Genetic diversity and conservation of native populations of Maytenus Ilicifolia Mart. ex Reiss

Abstract

The aim of this work was to analyze genetic variability in 18 populations of Maytenus ilicifolia, and representatives of Maytenus aquifolia and Maytenus evonymoidis, collected in the states of Mato Grosso do Sul, Paraná, Santa Catarina and Rio Grande do Sul, using RAPD molecular markers. Considering total samples of the three species, 263 amplified fragments were identified, of which 72.2% showed to be polymorphous. The index of similarity (Jaccard coefficient) was on average 0.64 between M. ilicifolia and M. aquifolia; 0.47 between M. ilicifolia and M. evonymoidis; and 0.44 between M. aquifolia and M. evonymoidis. The analysis of groupings by the UPGMA algorithm allowed to clearly separate the three analyzed species. In determining the variability in M. ilicifolia, 222 bands were identified, on average 11.1 bands per primer, being 43.2% polymorphous. The index of similarity (Jaccard coefficient) in the bulks of each population in M. ilicifolia was, on average, 0.92 and the index of similarities among the populations was 0.83. The analysis of groupings with the UPGMA algorithm and the analysis of the main coordination (PCO), allowed the separation of the analyzed populations into three groups, the populations from the south of Rio Grande do Sul and the population from Mato Grosso do Sul standing out. A relation between the groupings found and the edaphoclimatic conditions of the collecting places was observed.Keywords: conservation genetics, Espinheira Santa, native populations, molecular markers. Diversidade

Genetic diversity in populations of Maytenus dasyclada (Celastraceae) in forest reserves and unprotected Araucaria forest remnants

“…For RAPD, genomic DNA was extracted as described in Mossi et al (2009) with minor modifications. The foliar tissues were macerated in liquid nitrogen, mixed with CTAB buffer (2 % CTAB, 1.4 M NaCl, 20 mM EDTA, 100 mM TrisHCl pH 8.0, 0.2 % 2-mercaptoethanol) and incubated at 65 °C for 30 min.…”

Correlation Among Metallic Ions, Phenolic Compounds and Antimicrobial Action in Medicinal Plants Extracts

“…These plants are widely cultivated in Guarapuava (Paraná state, Brazil) and used by local population and worldwide to treat several diseases. Ethnobotanical studies performed in some communities in the Paraná state mentioned these medicinal plants, among others, as frequently used by local populations (Jacoby et al 2002;Marchese et al 2004Marchese et al , 2009Gazim et al 2007;Mossi et al 2009). For example, M. ilicifolia is commonly consumed as a tea or mixed with Ilex paraguariensis (erva-mate) in the traditional beverage known as "Chimarrão."…”