Physical evaluation, morphological and identification of seminal proteins in Santa Ines sheep

Abstract

SUMMARY This study aimed to identify proteins in the seminal plasma associated with fertility in sheep of Santa Inês in Manaus, AM, using twodimensional electrophoresis techniques associated with mass spectrometry. Semen samples from eight adult sheep were collected by removing an aliquot for the physical and morphological assessments of semen and seminal plasma was subjected to SDS-PAGE profile and two-dimensional electrophoresis. Gels were stained with colloidal Coomassie, scanned and analyzed using ImageMaster 2D Platinum software, version 6.0. The selected individual spots were cut from the master gel, digested with trypsin and subjected to identification by mass spectrometry (MALDITof / Tof). Of the 108 spots detected in the gel, it selected 10 differential spots (based on the distribution thereof in the bidimensional gel and pre-analysis of the 2D ImageMaster Platinum Software) identifying 03 proteins: clusterin, a protein 14-3-3 zeta chain and Ram Seminal versicles 22kDa Protein. The identity of these proteins implies that the components of seminal plasma participate in physiological processes involved in sperm protection, motility and sperm capacitation, all associated with fertility. These proteins need to be better studied to see whether the same could be used as molecular markers of fertility as they were also found in other studies conducted with sheep Santa Ines.

Effect of quercetin or butylated hydroxytoluene on cooled or frozen-thawed ram sperm quality

“…The overall ejaculated volume, sperm concentration, total sperm ejaculated, and sperm motility of the rams used in this study were consistent with those of other studies on Santa Ines and Texel sheep breeds (Oliveira et al, 2017;Mandiki et al, 1998). The inclusion of quercetin or BHT in the semen with a freezing egg yolk-based extender was tested for sperm motility and viability of either cooled or frozen-thawed ram semen.…”

“…The study was conducted from March to June 2015. Thirty rams (15 Santa Inês breed and 15 Texel breed) ranging from 2 to 5 years old, clinically healthy, with a good body condition score and satisfactory sperm parameters (Table 1) were enrolled in this study (Oliveira, Oliveira, Lima, Andrade, Abreu, & Oliveira, 2017;Mandiki, Derycke Bister, & Paquay, 1988). The rams were kept on a farm in Iaras city, SP,Brazil (22°52'15'' S 49°09'46'' W), and a total of 178 ejaculates were harvested and divided into two experiments, as described below.…”