mice were infected with a local strain of Schistosoma mansoni, being submitted forty days later to a perfusion after which slices of liver (4µ m) were prepared. The tissue samples were incubated with the following peroxidase conjugated lectins: Peanut agglutinin (PNA), Wheat Germ agglutinin (WGA), and Concanavalin A (Con A). All lectins recognized the glycoconjugates in the adult worm tegument. In the hepatic tissue, WGA presented the highest staining followed by PNA and Con A. The PNA presented the most intense staining of the egg-granuloma system while WGA stained the hepatic sinusoid cells and Con A bound preferentially the fibrosis rings of granuloma and the surrounding hepatic parenquima. WGA and PNA indicated the presence of residues of N-acetyl-glucosamine and galactose in the surface of Schistosoma mansoni eggs in the hepatic granulomas. In conclusion, using PNA, Con A and WGA our study presented different aspects of the egg-granuloma and Tegument of Schistosoma mansoni as well as indicated differences in the peri-ovular granulomas indicating alterations in the cellular mechanism of expression of surface carbohydrates during progression of the Schistosomiasis.

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“…Other lectins used in this study like wheat germ agglutinin (WGA), soy bean (SBA), grifonia seed (GSI) showed similar affinity to normal, UC and CD biopsies. In the study of Melo-Junior et al (Melo-Junior et al, 2004), it was found that WGA presented recognition pattern for diseased tissue. The authors claim that Nacetylglucosamine was absent or not accessible for lectin recognition in normal tissues, as well as mannosides and galactose.…”

Section: Specific Colon Targetingmentioning

confidence: 98%