Diagnóstico laboratorial da infecção pela Chlamydia trachomatis: vantagens e desvantagens das técnicas

Seadi, Claudete Farina; Oravec, Rejane Maria; Poser, Beatriz von; Cantarelli, Vlademir Vicente; Rossetti, Maria Lúcia Rosa

doi:10.1590/s1676-24442002000200009

Cited by 13 publications

(27 citation statements)

References 28 publications

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“…The main disadvantage is the necessity of an expensive laboratorial infrastructure; it is also laborious and demands careful handling to maintain the microorganisms viable. However, this is compensated by 100% specificity and 80% sensitivity [12]. In our study the prevalence of Chlamydia infection among the pregnant women, determined by cell culture, was 8.0%, similar to literature values [7,17,18].…”

Section: Discussionsupporting

confidence: 90%

“…Endocervical and/or metaplasical cell frequency in samples taken with Ayre's spatula and a cytobrush used cell culture as a gold standard for Chlamydia infection. Advantages of cell culture include low probability of contamination and preservation of the microorganisms for future study [12]. The main disadvantage is the necessity of an expensive laboratorial infrastructure; it is also laborious and demands careful handling to maintain the microorganisms viable.…”

Section: Discussionmentioning

confidence: 99%

“…The available laboratorial tests for the detection of Chlamydia include McCoy cell culture, direct immunofluorescence, enzymatic immunoassay, hybrid capture, and polymerase chain reaction (PCR); the latter gives the greatest sensitivity. Indirect diagnosis tests, such as a search for specific antibodies and Papanicolaou staining, though they have low sensitivity, are often the only available examination for many women [12,13]. The culture technique, which is a diagnostic method with high sensitivity and specificity (80% and 100%, respectively), is costly, time consuming, and requires considerable expertise, which limits its use in large healthcare centers [12].…”

mentioning

confidence: 99%

“…Indirect diagnosis tests, such as a search for specific antibodies and Papanicolaou staining, though they have low sensitivity, are often the only available examination for many women [12,13]. The culture technique, which is a diagnostic method with high sensitivity and specificity (80% and 100%, respectively), is costly, time consuming, and requires considerable expertise, which limits its use in large healthcare centers [12]. The Papanicolaou stain, well established for the detection of pre-cancer cervix lesions, has been used as alternative method for the diagnosis of the C. trachomatis [14].…”

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confidence: 99%

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Efficacy of cytology for the diagnosis of Chlamydia trachomatis in pregnant women

Cornetta¹,

Gonçalves²,

Bertini³

2006

Braz J Infect Dis

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This study evaluated the effectiveness of Papanicolaou staining for the initial diagnosis of Chlamydial infection in pregnant women. A hundred thirteen patients were examined with a Papanicolaou test, independent of gestational age, parity or maternal age. Three endocervical samples were collected; the first two were collected with a brush (Cytobrush plus, Mediscand, Sweden) and the third with Ayre's spatula. The first specimen was used for McCoy cell culture and the other two were examined cytologically. Chlamydial infection was detected in 9 (7.9%) patients. Only one (0.8%) was diagnosed by cytological exam. The sensitivity and specificity of the cytological examination were 10 and 98%, respectively. The estimated positive predictive value was 33.3% and the negative predictive value was 92.7%. When Papanicolaou stain diagnosis suggests Chlamydia, a more specific complementary exam should be added to confirm infection; subsequently adequate treatment can be implemented, thereby preventing the frequent complications of untreated subclinical infections.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Efficacy of cytology for the diagnosis of Chlamydia trachomatis in pregnant women

Cornetta¹,

Gonçalves²,

Bertini³

2006

Braz J Infect Dis

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“…Cytological analysis for the detection of Chlamydia trachomatis, available since 1907, was the first method used in cell samples and inclusion conjunctivitis secretions in infants (1,6) . Other methods, such as complement fixation test, cell culture, and hybrid capture, were subsequently developed, and the last two show high sensitivity and specificity (16)(17)(18)(19) . Nowadays, modern laboratory tests are available, using immunological and molecular techniques based on nucleic acid amplification, such as polymerase chain reaction (PCR) (16)(17)(18)(19) .…”

Section: Introductionmentioning

confidence: 99%

Detection of chlamydia trachomatis by immunological methods in adult and adolescent female population in Cuiabá, Mato Grosso

Matos¹,

Machado²,

Zavala³

et al. 2015

J Bras Doenças Sex Transm

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ARTICLE INTRODUCTIONThe WHO estimates that there are 105.7 million new cases of Chlamydia worldwide, with 3 to 4 million of these only in the United States of America (USA), with over 1.4 million cases reported in 2011 (1)(2)(3)(4)(5) , 5 million in Eastern Europe, and 34 million in subSaharan Africa and southeast Asia (5)(6)(7)(8)(9) . Worldwide, it occurs more frequently in sexually active young adults, generally aged younger than 20 years, and it is nearly three times higher in the 14-24 years age group (5,7,9,10) . It currently represents the leading cause of female infertility, but the most common infections are urethritis and cervicitis, which, if untreated, can lead to serious consequences in the reproductive tract such as ectopic pregnancy and infertility (6,(8)(9)(10) . About 50% of infected men and 70 to 75% of infected women are asymptomatic. The infection is transmitted during sexual contact and to the newborn at birth and may cause neonatal conjunctivitis or pneumonia (5,6,(8)(9)(10) . Over 100 million individuals of both sexes worldwide are infected with Chlamydia at some point in their lives (6,9,10) . Among male subjects, the prevalence is comparable with that of risk populations, ranging from 15 to 20%, while in the asymptomatic population, it is between 3 and 7%. In female subjects, the infection rates of asymptomatic populations are between 3 and 10%, while in high-risk populations they are above 20% (1)

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Simultaneous Chlamydia trachomatis and HPV infection in pregnant women

Pereira

Etlinger

Aguiar

et al. 2009

Diagnostic Cytopathology

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Pregnancy is associated with HPV infection and with Chlamydia trachomatis (CT) infection mostly due to the natural immunosuppression. In addition, pregnancy associated to CT infection can lead to adverse conditions to the woman and fetus, and CT is also believed to be a co-factor in human immunodeficiency virus infection and HPV-induced cervical cancer. The aim of this study was to establish the odds ratios (OR) of CT infection in to HPV-infected pregnant women and vice versa of women stratified by age (<25 years) and marital status. This work is part of a national multicentric transversal study carried out in six Brazilian cities supported by the Ministry of Health of Federal Government of Brazil in 2003. Cervical scrapes of 371 pregnant women were sampled. We performed a hybrid capture-2 technique to diagnose these samples on HPV and CT infection, and the women responded a questionnaire. Significant association was observed between nonstable marital status and hr-HPV infection [OR = 2.61 (1.38-4.97) P = 0.003)], and age <25 years old [OR = 2.26 (1.09-4.71) P = 0.029]. Nonstable marital status was also associated with lr-HPV infection [OR = 2.67 (1.59-4.50) P < 0.001), and age <25 years old [OR = 2.55 (1.51-4.32) P < 0.001). Fifty of the 371 pregnant women were infected with hr-HPV (13.5%) and 111 (30.0%) were infected with lr-HPV. The coinfections of HPV and CT were found in 31 women, that is, 8.36% of the pregnant women (P < 0.001). The high rate of simultaneous CT and HPV infection in pregnant women favors the recommendation to screen pregnant women for both CT and HPV.

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Diagnóstico laboratorial da infecção pela Chlamydia trachomatis: vantagens e desvantagens das técnicas

Cited by 13 publications

References 28 publications

Efficacy of cytology for the diagnosis of Chlamydia trachomatis in pregnant women

Efficacy of cytology for the diagnosis of Chlamydia trachomatis in pregnant women

Detection of chlamydia trachomatis by immunological methods in adult and adolescent female population in Cuiabá, Mato Grosso

Simultaneous Chlamydia trachomatis and HPV infection in pregnant women

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