Colonization of rice and Spodoptera frugiperda J.E. Smith (Lepidoptera: Noctuidae) larvae by genetically modified endophytic Methylobacterium mesophilicum

Rampelotti-Ferreira, Fátima Teresinha; Ferreira, Anderson; Vendramim, José Djair; Lacava, Paulo Teixeira; Azevedo, João Lúcio; Araújo, Welington Luiz

doi:10.1590/s1519-566x2010000200026

Cited by 7 publications

(3 citation statements)

References 7 publications

(8 reference statements)

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“…The most closely related sequences are from bacteria that belong to the α- and β-Proteobacteria, two of which are Methylobacterium species, free-living epiphytic bacteria known to establish endophytic colonies. These species are reported to be transferred from the plant to phytophagous insects and to survive inside arthropod hosts (Kutschera, 2007; Rampelotti-Ferreira et al, 2010), facilitating a potential horizontal gene transfer. Alternatively, Proteobacteria are known endosymbionts of arthropods and often reside in the reproductive organs for vertical transmission to following generations (Wernegreen, 2002).…”

Section: Resultsmentioning

confidence: 99%

A gene horizontally transferred from bacteria protects arthropods from host plant cyanide poisoning

Wybouw

Dermauw

Tirry

et al. 2014

eLife

137

144

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Cyanogenic glucosides are among the most widespread defense chemicals of plants. Upon plant tissue disruption, these glucosides are hydrolyzed to a reactive hydroxynitrile that releases toxic hydrogen cyanide (HCN). Yet many mite and lepidopteran species can thrive on plants defended by cyanogenic glucosides. The nature of the enzyme known to detoxify HCN to β-cyanoalanine in arthropods has remained enigmatic. Here we identify this enzyme by transcriptome analysis and functional expression. Phylogenetic analysis showed that the gene is a member of the cysteine synthase family horizontally transferred from bacteria to phytophagous mites and Lepidoptera. The recombinant mite enzyme had both β-cyanoalanine synthase and cysteine synthase activity but enzyme kinetics showed that cyanide detoxification activity was strongly favored. Our results therefore suggest that an ancient horizontal transfer of a gene originally involved in sulfur amino acid biosynthesis in bacteria was co-opted by herbivorous arthropods to detoxify plant produced cyanide.DOI: http://dx.doi.org/10.7554/eLife.02365.001

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Section: Resultsmentioning

confidence: 99%

A gene horizontally transferred from bacteria protects arthropods from host plant cyanide poisoning

Wybouw

Dermauw

Tirry

et al. 2014

eLife

137

144

View full text Add to dashboard Cite

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“…Assim, Rampelotti-Ferreira et al (2010) elaboraram um estudo voltado a avaliar a colonização endofítica não só de plântulas de arroz, como também de larvas de Spodoptera frugiperda, pela bactéria transformada [marcada com a proteína verde fluorescente (gfp)] Methylobacterium mesophilicum cepa SR1.6/6, in vitro. Os resultados das análises de microscopia fluorescente revelaram que a bactéria inoculada por meio das raízes mostrou-se capaz de colonizar as plântulas e de ser, então, transferida das plântu-las para as larvas durante a alimentação do inseto.…”

Section: Aplicação De Recombinantes Endofíticos Na Agrobiotecnologiaunclassified

Bactérias endofíticas como agentes de controle biológico na orizicultura

2016

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RESUMO Esta publicação apresenta alguns dados sobre as interações entre as bactérias e as plantas hospedeiras, considerando os simbiontes e os patógenos das plantas de importância agrícola, com ênfase nos agroecossistemas de arroz irrigado. Neste trabalho foram abordados aspectos relacionados a essas bactérias endofíticas, tais como: (i) endofíticos e suas interações com as plantas cultivadas; (ii) rizobactérias promotoras do crescimento vegetal [plant growth-promoting rhizobacteria (PGPR)] e sua aplicabilidade no controle biológico; (iii) bactérias endofíticas versus PGPR no controle biológico de fitopatógenos; (iv) aplicação de recombinantes endofíticos na agrobiotecnologia. No final do artigo estão apresentadas as opiniões e perspectivas dos autores sobre as bactérias que vivem associadas às plantas cultivadas nos agroecossistemas.

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“…A presença de microrganismos endofíticos no interior da planta hospedeira pode aumentar a aptidão e desempenho da planta, protegendo-a contra pragas e patógenos, melhorando seu crescimento e aumentando a resistência em ambientes com estresses (Azevedo et al, 2000, Scherwinski et al, 2007. Além da ocorrência natural de endofiticos, é possível desenvolver estratégias de controle a partir de bactérias endofíticas por modificações genéticas (Rampelotti-Ferreira et al, 2010;Quecine et al, 2014). Essas bactérias estão presentes em todas as espécies vegetais e podem colonizar ativamente de forma local ou sistêmica, ou então permanecendo em estado de latência, sendo capazes de ajudar a planta no crescimento e controle de pragas e doenças (Azevedo et al, 2002;Bloemberg e Lugtemberg, 2001;Dobbelaere et al, 2003;Lodewyckx et al, 2002).…”

Section: Introductionunclassified

Silenciamento gênico por interferência de RNA (RNAi) em traça-do-tomateiro, <i>Tuta absoluta</i> (Meyrick), utilizando bactérias expressando dupla fita de RNA (dsRNA)

Bento¹

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Gene silencing by RNA interference (RNAi) in tomato leafminer, Tuta absoluta (Meyrick), using bactéria expressing double-stranded RNA (dsRNA) The use of RNAi technique has been evaluated in several insect pests because it is an innovative strategy that can be integrated in the management of important agricultural pests. Insects of the Order Lepidoptera are recognized to present recalcitrance to gene silencing using dsRNA. Thus, adjustments should be done to dsRNA delivery methods to have molecule stability until it reaches the mRNA target for silencing in the insect. Gene silencing by RNAi has potential use to control the tomato leafminer Tuta absoluta (Meyrick, 1917) (Lepidoptera: Gelechiidae), one of the main insect pests of tomato crop worldwide. The objective of this work was to select and evaluate the silencing of T. absoluta genes using dsRNA delivery method via E. coli HT115 (DE3) bacterium, offered in artificial diet. Also, aiming the applicability of the use of bacteria growing in the same habitat of insect pests, we evaluate the colonization of the endophytic bacteria Pantoea agglomerans strain 33.1, Burkholderia sp. strain SCMS54 and Burkholderia ambifaria strain RZ2MS16 in tomato plants and T. absoluta larvae for further transformation and potential use as dsRNA delivery strategy for silencing target genes of T. absoluta. We evaluated eight genes of T. absoluta: juvenile hormone inducible protein-JHP; juvenile hormone epoxide hydrolase protein-JHEH; ecdysteroid 25-hydroxylase-PHM; chitin synthase A-CHI; glutathione S-transferase epsilon 2-GST; carboxylesterase-COE; alkaline phosphatase-AP and; arginine kinase-AK. Evaluating biological parameters (larval mortality, larval stage duration and pupal weight) and gene expression in five feeding periods, we proved the efficiency of the methodology in the evaluation of gene silencing by RNAi, and evaluated the effects of gene silencing on the development of T. absoluta. The genes AK, CHI and JHP presented positive results regarding gene silencing and larval mortality, being promising to use RNAi silencing as a strategy to control T. absoluta. Pantoea agglomerans showed good results colonizing "Micro-Tom" tomato plants and T. absoluta larvae, besides being present in tissues preferentially used by larvae for feeding. However, larvae did not show differences in larval mortality when feeding on tomato plants inoculated with transformed P. agglomerans.

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Colonization of rice and Spodoptera frugiperda J.E. Smith (Lepidoptera: Noctuidae) larvae by genetically modified endophytic Methylobacterium mesophilicum

Cited by 7 publications

References 7 publications

A gene horizontally transferred from bacteria protects arthropods from host plant cyanide poisoning

A gene horizontally transferred from bacteria protects arthropods from host plant cyanide poisoning

Bactérias endofíticas como agentes de controle biológico na orizicultura

Silenciamento gênico por interferência de RNA (RNAi) em traça-do-tomateiro, <i>Tuta absoluta</i> (Meyrick), utilizando bactérias expressando dupla fita de RNA (dsRNA)

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