Electroantennography in the study of two stingless bee species (Hymenoptera: meliponini)

Patricio, E.F.L.R.A.; Cruz‐López, Leopoldo; Morgan, E. David

doi:10.1590/s1519-69842004000500012

Cited by 6 publications

(4 citation statements)

References 14 publications

(12 reference statements)

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“…Moreover, several studies have demonstrated that terpenes are perceived by insects [62,66]. For example, studying antenna response has demonstrated that many pollinators can detect terpenes, including hawkmoths [66,67], honeybees [68,69], and stingless bees [70,71]. Another study showed that terpenes are emitted from the petals, floral nectaries, stigmas, and anthers of Arabidopsis flowers, which function as both a defensive mechanism against pathogens and also as short-distance attractants for insect pollinators [72].…”

Section: Histochemistrymentioning

confidence: 99%

Pollination and Floral Biology of a Rare Morning Glory Species Endemic to Thailand, Argyreia siamensis

Jirabanjongjit

Traiperm

Sando³

et al. 2021

Plants

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Argyreia siamensis is extremely rare, and very little is known about its reproduction. The species has colorful flowers that seem likely to attract pollinators, but population sizes are typically small (<30 individuals). To determine whether poor reproduction contributes to its rarity, we investigated its mating system and potential pollinators in two populations. We also examined the staminal trichomes and floral nectary to investigate their role in pollinator attraction. The mating system was assessed with a bagging experiment and pollinator visits were recorded with action cameras. Additionally, we tested the staminal trichomes and floral nectary for terpenes and flavonoids and examined floral nectary micromorphology via scanning electron microscope and compound light microscope. Our results reveal that A. siamensis is self-incompatible and dependent on pollinators; the western population was pollinated by bees (Meliponini and Amegilla), while the eastern population was mainly pollinated by skipper butterflies (Hesperiidae). Both staminal trichomes and the floral nectary appear to contribute to pollinator attraction through the presence of terpenes and flavonoids (in both secretory structures) and nectariferous tissue and nectarostomata (in the nectary). Our results indicate that A. siamensis has reliable and effective pollinators and that insufficient pollination is likely not a primary cause of its rarity.

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Section: Histochemistrymentioning

confidence: 99%

Pollination and Floral Biology of a Rare Morning Glory Species Endemic to Thailand, Argyreia siamensis

Jirabanjongjit

Traiperm

Sando³

et al. 2021

Plants

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“…Further, response could be ascribed to the existence of 2-heptanol plus 2-nonanol in the mandibular glands of both bees, and to humulene, β-caryophyllene and α-cubebene, emitted by the tibia and being present in their cerumen. [114] A blend of terpenoids and oxygenated components as well as hydrocarbons was found in the secretions of the bee species Melipona beecheeii. In contrast to the 11 Brazilian species being investigated at that time, 2-heptanol could not be proved in the mandibular gland releases, but on the other hand, increased amounts of oxides were detected.…”

Section: Pheromones Of Beesmentioning

confidence: 99%

“…The two Brazilian Frieseomelitta species F. silvestrii and F. varia both demonstrated that the bees reacted to head and abdominal extracts, to their own extracts as well as to the extracts of the other species, and to the posterior tibia extracts of F. silvestrii . Further, response could be ascribed to the existence of 2‐heptanol plus 2‐nonanol in the mandibular glands of both bees, and to humulene, β ‐caryophyllene and α ‐cubebene, emitted by the tibia and being present in their cerumen …”

Section: Pheromones Of Insectsmentioning

confidence: 99%

Essential oil components as pheromones. A review.

Müller

Buchbauer

2011

Flavour & Fragrance J

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Studies regarding pheromes consisting of essential oil components of various plants and released by insects and arachnids (pests) were compiled for this review. For example, verbenol and verbenone were found to be an essential part of the bark beetle's aggregation pheromone, released with the intent to round up its conspecifics. Concerning plant lice, aphids use (E)-β-farnesene as an alarm pheromone. Furthermore, nepetalactol and nepetalactone, two essential oil components of the cat mint (Lamiaceae), occur as an aphid-released sex pheromone, emitted to induce mating behaviour. On the other hand, some bees imitate a linalool-containing orchid odour as their sex pheromone and thus attract their mating partners. The sand fly, responsible for the American visceral leishmaniasis, uses derivatives of either α-himachalene, germacrene D or cembrene as a sex pheromone. Studies about termites reveal a large amount of different presumable pheromone compounds that also appear as essential oil components, e.g. neocembrene as a part of the trail pheromone, (E,E)-α-farnesene as an alarm pheromone and other terpenes, such as for example γ-cadinene as primer pheromone, enforcing the effect of the juvenile hormone and therefore determining about caste development. Concerning the pest insects, management strategies, conducted with pheromone traps, are presented or compared. Regarding arachnids, studies about spider pheromones are rare, whereas more information is available about mites, which release neral and geranial as an alarm pheromone.

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“…This compound was previously reported as the major compound in the alarm pheromone of some stingless bees (Schorkopf 2016) Chiral compounds as secondary alcohols play an important role in alarm pheromone communication (Schorkopf et al 2009;Schorkopf et al 2011;Leonhardt 2017). Studies evaluating them have been conducted with some bee species (Patricio et al 2004). For instance, honey bee workers (Apis mellifera ) were able to discriminate between queen-and worker-laid eggs detecting branched hydrocarbon stereoisomers (Katzav- .01 EQ (Kruskal-Wallis H = 1.00, df = 1, P = 0.32), 0.1 EQ (Kruskal-Wallis H = 1.70, df = 1, P = 0.19), 1 EQ (Kruskal-Wallis H = 6.74, df = 1, P < 0.01), 10 EQ (Kruskal-Wallis H = 1.46, df = 1, P = 0.23).…”

Section: Discussionmentioning

confidence: 99%

(S)-2-Heptanol, the alarm pheromone of the stingless bee Melipona solani (Hymenoptera, Meliponini)

et al. 2019

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Timely defence response is critical in any highly social bee species. Chemical signalling is closely linked to this behaviour, and several pheromones have been discovered. In this work, we identified the pheromone of the stingless bee Melipona solani and evaluated the electrophysiological and behavioural response of the bee to the identified compound. We determined that the mandibular glands serve as the reservoir of the alarm pheromone. Because enantiomeric recognition has been scarcely examined, we performed bioassays and electroantennographic (EAG) studies to determine the role of chirality in pheromone recognition. We found that (S)-2-heptanol was the active compound involved in the alarm response of this species. Although we did not find (R)-2-heptanol in the mandibular gland extracts, bees responded to it in a similar way to the (S)-isomer and to a racemic mixture. The behavioural response of M. solani was flight, different from other Melipona species behaviour. We discuss these findings in an evolutionary and ecological framework.

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Electroantennography in the study of two stingless bee species (Hymenoptera: meliponini)

Cited by 6 publications

References 14 publications

Pollination and Floral Biology of a Rare Morning Glory Species Endemic to Thailand, Argyreia siamensis

Pollination and Floral Biology of a Rare Morning Glory Species Endemic to Thailand, Argyreia siamensis

Essential oil components as pheromones. A review.

(S)-2-Heptanol, the alarm pheromone of the stingless bee Melipona solani (Hymenoptera, Meliponini)

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