RESUMO Objetivou-se avaliar o efeito dos resíduos da extração hidroalcoólica de própolis dos tipos verde e marrom sobre o desenvolvimento de bactérias Gram-positivas e Gram-negativas. As própolis brutas foram adquiridas da apicultura “Companhia da Abelha”, instalada em Contagem, Minas Gerais. A própolis verde foi derivada de “alecrim-do-campo” (Baccharis dracunculifolia), e a própolis marrom foi derivada de “alecrim-do-campo” (B. dracunculifolia) e “assa-peixe” (Vernonia polyanthes). Foram usados cinco níveis de inclusão (0; 0,625; 0,125; 0,25; 0,5g) dos resíduos, além do controle para o inóculo (CI), e seis amostras bacterianas, sendo duas delas Gram-positivas e quatro Gram-negativas. O método utilizado foi o de contagem de unidades formadoras de colônias em placas de petri (pour plate). Entre as bactérias Gram-positivas, o maior efeito inibidor foi detectado para amostras de Staphylococcus aureus, e maior susceptibilidade foi para a Escherichia coli dentre as Gram-negativas. Houve efeito antimicrobiano do resíduo da extração da própolis verde sobre as bactérias S. aureus e S. intermedius. O resíduo da extração da própolis marrom apresentou maior inibição para S. intermedius somente nos tratamentos com 0,5 e 0,25g de resíduo. Não houve efeito do resíduo da extração de própolis verde ou marrom sobre o crescimento de bactérias gênero Pseudomonas. Os resíduos da extração hidroalcoólica de própolis verde e marrom atuaram como inibidores de crescimento das bactérias Staphylococcus aureus, Staphylococcus intermedius e Escherichia coli, e em menor grau para Salmonella e Klebsiella. O resíduo tem maior efeito inibidor em bactérias Gram-positivas.

The addition of levels of ethanol extract of brown propolis was evaluated by assessing diet degradation in rumen fluid and predicting cumulative in vitro gas production by nonlinear (dual pool logistic and exponential) models. A total of 35 g of crude propolis were extracted in 65 mL of cereal alcohol (95% ethanol). In a completely randomized factorial design, the experimental diets combined four concentrations of extracted propolis diluted in cereal alcohol (0, 50, 70, and 100% of propolis extract) and supplementation doses (4, 8, 12, 16, and 20 mL/kg dry matter), tested in triplicate. Diet (400 g/kg Tifton hay and 600 g/kg concentrate) was incubated for 96 h carried out three times in three different weeks. There was significant interaction between extract concentration and dose on the dry matter (DM) degradability. Dry matter degradability of diet decreased exponentially as a function of the increase in dose (y = 678.55×dose-0.271). Pure alcohol treatment showed a negative exponential effect, with degradability of 303.61 g/kg when administered at a dose of 20 mL/kg DM. Treatment 100% ethanol extract reached the greatest degradability, estimated at 18.93 mL/kg DM. The treatment with 70% extract showed 6.35 mL/kg DM and the 50% extract, 7.65 mL/kg DM of minimum degradability. The reduction potential of pure ethanol was-0.32 mL gas/mL. Estimates of maximum gas production by dual pool logistic and exponential models were 13.10 mL and 12.07 mL for 100% extract, respectively. The 100% extract produced the highest gas production estimates, above 30 mL gas/100 mg DM of fermented diet. The degradation and fermentation of ruminant diet can be improved using 13 mL/DM kg of ethanol extract of propolis.

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-The present study tested brown propolis in crude or extract form as a feed supplement for feedlot lambs to identify the type that most improves in vivo nutrient digestibility. Digestibility was assessed by both total fecal collection and internal markers and the results obtained by these techniques were compared. The completely randomized design was used to compare feed intake and nutrient digestibility of 24 male lambs aged seven months among four dietary treatments (crude brown propolis, propolis ethanol extract, monensin sodium, and control). Methods of feces collection were compared using a completely randomized split-plot design, with experimental diets corresponding to the main factor and the methods to estimate fecal production as the sub-factor. The diets had a roughage:concentrate ratio of 50:50, with Tifton-85 bermudagrass hay (Cynodon spp.) as roughage, and ground corn, soybean meal and minerals as concentrate. The lambs fed diets with crude propolis had higher feed intake than those fed diets containing monensin sodium. The different diets did not affect dry matter, organic matter, crude protein, neutral detergent fiber, or acid detergent fiber digestibility, but crude propolis supplementation provided higher ether extract digestibility than monensin sodium. Nutrient digestibility, as indicated by indigestible neutral detergent fiber, indigestible acid detergent fiber and sequentially-determined indigestible acid detergent fiber, was lower than that found with the fecal collection method. The addition of brown propolis has the same effect as monensin, but neither maximized nutrient availability in the diet of feedlot lambs at 7 months of age. Digestibility assessment using the internal markers indigestible neutral detergent fiber, indigestible acid detergent fiber and sequentially-determined indigestible acid detergent fiber is not an efficient method compared with total feces collection.

Increasing demands for healthy and eco-friendly products and processes concerned with animal well-being have stimulated consumer interest in pasture finishing systems for lambs (Jacques, Berthiaume, & Cinq-Marsa, 2011). This feeding system affects animal growth rate, carcass weight and yield, muscle: fat ratio, and meat lipid profile, which can reflect in sensorial characteristics such as flavor, odor, and softness (Carrasco et al., 2009). Blackburn, Snowder, and Glimp (1991) reported lower amounts of fat in the meat of exclusively pasture-raised lambs compared to animals submitted to concentrate-based diets, being an attractive attribute for consumers. However, the meat industry is interested in heavy carcasses of young animals with good musculature, providing good cut yield and a minimum fat layer to protect carcasses while in cold storage. Tropical pasture exclusive feeding, such as Brachiaria spp.

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This study aimed to identify the best green-leaf offerings of Urochloa brizantha cv. Marandu for pasture-finished lambs receiving protein-energy supplementation at the rate of 1.6% of their body weights (BW). The animals were assigned into four treatments, which consisted of varied green-leaf offerings, namely, 105, 90, 75, or 60 g of dry matter/kg of body weight (BW). Sixty-eight contemporary Texel × mixed-breed lambs with an average initial BW of 22.6 kg were used over two years of experiment. The experiment was set up in a completely randomized design, the data were evaluated by analysis of variance and the means were compared by Tukey's test at the 0.05 significance level. There was no significant effect of year neither interaction of year with green-leaf offer. There were significant effects of leaf offer on dry matter intake (DMI) with behavior of positively linear (p<0.05). There were significant effects of green-leaf offer on final BW and average daily gain, and lambs receiving of 75 g/kg green-leaf offer showed the highest ADG (179 g/d). There were no significant differences in the carcass, meat, and primal cuts between the groups of lambs receiving different herbage offerings. There were significant effects of leaf offer on slaughter weight, hot carcass weight, and subcutaneous fat thickness. Lambs receiving of 75 g/kg leaf offer showed the highest SFT (5.8 mm). Slaughter weight and hot carcass weight averaged 41.52 kg and 20.07 kg, respectively. We recommend the treatment with leaves offer of 75 g/kg BW of Marandu pasture for the finishing of lambs supplemented at 1.6% of BW.