Estimativa da variabilidade genética intra-específica da dourada - Brachyplatystoma rousseauxii Castelnau 1855 (Pimelodidae - Siluriformes) no sistema Estuário-Amazonas-Solimões

Abstract

The dourada (Brachyplatystoma rousseauxii) is among the two most important catfish species in the Amazon, being captured by both artisanal and commercial fishing fleets from Iquitos (Peru) to the estuary of the Amazonas river, in Belém. Studies by several authors suggest that the species has differentiated areas for feeding/growth and reproduction, and that, in the Amazon, it is composed by a single population that

Genetic diversity and population structure of bocachico Prochilodus magdalenae (Pisces, Prochilodontidae) in the Magdalena River basin and its tributaries, Colombia

“…The present study demonstrates that P. magdalenae has a high genetic diversity, He = 0.877, compared to that observed for other species of the genus (Santacruz, 2003; Hatanaka et al , 2006; Galzerani, 2007; Carvalho-Costa et al , 2008; Silva, 2011; Rueda et al , 2011) and to other commercially important species that have the same reproductive pattern and migrate long distances (Sanches, 2007; Batista, 2010; Dantas, 2010) (Table 4). Likewise, the microsatellite genetic diversity was slightly lower than that reported by Aguirre et al (2013) for mitochondrial DNA (mtDNA control region) in P. magdalenae (HD = 0.997).…”

Genetic structure and historical diversification of catfish Brachyplatystoma platynemum (Siluriformes: Pimelodidae) in the Amazon basin with implications for its conservation

“…Heterologous amplifications were performed using the microsatellite loci and PCR conditions developed by Batista ( 2010 ). PCR was performed in 10 μ L reaction mixture containing 2 μ L DNA template (10–50 ng/ μ L), 1.0 μ L 10× PCR buffer (10 mmol/L tris-HCl, 50 mmol/L KCl, pH 8.4), 0.5 μ L forward primer (5 μ mol/L), 1.0 μ L primer R (5 μ mol/L), and 0.5 μ L primer M13 labeled at the 5′ with FAM, HEX, or NED fluorescent dyes, 2.1 μ L dNTPs (1 mmol/L), 0.30 μ L MgCl 2 (50 mmol/L), and 0.21 μ L of Taq DNA polymerase 1U/ μ L. The amplification of loci was performed using the following thermocycling conditions: initial denaturation at 92°C for 1 min, followed by 25 cycles at 94°C for 20 sec; 60–62°C for 40 sec; 68°C for 35 sec, followed by annealing of the primer using fluorescence 20 cycles at 93°C for 20 sec; and 53°C for 30 sec an extension to this cycle at 72°C for 35 sec.…”

ESTUDIO COMPARATIVO DE LA VARIABILIDAD GENÉTICA DEL ZÚNGARO TIGRINUS - Brachyplatystoma tigrinum (Britski, 1981) Y DOS ESPECIES RELACIONADAS DE LA FAMILIA PIMELODIDAE

“…7 4 % respectivamente). La composición de bases nucleotídicas encontradas para las tres especies estudiadas, fue similar a las reportada por Batista (2001), Huergo (2009) (Brown et al, 1993;Faber & Stepien, 1997;Schneider, 2007;Chiang et al, 2008). Esta tendencia concuerda con Meyer (1993), que explica que la región control es altamente variable y es común encontrar zonas con elevada frecuencia de A/T, porque estas son más estables que las zonas con C/G.…”