Resíduo da extração de própolis como inibidor bacteriano in vitro

Abstract

RESUMO Objetivou-se avaliar o efeito dos resíduos da extração hidroalcoólica de própolis dos tipos verde e marrom sobre o desenvolvimento de bactérias Gram-positivas e Gram-negativas. As própolis brutas foram adquiridas da apicultura “Companhia da Abelha”, instalada em Contagem, Minas Gerais. A própolis verde foi derivada de “alecrim-do-campo” (Baccharis dracunculifolia), e a própolis marrom foi derivada de “alecrim-do-campo” (B. dracunculifolia) e “assa-peixe” (Vernonia polyanthes). Foram usados cinco níveis de inclusão (0; 0,625; 0,125; 0,25; 0,5g) dos resíduos, além do controle para o inóculo (CI), e seis amostras bacterianas, sendo duas delas Gram-positivas e quatro Gram-negativas. O método utilizado foi o de contagem de unidades formadoras de colônias em placas de petri (pour plate). Entre as bactérias Gram-positivas, o maior efeito inibidor foi detectado para amostras de Staphylococcus aureus, e maior susceptibilidade foi para a Escherichia coli dentre as Gram-negativas. Houve efeito antimicrobiano do resíduo da extração da própolis verde sobre as bactérias S. aureus e S. intermedius. O resíduo da extração da própolis marrom apresentou maior inibição para S. intermedius somente nos tratamentos com 0,5 e 0,25g de resíduo. Não houve efeito do resíduo da extração de própolis verde ou marrom sobre o crescimento de bactérias gênero Pseudomonas. Os resíduos da extração hidroalcoólica de própolis verde e marrom atuaram como inibidores de crescimento das bactérias Staphylococcus aureus, Staphylococcus intermedius e Escherichia coli, e em menor grau para Salmonella e Klebsiella. O resíduo tem maior efeito inibidor em bactérias Gram-positivas.

Metal Content of Nutritional and Toxic Value in Different Types of Brazilian Propolis

“…One of the sources of propolis contamination is the plants in which bees collect the resins. Divan et al [35] showed that Baccharis dracunculifolia, one of the sources of resin collection for the production of brown propolis [23], has a moderate capacity to accumulate Cd, transforming this plant into a possible source of contamination. e presence of Cd in this sample may be related to the use of agricultural supplies, such as fungicides, in plantations in Santa Catarina state [34].…”

Application of propolis in agriculture

“…Among these groups, the green and red propolis are known all over the world and have been extensively researched. There is also a type of propolis called geopropolis found in South America, which peculiar characteristic is the fact that stingless bees incorporate soil when producing it (BARTH, 2006;SILVA et al, 2016).…”

Evaluation of the residues from the ethanolic extraction of organic propolis as a source of biological compounds

“…According to Mirzoeva et al, (1997), propolis had bacteriostatic action on Gram-positive and some Gramnegative bacteria. Propolis apparently modifies the bioenergetics status of bacterial membrane and inhibits bacterial motility (HEIMBACH et al, 2016). Moreover, propolis acts as a bactericidal agent, stopping division of bacterial cell, destroying the cell wall and stopping the protein synthesis (LOTFY, 2006;PAROLIA et al, 2010;MACHADO et al, 2016;ANJUM et al, 2018).…”

“…After the ethanolic extraction of crude propolis, the ethanolic solution have a high market value being used as a final dosage form or occasionally incorporated into foods, beverages, medicines or cosmetics. However, the resinous part constitutes the waste material of propolis, which represents almost 80-94% of propolis used to obtain the extract, being composed of several substances such as wax, resins and gums HEIMBACH et al, 2016;.Nevertheless, it cannot be use directly on food industry due to its resinous composition REIS et al, 2017;.…”

“…Propolis is a resinous product made by Apis mellifera L. bees, which is used to protect the hive against insects and microorganisms TRUSHEVA, 2014;FERREIRA et al, 2017;KAMATOU et al, 2019). The most used form of propolis is the ethanolic extract (EEP), but a resinous compound constituted of several substances such as wax, resins and gums is also obtained as a residue, which represents almost 80-94% of propolis HEIMBACH et al, 2016;. The use of these residues in animal feed may contribute to the reduction of wastes, dealing with the current environmental concern, as well as provide valuable nutrients at a low cost (SANTOS et al 2013;REIS et al, 2017).…”