Effects of 2,4-D on the germination of megaspores and initial development of Regnellidium diphyllum Lindman (Monilophyta, Marsileaceae)

Cassanego, Mara Betânia Brizola; Droste, Annette; Windisch, Paulo G.

doi:10.1590/s1519-69842010005000014

Cited by 20 publications

(12 citation statements)

References 13 publications

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“…Assim, alguns fatores não considerados neste estudo podem estar influenciando a comunidade de samambaias no sítio, como o spray dos agrotóxicos lançados nas plantações de hortaliças da área lindeira e que, por ação do vento, podem estar sendo carregados ao interior do fragmento, acarretando decrés-cimo das espécies de samambaias epifíticas e de sua cobertura. Em estudo desenvolvido por Cassanego et al (2010), levando em conta a ação de metais pesados encontrados em herbicidas sobre a germinação de megásporos e desenvolvimento esporofítico inicial de Regnellidium diphyllum Lindm., constatou-se o efeito deletério tanto na germinação quanto no desenvolvimento inicial dessa samambaia. Becker et al (2015) verificaram menor diversidade de espécies epifíticas, incluindo samambaias em ambientes com mais concentração de metais pesados.…”

Section: Os Resultados Sustentam Que O Interior Do Macacounclassified

Impacto do efeito de borda sobre a comunidade de samambaias epifíticas em floresta com araucária

Silva

Mallmann

Cunha

et al. 2017

Rev. Bras. Ciênc. Ambient. (Online)

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Section: Os Resultados Sustentam Que O Interior Do Macacounclassified

Impacto do efeito de borda sobre a comunidade de samambaias epifíticas em floresta com araucária

Silva

Mallmann

Cunha

et al. 2017

Rev. Bras. Ciênc. Ambient. (Online)

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“…The species grows in wetlands, the leaf lamina frequently floating and the rhizome developing in the humid soil or mud. Previous studies reported effects of heavy metals and other pollutants on the early development of R. diphyllum (Wunder et al, 2009;Cassanego et al, 2010;Droste et al, 2010;Kieling-Rubio et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Effects of nickel on the fern Regnellidium diphyllum Lindm. (Marsileaceae)

Droste

2012

Braz. J. Biol.

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The heterosporous fern Regnellidium diphyllum occurs in southern Brazil and some adjoining localities in Uruguay and Argentina. Currently it is on the list of threatened species in the state of Rio Grande do Sul. Anthropic alterations such as the conversion of wetlands into agricultural areas or water and soil contamination by pollutants may compromise the establishment and survival of this species. Nickel (Ni) is an essential nutrient for plants but increasing levels of this metal due to pollution can cause deleterious effects especially in aquatic macrophytes. Megaspore germination tests were performed using Meyer's solution, at concentrations of 0 (control), 0.05, 0.5, 1, 5, 10, 20, 30, 50 and 100 mg L -1 of Ni. The initial development of apomictic sporophytes was studied using solutions containing 0 (control) to 4.8 mg L -1 of Ni. A significant negative relation was observed between the different Ni concentrations and the megaspore germination/ sporophyte formation rates. Primary roots, primary leaves and secondary leaves were significantly shorter at 3.2 and 4.8 mg L -1 of Ni, when compared with the treatment without this metal. At 4.8 mg L -1, leaves also presented chlorosis and necrosis. The introduction of pollutants with Ni in the natural habitat of Regnellidium diphyllum may inhibit the establishment of plants in the initial stage of development, a problem to be considered in relation to the conservation of this species.Keywords: heavy metals, megaspore, germination, sporophyte, pollution. Efeitos do níquel sobre a filicínea Regnellidium diphyllum Lindm. (Marsileaceae) ResumoA samambaia heterosporada Regnellidium diphyllum Lindm. ocorre no Sul do Brasil e em algumas localidades vizinhas no Uruguai e na Argentina. Atualmente, consta da lista de espécies ameaçadas do Estado do Rio Grande do Sul. Alterações antrópicas -tais como a conversão de áreas úmidas em áreas de agricultura ou a contaminação de água e solo por poluentes -podem comprometer o estabelecimento e a sobrevivência da espécie. Níquel (Ni) é um nutriente essencial às plantas, mas níveis aumentados desse metal, em razão da poluição, podem causar efeitos deletérios, especialmente em macrófitas aquáticas. Testes de germinação de megásporos foram realizados usando-se solução de Meyer, em concentrações de 0 (controle); 0,05; 0,5; 1; 5; 10; 20; 30; 50; 100 mg L -1 de Ni. O desenvolvimento inicial de esporófitos apomíticos foi estudado usando-se soluções contendo 0 (controle) a 4,8 mg L -1 de Ni. Relação negativa significante foi observada entre diferentes concentrações de Ni e as taxas de germinação de megásporos/formação de esporófitos. Raízes primárias e folhas primárias e secundárias foram significantemente mais curtas a 3,2 e 4,8 mg L -1 de Ni, quando comparadas ao tratamento sem esse metal. Em 4,8 mg L -1 , folhas também apresentaram clorose e necrose. A introdução de poluentes com Ni no habitat natural de Regnellidium diphyllum pode inibir o estabelecimento de plantas em estádio inicial de desenvolvimento, um problemaa a ser consider...

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“…In vitro culture methods provide controlled conditions for propagating sporophytes and studying fern biology, since germination and growth requirements of the Neotropical species are poorly understood (Fernández et al, 1999;Cassanego et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Spore germination and gametophyte development of Cyathea atrovirens (Langsd. & Fisch.) Domin (Cyatheaceae) under different pH conditions

2010

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Cyathea atrovirens (Langsd. & Fisch.) Domin, an intensely exploited tree fern, is found inside forests in several succession stages, as well as in swamps, roadsides and unused fields in the Rio dos Sinos basin, in the state of Rio Grande do Sul, southern Brazil. This study evaluated the in vitro germination and gametophyte development of C. atrovirens under different pH conditions, as well as spore viability after different storage times at 7 ºC. The lowest germination rate of spores was obtained at pH 7.0. At pH 5.0 to 6.5, laminar gametophyte development started at 20 to 30 days of culture. Antheridia and archegonia were first observed at 35 and 128 days, respectively. Storage at 7 o C did not affect germination rates. The capability of germination at different pH levels may explain the occurrence of the species in a wide range of habitats. The present study contributes to the understanding of the full life-cycle of C. atrovirens and to the analysis of the influence of abiotic components, providing information for the cultivation, management and conservation of the species.Keywords: tree ferns, gametophyte, pH, reproduction, conservation. Rechenmacher, C., Schmitt, JL. and Droste, A. through lens cleaning tissue. Part of the spores were stored in tubes at 7 ± 1 °C for later viability analysis. Spores did not undergo asepsis to keep experimental conditions close to those found in nature. Germinação de esporos e desenvolvimento gametofítico deTo evaluate the effect of pH on germination and initial gametophyte development of fresh spores, seven different pH levels were used: 4.0; 4.5; 5.0; 5.5; 6.0; 6.5; and 7.0. In a laminar flow hood, 10-mg samples of spores were distributed in flasks with 50-mL autoclaved Meyer solution (Meyer et al., 1955). Six replicates were used for each pH level, totalising 42 flasks. The cultures were maintained in a growth chamber at 26 ± 1 °C for a 16-hour photoperiod and photon flux density of 100 µmol.m -2.s -1 provided by cool white fluorescent tubes.Germination was scored at three, six, nine and 12 days of in vitro culture. Six slides (one slide per flask) from each treatment were analysed, verifying 100 spores on each slide. To follow up initial gametophyte development at different pH levels, 10 individuals on each slide (one slide per flask) were photographed and classified according to their developmental stage in the following categories at six, nine and 12 days of culture: a) rhizoid and chlorocyte; b) filamentous stage; and c) laminar stage. At 104, 128 and 150 days, the morphology of one individual from each flask was described.To describe the in vitro development of this species, only gametophytes obtained at pH 6.0 were evaluated at up to 128 days of culture. Representative individuals at different development stages were photographed, and the images were digitalised using a Canon EOS 220 camera coupled to an Olympus CX4 microscope.In a second experiment, the germinability rates of fresh and stored spores (90 and 180 days at 7 °C) were compared, using cultures w...

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Effects of 2,4-D on the germination of megaspores and initial development of Regnellidium diphyllum Lindman (Monilophyta, Marsileaceae)

Cited by 20 publications

References 13 publications

Impacto do efeito de borda sobre a comunidade de samambaias epifíticas em floresta com araucária

Impacto do efeito de borda sobre a comunidade de samambaias epifíticas em floresta com araucária

Effects of nickel on the fern Regnellidium diphyllum Lindm. (Marsileaceae)

Spore germination and gametophyte development of Cyathea atrovirens (Langsd. & Fisch.) Domin (Cyatheaceae) under different pH conditions

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